Lactobacillus rhamnosus-derived extracellular vesicles influence calcium deposition in a model of breast cancer intraductal calcium stress
Summary: Extracellular calcium export by the breast ductal epithelium is crucial during lactation and plays a significant role in breast cancer progression. Intraductal calcium deposition is a hallmark of aggressive premalignant lesions. This study tested the hypothesis that microbiome-derived extra...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225007990 |
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| author | Ngoc Vuong Melany Alomia Ahana Byne Purva Gade Thomas Raymond Philipson Rayan Ibrahim Alhammad Cade J. Skislak Intisar Alruwaili Fahad M. Alsaab Weidong Zhou Marissa Howard Andrea Brothers Amanda Haymond Still Robyn P. Araujo Monique Van Hoek Barbara Birkaya Virginia Espina Richard A. Hoefer Lance Liotta Alessandra Luchini |
| author_facet | Ngoc Vuong Melany Alomia Ahana Byne Purva Gade Thomas Raymond Philipson Rayan Ibrahim Alhammad Cade J. Skislak Intisar Alruwaili Fahad M. Alsaab Weidong Zhou Marissa Howard Andrea Brothers Amanda Haymond Still Robyn P. Araujo Monique Van Hoek Barbara Birkaya Virginia Espina Richard A. Hoefer Lance Liotta Alessandra Luchini |
| author_sort | Ngoc Vuong |
| collection | DOAJ |
| description | Summary: Extracellular calcium export by the breast ductal epithelium is crucial during lactation and plays a significant role in breast cancer progression. Intraductal calcium deposition is a hallmark of aggressive premalignant lesions. This study tested the hypothesis that microbiome-derived extracellular vesicles (EVs) influence calcium modulation in premalignant breast cancer lesions. Based on the analysis of plasma, serum, saliva, and tissue collected from breast cancer patients and controls (N = 150), Lactobacillus rhamnosus (Lr) was chosen as the model microbiota. In a BT-474 human breast cancer cell line monolayer culture under acute calcium stress, Lr EVs enhanced intracellular calcium intake. In a BT474 3D spheroid model under chronic calcium stress, Lr EVs increased extracellular calcium deposition and mRNA expression of calcium export channel plasma membrane calcium-transporting ATPase 2 (PMCA2) and stromal interaction molecule 1 (STIM1) in a dose-dependent manner. We propose that Lr EVs influence calcium regulation and mineral deposition, thereby affecting premalignant breast cancer progression. |
| format | Article |
| id | doaj-art-75de79ac8f55483fba11f0fa10a068fe |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-75de79ac8f55483fba11f0fa10a068fe2025-08-20T03:47:32ZengElsevieriScience2589-00422025-06-0128611253810.1016/j.isci.2025.112538Lactobacillus rhamnosus-derived extracellular vesicles influence calcium deposition in a model of breast cancer intraductal calcium stressNgoc Vuong0Melany Alomia1Ahana Byne2Purva Gade3Thomas Raymond Philipson4Rayan Ibrahim Alhammad5Cade J. Skislak6Intisar Alruwaili7Fahad M. Alsaab8Weidong Zhou9Marissa Howard10Andrea Brothers11Amanda Haymond Still12Robyn P. Araujo13Monique Van Hoek14Barbara Birkaya15Virginia Espina16Richard A. Hoefer17Lance Liotta18Alessandra Luchini19Center for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USACenter for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USACenter for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USACenter for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USACenter for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USACenter for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USA; Clinical Laboratory Department, College of Applied Medical Science, King Saud bin Abdulaziz University for Health Sciences, Al Ahsa, Saudi Arabia; King Abdullah International Medical Research Center, Al Ahsa, Saudi ArabiaCenter for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USASchool of Systems Biology, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USASchool of Systems Biology, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USA; College of Applied Medical Sciences, King Saud Bin Abdulaziz University for Health Sciences, Al Ahsa, Saudi ArabiaCenter for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USACenter for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USACollege Dean’s Office, American University, 4400 Massachusetts Avenue, Washington, DC 20016, USACenter for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USAThe University of Melbourne, Grattan Street, Parkville, VIC 3010, AustraliaSchool of Systems Biology, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USACenter for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USACenter for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USADorothy G. Hoefer Comprehensive Breast Center, 11803 Jefferson Avenue, Suite 130, Newport News, VA 23606, USACenter for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USACenter for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MSN 1A9, Manassas, VA 20110, USA; Corresponding authorSummary: Extracellular calcium export by the breast ductal epithelium is crucial during lactation and plays a significant role in breast cancer progression. Intraductal calcium deposition is a hallmark of aggressive premalignant lesions. This study tested the hypothesis that microbiome-derived extracellular vesicles (EVs) influence calcium modulation in premalignant breast cancer lesions. Based on the analysis of plasma, serum, saliva, and tissue collected from breast cancer patients and controls (N = 150), Lactobacillus rhamnosus (Lr) was chosen as the model microbiota. In a BT-474 human breast cancer cell line monolayer culture under acute calcium stress, Lr EVs enhanced intracellular calcium intake. In a BT474 3D spheroid model under chronic calcium stress, Lr EVs increased extracellular calcium deposition and mRNA expression of calcium export channel plasma membrane calcium-transporting ATPase 2 (PMCA2) and stromal interaction molecule 1 (STIM1) in a dose-dependent manner. We propose that Lr EVs influence calcium regulation and mineral deposition, thereby affecting premalignant breast cancer progression.http://www.sciencedirect.com/science/article/pii/S2589004225007990Molecular biologyMicrobiologyCell biologyCancer |
| spellingShingle | Ngoc Vuong Melany Alomia Ahana Byne Purva Gade Thomas Raymond Philipson Rayan Ibrahim Alhammad Cade J. Skislak Intisar Alruwaili Fahad M. Alsaab Weidong Zhou Marissa Howard Andrea Brothers Amanda Haymond Still Robyn P. Araujo Monique Van Hoek Barbara Birkaya Virginia Espina Richard A. Hoefer Lance Liotta Alessandra Luchini Lactobacillus rhamnosus-derived extracellular vesicles influence calcium deposition in a model of breast cancer intraductal calcium stress iScience Molecular biology Microbiology Cell biology Cancer |
| title | Lactobacillus rhamnosus-derived extracellular vesicles influence calcium deposition in a model of breast cancer intraductal calcium stress |
| title_full | Lactobacillus rhamnosus-derived extracellular vesicles influence calcium deposition in a model of breast cancer intraductal calcium stress |
| title_fullStr | Lactobacillus rhamnosus-derived extracellular vesicles influence calcium deposition in a model of breast cancer intraductal calcium stress |
| title_full_unstemmed | Lactobacillus rhamnosus-derived extracellular vesicles influence calcium deposition in a model of breast cancer intraductal calcium stress |
| title_short | Lactobacillus rhamnosus-derived extracellular vesicles influence calcium deposition in a model of breast cancer intraductal calcium stress |
| title_sort | lactobacillus rhamnosus derived extracellular vesicles influence calcium deposition in a model of breast cancer intraductal calcium stress |
| topic | Molecular biology Microbiology Cell biology Cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225007990 |
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