Level of Necrosis in Feline Mammary Tumors: How to Quantify, Why and for What Purpose?
Necrosis is a common finding in human and animal neoplasms. The percentage of tumor necrosis is included in tumor grading schemes in veterinary oncology; however, evaluation methods are often overlooked. Different studies have assessed the prognostic value of tumor necrosis in feline mammary tumors...
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MDPI AG
2024-11-01
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| author | Joana Rodrigues-Jesus Ana Canadas-Sousa Marta Santos Pedro Oliveira Ana Catarina Figueira Carla Marrinhas Gonçalo N. Petrucci Hugo Gregório Flora Tinoco Andrea Goulart Helena Felga Hugo Vilhena Patrícia Dias-Pereira |
| author_facet | Joana Rodrigues-Jesus Ana Canadas-Sousa Marta Santos Pedro Oliveira Ana Catarina Figueira Carla Marrinhas Gonçalo N. Petrucci Hugo Gregório Flora Tinoco Andrea Goulart Helena Felga Hugo Vilhena Patrícia Dias-Pereira |
| author_sort | Joana Rodrigues-Jesus |
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| description | Necrosis is a common finding in human and animal neoplasms. The percentage of tumor necrosis is included in tumor grading schemes in veterinary oncology; however, evaluation methods are often overlooked. Different studies have assessed the prognostic value of tumor necrosis in feline mammary tumors with contradictory results, which could be related to methodologic variability. In this study, a comprehensive evaluation of tumor necrosis in feline mammary tumors (FMTs) was conducted, by applying a semi-quantitative and a quantitative methodology for assessing necrosis. The interobserver agreement, the relationship with clinicopathological characteristics and the prognostic value of necrosis were analyzed in 154 FMT cases. Although subjectivity in the assessment of necrosis existed, an almost perfect agreement (weighted quadratic k = 0.851) between two observers was obtained. Furthermore, there was a significant positive correlation between the semi-quantitative and quantitative methods. Necrosis was more common and more extensive in malignant tumors than in their benign counterparts. Despite the non-significant results in the survival analysis, extensive necrosis was significantly associated with aggressive clinicopathological features, such as higher histological grade, high mitotic count and lymphovascular invasion. Our results support the potential relevance of necrosis in FMT. |
| format | Article |
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| institution | OA Journals |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-75d67db775cc47559892c63debcf28482025-08-20T01:53:52ZengMDPI AGAnimals2076-26152024-11-011422328010.3390/ani14223280Level of Necrosis in Feline Mammary Tumors: How to Quantify, Why and for What Purpose?Joana Rodrigues-Jesus0Ana Canadas-Sousa1Marta Santos2Pedro Oliveira3Ana Catarina Figueira4Carla Marrinhas5Gonçalo N. Petrucci6Hugo Gregório7Flora Tinoco8Andrea Goulart9Helena Felga10Hugo Vilhena11Patrícia Dias-Pereira12Department of Pathology and Molecular Immunology, School of Medicine and Biomedical Sciences, ICBAS-UP, University of Porto, 4050-313 Porto, PortugalDepartment of Pathology and Molecular Immunology, School of Medicine and Biomedical Sciences, ICBAS-UP, University of Porto, 4050-313 Porto, PortugalDepartment of Microscopy, School of Medicine and Biomedical Sciences, ICBAS-UP, University of Porto, 4050-313 Porto, PortugalDepartment of Populations Studies, School of Medicine and Biomedical Sciences, ICBAS-UP, University of Porto, 4050-313 Porto, PortugalCentre for Investigation Vasco da Gama (CIVG), Department of Veterinary Sciences, Vasco da Gama University School, 3020-210 Coimbra, PortugalCentre for Investigation Vasco da Gama (CIVG), Department of Veterinary Sciences, Vasco da Gama University School, 3020-210 Coimbra, PortugalOneVet Veterinary Hospital of Porto (HVP), 4250-475 Porto, PortugalDepartment of Animal and Veterinary Sciences, University Institute for Health Sciences, CESPU, CRL, 4585-116 Gandra, PortugalDra. Flora Tinoco Veterinary Clinic, 4475-498 Maia, PortugalOeste Veterinary Centre, 2410-134 Leiria, PortugalClínica dos Gatos Veterinary Clinic, 4100-207 Porto, PortugalCentre for Investigation Vasco da Gama (CIVG), Department of Veterinary Sciences, Vasco da Gama University School, 3020-210 Coimbra, PortugalDepartment of Pathology and Molecular Immunology, School of Medicine and Biomedical Sciences, ICBAS-UP, University of Porto, 4050-313 Porto, PortugalNecrosis is a common finding in human and animal neoplasms. The percentage of tumor necrosis is included in tumor grading schemes in veterinary oncology; however, evaluation methods are often overlooked. Different studies have assessed the prognostic value of tumor necrosis in feline mammary tumors with contradictory results, which could be related to methodologic variability. In this study, a comprehensive evaluation of tumor necrosis in feline mammary tumors (FMTs) was conducted, by applying a semi-quantitative and a quantitative methodology for assessing necrosis. The interobserver agreement, the relationship with clinicopathological characteristics and the prognostic value of necrosis were analyzed in 154 FMT cases. Although subjectivity in the assessment of necrosis existed, an almost perfect agreement (weighted quadratic k = 0.851) between two observers was obtained. Furthermore, there was a significant positive correlation between the semi-quantitative and quantitative methods. Necrosis was more common and more extensive in malignant tumors than in their benign counterparts. Despite the non-significant results in the survival analysis, extensive necrosis was significantly associated with aggressive clinicopathological features, such as higher histological grade, high mitotic count and lymphovascular invasion. Our results support the potential relevance of necrosis in FMT.https://www.mdpi.com/2076-2615/14/22/3280feline mammary tumorsinterobserver agreementprognosisstereologytumor necrosis |
| spellingShingle | Joana Rodrigues-Jesus Ana Canadas-Sousa Marta Santos Pedro Oliveira Ana Catarina Figueira Carla Marrinhas Gonçalo N. Petrucci Hugo Gregório Flora Tinoco Andrea Goulart Helena Felga Hugo Vilhena Patrícia Dias-Pereira Level of Necrosis in Feline Mammary Tumors: How to Quantify, Why and for What Purpose? Animals feline mammary tumors interobserver agreement prognosis stereology tumor necrosis |
| title | Level of Necrosis in Feline Mammary Tumors: How to Quantify, Why and for What Purpose? |
| title_full | Level of Necrosis in Feline Mammary Tumors: How to Quantify, Why and for What Purpose? |
| title_fullStr | Level of Necrosis in Feline Mammary Tumors: How to Quantify, Why and for What Purpose? |
| title_full_unstemmed | Level of Necrosis in Feline Mammary Tumors: How to Quantify, Why and for What Purpose? |
| title_short | Level of Necrosis in Feline Mammary Tumors: How to Quantify, Why and for What Purpose? |
| title_sort | level of necrosis in feline mammary tumors how to quantify why and for what purpose |
| topic | feline mammary tumors interobserver agreement prognosis stereology tumor necrosis |
| url | https://www.mdpi.com/2076-2615/14/22/3280 |
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