Evaluation of the effect on antimicrobial properties, color, roughness and curing time of glass ionomer cement incorporated with silver and copper nanoparticles
Abstract The objective of this study was to investigate the antimicrobial potential of glass ionomer cement (GIC) incorporated with silver and copper nanoparticles (CuAgNP) in three different concentrations (GIC 0.01% CuAgNP, GIC 0.05% CuAgNP, and GIC 0.1% CuAgNP) on the inhibition of in vitro forma...
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Springer
2025-02-01
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| Series: | Discover Materials |
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| Online Access: | https://doi.org/10.1007/s43939-025-00188-9 |
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| author | Laís Aragão Lima Wanessa Fernandes Matias Regis Fábio Ruan Louzeiro Lima Victor Teixeira Noronha Amauri Jardim de Paula Fernanda Freitas Sousa Paulo Goberlânio Barros Silva Rafael Melo Freire Lidiany Karla Azevedo Rodrigues |
| author_facet | Laís Aragão Lima Wanessa Fernandes Matias Regis Fábio Ruan Louzeiro Lima Victor Teixeira Noronha Amauri Jardim de Paula Fernanda Freitas Sousa Paulo Goberlânio Barros Silva Rafael Melo Freire Lidiany Karla Azevedo Rodrigues |
| author_sort | Laís Aragão Lima |
| collection | DOAJ |
| description | Abstract The objective of this study was to investigate the antimicrobial potential of glass ionomer cement (GIC) incorporated with silver and copper nanoparticles (CuAgNP) in three different concentrations (GIC 0.01% CuAgNP, GIC 0.05% CuAgNP, and GIC 0.1% CuAgNP) on the inhibition of in vitro formation of duospecies biofilm of Streptococcus mutans and Candida albicans, surface roughness, color variation, and curing time. A control group was also included, consisting of GIC without nanoparticles. Duoespecie biofilms were grown on specimens of GIC in Tryptic Soy Broth placed in 24-well plates for 72 h. The biofilms were then collected and plated on Mitis-Salivarius-Bacitracin (MSB) agar and Sabouraud dextrose agar for Streptococcus mutans and Candida albicans, respectively. Colony-forming unit (CFU) counts and dry weight were measured to assess the biofilm growth. Physical properties were evaluated through tests on surface roughness, color variation, and curing time. The normality of the distribution was evaluated using the Kolmogorov–Smirnov test. Data were analyzed using ANOVA followed by Tukey’s test. The results showed that the addition of CuAgNP did not increase antimicrobial activity compared to the control (p > 0.05). Furthermore, longer curing time resulted in negative effects on physical properties, such as color variation and greater surface roughness (p < 0.05). The addition of CuAgNP did not improve the properties of GIC in this study. |
| format | Article |
| id | doaj-art-761175293b234d5d907a64d28919c87d |
| institution | Kabale University |
| issn | 2730-7727 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Materials |
| spelling | doaj-art-761175293b234d5d907a64d28919c87d2025-02-09T12:59:30ZengSpringerDiscover Materials2730-77272025-02-015111410.1007/s43939-025-00188-9Evaluation of the effect on antimicrobial properties, color, roughness and curing time of glass ionomer cement incorporated with silver and copper nanoparticlesLaís Aragão Lima0Wanessa Fernandes Matias Regis1Fábio Ruan Louzeiro Lima2Victor Teixeira Noronha3Amauri Jardim de Paula4Fernanda Freitas Sousa5Paulo Goberlânio Barros Silva6Rafael Melo Freire7Lidiany Karla Azevedo Rodrigues8Postgraduate Program in Odontology, Federal University of CearáPostgraduate Program in Odontology, Federal University of CearáPostgraduate Program in Odontology, Federal University of CearáPostgraduate Program in Physics, Federal University of CearáIIlum School of Science (CNPEM)Christus University Center, UNICHRISTUSChristus University Center, UNICHRISTUSFacultad de Ingeniería y Arquitectura, Universidad Central de ChilePostgraduate Program in Odontology, Federal University of CearáAbstract The objective of this study was to investigate the antimicrobial potential of glass ionomer cement (GIC) incorporated with silver and copper nanoparticles (CuAgNP) in three different concentrations (GIC 0.01% CuAgNP, GIC 0.05% CuAgNP, and GIC 0.1% CuAgNP) on the inhibition of in vitro formation of duospecies biofilm of Streptococcus mutans and Candida albicans, surface roughness, color variation, and curing time. A control group was also included, consisting of GIC without nanoparticles. Duoespecie biofilms were grown on specimens of GIC in Tryptic Soy Broth placed in 24-well plates for 72 h. The biofilms were then collected and plated on Mitis-Salivarius-Bacitracin (MSB) agar and Sabouraud dextrose agar for Streptococcus mutans and Candida albicans, respectively. Colony-forming unit (CFU) counts and dry weight were measured to assess the biofilm growth. Physical properties were evaluated through tests on surface roughness, color variation, and curing time. The normality of the distribution was evaluated using the Kolmogorov–Smirnov test. Data were analyzed using ANOVA followed by Tukey’s test. The results showed that the addition of CuAgNP did not increase antimicrobial activity compared to the control (p > 0.05). Furthermore, longer curing time resulted in negative effects on physical properties, such as color variation and greater surface roughness (p < 0.05). The addition of CuAgNP did not improve the properties of GIC in this study.https://doi.org/10.1007/s43939-025-00188-9Dental cariesAntimicrobial agentMetal nanoparticlesBiofilmsDental materials |
| spellingShingle | Laís Aragão Lima Wanessa Fernandes Matias Regis Fábio Ruan Louzeiro Lima Victor Teixeira Noronha Amauri Jardim de Paula Fernanda Freitas Sousa Paulo Goberlânio Barros Silva Rafael Melo Freire Lidiany Karla Azevedo Rodrigues Evaluation of the effect on antimicrobial properties, color, roughness and curing time of glass ionomer cement incorporated with silver and copper nanoparticles Discover Materials Dental caries Antimicrobial agent Metal nanoparticles Biofilms Dental materials |
| title | Evaluation of the effect on antimicrobial properties, color, roughness and curing time of glass ionomer cement incorporated with silver and copper nanoparticles |
| title_full | Evaluation of the effect on antimicrobial properties, color, roughness and curing time of glass ionomer cement incorporated with silver and copper nanoparticles |
| title_fullStr | Evaluation of the effect on antimicrobial properties, color, roughness and curing time of glass ionomer cement incorporated with silver and copper nanoparticles |
| title_full_unstemmed | Evaluation of the effect on antimicrobial properties, color, roughness and curing time of glass ionomer cement incorporated with silver and copper nanoparticles |
| title_short | Evaluation of the effect on antimicrobial properties, color, roughness and curing time of glass ionomer cement incorporated with silver and copper nanoparticles |
| title_sort | evaluation of the effect on antimicrobial properties color roughness and curing time of glass ionomer cement incorporated with silver and copper nanoparticles |
| topic | Dental caries Antimicrobial agent Metal nanoparticles Biofilms Dental materials |
| url | https://doi.org/10.1007/s43939-025-00188-9 |
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