Immunoassay of infectious agents
Immunoassays have evolved for a broad range of applications since the pioneering work of Yalow and Berson who developed the first competitive radioimmunoassay (RIA) for human insulin in 1959. Immunoassay detection of specific antigens and host-produced antibodies directed against such antigens const...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2003-10-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03354ss02 |
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| _version_ | 1850152546330476544 |
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| author | Peter E. Andreotti George V. Ludwig Anne Harwood Peruski James J. Tuite Stephen S. Morse Leonard F. Peruski |
| author_facet | Peter E. Andreotti George V. Ludwig Anne Harwood Peruski James J. Tuite Stephen S. Morse Leonard F. Peruski |
| author_sort | Peter E. Andreotti |
| collection | DOAJ |
| description | Immunoassays have evolved for a broad range of applications since the pioneering work of Yalow and Berson who developed the first competitive radioimmunoassay (RIA) for human insulin in 1959. Immunoassay detection of specific antigens and host-produced antibodies directed against such antigens constitutes one of the most widely used and successful methods for diagnosing infectious diseases (IDs). The number and variety of new assay systems that are continually being developed reflect the increasing demand for immunoassays possessing greater sensitivity, speed, and ease of use. This trend has been driven, in part, by the need for improved immunodiagnostic systems to perform rapid testing and counter emerging IDs and biothreat (BT) agents. Another factor driving this trend is the need to integrate immunoassays with more sensitive nucleic acid-based methods for a comprehensive approach. Here we examine the development of immunoassays, some of the key formats used for the detection and identification of BT/ID agents, and the application of these technologies under different scenarios. |
| format | Article |
| id | doaj-art-75f06f3cf2d2403d9bb04c9ed1a0e3b1 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2003-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-75f06f3cf2d2403d9bb04c9ed1a0e3b12025-08-20T02:25:57ZengTaylor & Francis GroupBioTechniques0736-62051940-98182003-10-0135485085910.2144/03354ss02Immunoassay of infectious agentsPeter E. Andreotti0George V. Ludwig1Anne Harwood Peruski2James J. Tuite3Stephen S. Morse4Leonard F. Peruski51ASD Biosystems, Richmond, VA2United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD3Indiana University School of Medicine, Northwest Center, Gary, IN1ASD Biosystems, Richmond, VA4Mailman School of Public Health, Columbia University, New York, NY, USA3Indiana University School of Medicine, Northwest Center, Gary, INImmunoassays have evolved for a broad range of applications since the pioneering work of Yalow and Berson who developed the first competitive radioimmunoassay (RIA) for human insulin in 1959. Immunoassay detection of specific antigens and host-produced antibodies directed against such antigens constitutes one of the most widely used and successful methods for diagnosing infectious diseases (IDs). The number and variety of new assay systems that are continually being developed reflect the increasing demand for immunoassays possessing greater sensitivity, speed, and ease of use. This trend has been driven, in part, by the need for improved immunodiagnostic systems to perform rapid testing and counter emerging IDs and biothreat (BT) agents. Another factor driving this trend is the need to integrate immunoassays with more sensitive nucleic acid-based methods for a comprehensive approach. Here we examine the development of immunoassays, some of the key formats used for the detection and identification of BT/ID agents, and the application of these technologies under different scenarios.https://www.future-science.com/doi/10.2144/03354ss02 |
| spellingShingle | Peter E. Andreotti George V. Ludwig Anne Harwood Peruski James J. Tuite Stephen S. Morse Leonard F. Peruski Immunoassay of infectious agents BioTechniques |
| title | Immunoassay of infectious agents |
| title_full | Immunoassay of infectious agents |
| title_fullStr | Immunoassay of infectious agents |
| title_full_unstemmed | Immunoassay of infectious agents |
| title_short | Immunoassay of infectious agents |
| title_sort | immunoassay of infectious agents |
| url | https://www.future-science.com/doi/10.2144/03354ss02 |
| work_keys_str_mv | AT petereandreotti immunoassayofinfectiousagents AT georgevludwig immunoassayofinfectiousagents AT anneharwoodperuski immunoassayofinfectiousagents AT jamesjtuite immunoassayofinfectiousagents AT stephensmorse immunoassayofinfectiousagents AT leonardfperuski immunoassayofinfectiousagents |