Toll-Like Receptors 2, 4, and 7, Interferon-Gamma, Interleukin 10, and Programmed Death Ligand 1 Transcripts in Leishmanin Skin Test-Positive Reactions of Ibizan Hound Dogs

The leishmanin skin test (LST) is an in vivo technique commonly used to evaluate the Leishmania-specific cellular immune response in dogs. However, information regarding the local immune response in LST-positive reactions is scarce. We examined the pattern of toll-like receptor 2 (TLR2), TLR4, TLR7,...

Full description

Saved in:
Bibliographic Details
Main Authors: Laura Ordeix, Sara Montserrat-Sangrà, Pamela Martínez-Orellana, Laia Solano-Gallego
Format: Article
Language:English
Published: Wiley 2020-01-01
Series:Journal of Immunology Research
Online Access:http://dx.doi.org/10.1155/2020/9602576
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832561152407633920
author Laura Ordeix
Sara Montserrat-Sangrà
Pamela Martínez-Orellana
Laia Solano-Gallego
author_facet Laura Ordeix
Sara Montserrat-Sangrà
Pamela Martínez-Orellana
Laia Solano-Gallego
author_sort Laura Ordeix
collection DOAJ
description The leishmanin skin test (LST) is an in vivo technique commonly used to evaluate the Leishmania-specific cellular immune response in dogs. However, information regarding the local immune response in LST-positive reactions is scarce. We examined the pattern of toll-like receptor 2 (TLR2), TLR4, TLR7, interleukin- (IL-) 10, interferon gamma (IFN-γ), and (program death ligand) PD-L1 gene expression in LST-positive reactions and paired normal-looking skin of nine infected Ibizan hound dogs. Healthy skin from ten seronegative dogs from a nonendemic area was analysed as a negative control. Immune gene expressions were examined by quantitative PCR (qPCR) analysis. LST-positive reactions presented significant upregulation of TLR2, TLR4, IL-10, IFN-γ, and PD-L1 and downregulation of TLR7 when compared with healthy skin of seronegative control dogs from a nonendemic area. All transcripts but TLR7 were significantly higher in LST-positive reaction than in paired normal-looking skin of Ibizan hound. The expression profile of immune genes in LST-positive reactions was similar to that previously observed in clinically lesioned skin of mildly diseased dogs with papular dermatitis due to Leishmania infantum infection. This data provide additional support for the important role of TLRs in canine leishmaniosis.
format Article
id doaj-art-75c5d38a29e54151a24a0d96aba31b69
institution Kabale University
issn 2314-8861
2314-7156
language English
publishDate 2020-01-01
publisher Wiley
record_format Article
series Journal of Immunology Research
spelling doaj-art-75c5d38a29e54151a24a0d96aba31b692025-02-03T01:25:49ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/96025769602576Toll-Like Receptors 2, 4, and 7, Interferon-Gamma, Interleukin 10, and Programmed Death Ligand 1 Transcripts in Leishmanin Skin Test-Positive Reactions of Ibizan Hound DogsLaura Ordeix0Sara Montserrat-Sangrà1Pamela Martínez-Orellana2Laia Solano-Gallego3Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra 08193, SpainDepartament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra 08193, SpainDepartament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra 08193, SpainDepartament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra 08193, SpainThe leishmanin skin test (LST) is an in vivo technique commonly used to evaluate the Leishmania-specific cellular immune response in dogs. However, information regarding the local immune response in LST-positive reactions is scarce. We examined the pattern of toll-like receptor 2 (TLR2), TLR4, TLR7, interleukin- (IL-) 10, interferon gamma (IFN-γ), and (program death ligand) PD-L1 gene expression in LST-positive reactions and paired normal-looking skin of nine infected Ibizan hound dogs. Healthy skin from ten seronegative dogs from a nonendemic area was analysed as a negative control. Immune gene expressions were examined by quantitative PCR (qPCR) analysis. LST-positive reactions presented significant upregulation of TLR2, TLR4, IL-10, IFN-γ, and PD-L1 and downregulation of TLR7 when compared with healthy skin of seronegative control dogs from a nonendemic area. All transcripts but TLR7 were significantly higher in LST-positive reaction than in paired normal-looking skin of Ibizan hound. The expression profile of immune genes in LST-positive reactions was similar to that previously observed in clinically lesioned skin of mildly diseased dogs with papular dermatitis due to Leishmania infantum infection. This data provide additional support for the important role of TLRs in canine leishmaniosis.http://dx.doi.org/10.1155/2020/9602576
spellingShingle Laura Ordeix
Sara Montserrat-Sangrà
Pamela Martínez-Orellana
Laia Solano-Gallego
Toll-Like Receptors 2, 4, and 7, Interferon-Gamma, Interleukin 10, and Programmed Death Ligand 1 Transcripts in Leishmanin Skin Test-Positive Reactions of Ibizan Hound Dogs
Journal of Immunology Research
title Toll-Like Receptors 2, 4, and 7, Interferon-Gamma, Interleukin 10, and Programmed Death Ligand 1 Transcripts in Leishmanin Skin Test-Positive Reactions of Ibizan Hound Dogs
title_full Toll-Like Receptors 2, 4, and 7, Interferon-Gamma, Interleukin 10, and Programmed Death Ligand 1 Transcripts in Leishmanin Skin Test-Positive Reactions of Ibizan Hound Dogs
title_fullStr Toll-Like Receptors 2, 4, and 7, Interferon-Gamma, Interleukin 10, and Programmed Death Ligand 1 Transcripts in Leishmanin Skin Test-Positive Reactions of Ibizan Hound Dogs
title_full_unstemmed Toll-Like Receptors 2, 4, and 7, Interferon-Gamma, Interleukin 10, and Programmed Death Ligand 1 Transcripts in Leishmanin Skin Test-Positive Reactions of Ibizan Hound Dogs
title_short Toll-Like Receptors 2, 4, and 7, Interferon-Gamma, Interleukin 10, and Programmed Death Ligand 1 Transcripts in Leishmanin Skin Test-Positive Reactions of Ibizan Hound Dogs
title_sort toll like receptors 2 4 and 7 interferon gamma interleukin 10 and programmed death ligand 1 transcripts in leishmanin skin test positive reactions of ibizan hound dogs
url http://dx.doi.org/10.1155/2020/9602576
work_keys_str_mv AT lauraordeix tolllikereceptors24and7interferongammainterleukin10andprogrammeddeathligand1transcriptsinleishmaninskintestpositivereactionsofibizanhounddogs
AT saramontserratsangra tolllikereceptors24and7interferongammainterleukin10andprogrammeddeathligand1transcriptsinleishmaninskintestpositivereactionsofibizanhounddogs
AT pamelamartinezorellana tolllikereceptors24and7interferongammainterleukin10andprogrammeddeathligand1transcriptsinleishmaninskintestpositivereactionsofibizanhounddogs
AT laiasolanogallego tolllikereceptors24and7interferongammainterleukin10andprogrammeddeathligand1transcriptsinleishmaninskintestpositivereactionsofibizanhounddogs