Phycobacteria Biodiversity, Selected Isolation, and Bioactivity Elucidation of New Bacterial Species of Highly Toxic Marine Dinoflagellate <i>Alexandrium minutum</i> amtk4
Phycosphere niches host rich, unique microbial consortia that harbor complex algae–bacteria interactions with fundamental significance in underpinning most functions of aquatic ecological processes. Therefore, harvesting the uncultured phycobacteria is crucial for understanding the intricate mechani...
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2025-05-01
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| author | Xiaoling Zhang Zekang Pan Jinkai Zhang Bingqian Liu Qiao Yang |
| author_facet | Xiaoling Zhang Zekang Pan Jinkai Zhang Bingqian Liu Qiao Yang |
| author_sort | Xiaoling Zhang |
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| description | Phycosphere niches host rich, unique microbial consortia that harbor complex algae–bacteria interactions with fundamental significance in underpinning most functions of aquatic ecological processes. Therefore, harvesting the uncultured phycobacteria is crucial for understanding the intricate mechanisms governing these dynamic interactions. Here, we characterized and compared microbial community composition of the phycosphere microbiota from six harmful algal bloom-forming marine dinoflagellates, <i>Alexandrium</i> spp., and their bacterial associations. Furthermore, based on a combinational enhanced cultivation strategy (CECS) procedure for the selected isolation for cultivable phycobacteria, a new yellow-pigmented bioactive bacterium designated ABI-6-9 was successfully recovered from cultivable phycobacteria of the highly toxic <i>A. minutum</i> strain amtk4. An additional phylogenomic analysis fully identified this new isolate as a potential novel species of the genus <i>Mameliella</i> within the family <i>Roseobacteraceae</i>. The bioactivity evaluation observed that strain ABI-6-9 can significantly promote the cell growth of its algal host and altered the gonyautoxin accumulation profiles in the co-culture circumstance. Additionally, the bacterial production of active bioflocculanting exopolysaccharides (EPSs) by strain ABI-6-9 was also measured after culture optimization. Thus, these findings revealed the potential environmental and biotechnological implications of this new microalgae growth- promoting phycobacterium. |
| format | Article |
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| issn | 2076-2607 |
| language | English |
| publishDate | 2025-05-01 |
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| spelling | doaj-art-7609b219512e47e4a22044abd0943ac02025-08-20T02:21:04ZengMDPI AGMicroorganisms2076-26072025-05-01136119810.3390/microorganisms13061198Phycobacteria Biodiversity, Selected Isolation, and Bioactivity Elucidation of New Bacterial Species of Highly Toxic Marine Dinoflagellate <i>Alexandrium minutum</i> amtk4Xiaoling Zhang0Zekang Pan1Jinkai Zhang2Bingqian Liu3Qiao Yang4ABI Group, Phycosphere Microbiology Laboratory, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, ChinaABI Group, Phycosphere Microbiology Laboratory, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, ChinaABI Group, Phycosphere Microbiology Laboratory, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, ChinaABI Group, Phycosphere Microbiology Laboratory, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, ChinaABI Group, Phycosphere Microbiology Laboratory, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, ChinaPhycosphere niches host rich, unique microbial consortia that harbor complex algae–bacteria interactions with fundamental significance in underpinning most functions of aquatic ecological processes. Therefore, harvesting the uncultured phycobacteria is crucial for understanding the intricate mechanisms governing these dynamic interactions. Here, we characterized and compared microbial community composition of the phycosphere microbiota from six harmful algal bloom-forming marine dinoflagellates, <i>Alexandrium</i> spp., and their bacterial associations. Furthermore, based on a combinational enhanced cultivation strategy (CECS) procedure for the selected isolation for cultivable phycobacteria, a new yellow-pigmented bioactive bacterium designated ABI-6-9 was successfully recovered from cultivable phycobacteria of the highly toxic <i>A. minutum</i> strain amtk4. An additional phylogenomic analysis fully identified this new isolate as a potential novel species of the genus <i>Mameliella</i> within the family <i>Roseobacteraceae</i>. The bioactivity evaluation observed that strain ABI-6-9 can significantly promote the cell growth of its algal host and altered the gonyautoxin accumulation profiles in the co-culture circumstance. Additionally, the bacterial production of active bioflocculanting exopolysaccharides (EPSs) by strain ABI-6-9 was also measured after culture optimization. Thus, these findings revealed the potential environmental and biotechnological implications of this new microalgae growth- promoting phycobacterium.https://www.mdpi.com/2076-2607/13/6/1198phycosphere microbiologyalgae–bacteria interactionsexopolysaccharidescultivable phycobacteriamicroalgae growth-promoting bacteriumtoxic dinoflagellate |
| spellingShingle | Xiaoling Zhang Zekang Pan Jinkai Zhang Bingqian Liu Qiao Yang Phycobacteria Biodiversity, Selected Isolation, and Bioactivity Elucidation of New Bacterial Species of Highly Toxic Marine Dinoflagellate <i>Alexandrium minutum</i> amtk4 Microorganisms phycosphere microbiology algae–bacteria interactions exopolysaccharides cultivable phycobacteria microalgae growth-promoting bacterium toxic dinoflagellate |
| title | Phycobacteria Biodiversity, Selected Isolation, and Bioactivity Elucidation of New Bacterial Species of Highly Toxic Marine Dinoflagellate <i>Alexandrium minutum</i> amtk4 |
| title_full | Phycobacteria Biodiversity, Selected Isolation, and Bioactivity Elucidation of New Bacterial Species of Highly Toxic Marine Dinoflagellate <i>Alexandrium minutum</i> amtk4 |
| title_fullStr | Phycobacteria Biodiversity, Selected Isolation, and Bioactivity Elucidation of New Bacterial Species of Highly Toxic Marine Dinoflagellate <i>Alexandrium minutum</i> amtk4 |
| title_full_unstemmed | Phycobacteria Biodiversity, Selected Isolation, and Bioactivity Elucidation of New Bacterial Species of Highly Toxic Marine Dinoflagellate <i>Alexandrium minutum</i> amtk4 |
| title_short | Phycobacteria Biodiversity, Selected Isolation, and Bioactivity Elucidation of New Bacterial Species of Highly Toxic Marine Dinoflagellate <i>Alexandrium minutum</i> amtk4 |
| title_sort | phycobacteria biodiversity selected isolation and bioactivity elucidation of new bacterial species of highly toxic marine dinoflagellate i alexandrium minutum i amtk4 |
| topic | phycosphere microbiology algae–bacteria interactions exopolysaccharides cultivable phycobacteria microalgae growth-promoting bacterium toxic dinoflagellate |
| url | https://www.mdpi.com/2076-2607/13/6/1198 |
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