EXPERIMENTAL STUDY OF ANTI-THROMBOTIC ACTIVITY OF PENTOXYFILLIN MICROPARTICLES: BASED ON POLY-DL-LACTIDE-CO-GLYCOLIDE IN COMPARISON WITH PENTOXYFILLIN
The aim of the work was a comparative experimental study of the effect of oral administration of Pentoxifylline microparticles based on PLGA, and “standard” Pentoxifylline, on the ADP-induced platelet aggregation process in rats.Materials and methods. Pentoxifylline substance (100 mg/kg) was used as...
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Volgograd State Medical University, Pyatigorsk Medical and Pharmaceutical Institute
2019-05-01
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| Series: | Фармация и фармакология (Пятигорск) |
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| Online Access: | https://www.pharmpharm.ru/jour/article/view/365 |
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| author | T. V. Timchenko V. E. Pogorelyi A. V. Voronkov L. M. Makarova L. I. Scherbakova V. A. Kompantsev A. I. Medvetskyi A. Y. Platonova |
| author_facet | T. V. Timchenko V. E. Pogorelyi A. V. Voronkov L. M. Makarova L. I. Scherbakova V. A. Kompantsev A. I. Medvetskyi A. Y. Platonova |
| author_sort | T. V. Timchenko |
| collection | DOAJ |
| description | The aim of the work was a comparative experimental study of the effect of oral administration of Pentoxifylline microparticles based on PLGA, and “standard” Pentoxifylline, on the ADP-induced platelet aggregation process in rats.Materials and methods. Pentoxifylline substance (100 mg/kg) was used as a reference drug, and PLGA-based Pentoxifylline microparticles with an average dynamic radius of 175.4 nт were used as the object in study. In the experiment, male Wistar rats (m = 300–330 g), the same age group (9 months) were used. They were divided into 3 groups, each of 6 animals. The antiplatelet activity was assessed by determining the degree and rate of platelet aggregation in 1, 3, 5, 8 and 24 hours after a single oral administration of the reference drug and the object under study. Adenosine diphosphate (ADP) at the concentration of 5 μM was used as an aggregation inducer. The aggregation process was recorded using a two-channel laser platelet aggregation analyzer ALAT-2, wavelength of 0.785 μm. by determining the average conventional size of the aggregates.Results. The experiment has proved the following: PLGA-based Pentoxifylline microparticles are more effective at reducing the possibility of platelets to aggregate within 24 hours of the investigation (more than 40%) conventional to the control group value. Besides, it should be noted that according to the effectiveness of the pharmacological action during AD-induced platelet aggregation, the microparticles are commensurate with the standard sample - Pentoxifylline. The action of the microparticle object under study lasts for 24 hours, while the effect of the reference drug is over after 3 hours and then the indicators of the reference group do not differ from those of the control onel.Conclusion. When administered per os, PLGA-based Pentoxifylline microparticles prolong the pharmacological effect significantly – up to 24 hours. |
| format | Article |
| id | doaj-art-75c2ab636c1e439396870842de6063dd |
| institution | DOAJ |
| issn | 2307-9266 2413-2241 |
| language | Russian |
| publishDate | 2019-05-01 |
| publisher | Volgograd State Medical University, Pyatigorsk Medical and Pharmaceutical Institute |
| record_format | Article |
| series | Фармация и фармакология (Пятигорск) |
| spelling | doaj-art-75c2ab636c1e439396870842de6063dd2025-08-20T03:01:04ZrusVolgograd State Medical University, Pyatigorsk Medical and Pharmaceutical InstituteФармация и фармакология (Пятигорск)2307-92662413-22412019-05-01729710410.19163/2307-9266-2019-7-2-97-104301EXPERIMENTAL STUDY OF ANTI-THROMBOTIC ACTIVITY OF PENTOXYFILLIN MICROPARTICLES: BASED ON POLY-DL-LACTIDE-CO-GLYCOLIDE IN COMPARISON WITH PENTOXYFILLINT. V. Timchenko0V. E. Pogorelyi1A. V. Voronkov2L. M. Makarova3L. I. Scherbakova4V. A. Kompantsev5A. I. Medvetskyi6A. Y. Platonova7Pyatigorsk Medical and Pharmaceutical Institute – branch of Volgograd State Medical UniversityPyatigorsk Medical and Pharmaceutical Institute – branch of Volgograd State Medical UniversityPyatigorsk Medical and Pharmaceutical Institute – branch of Volgograd State Medical UniversityPyatigorsk Medical and Pharmaceutical Institute – branch of Volgograd State Medical UniversityPyatigorsk Medical and Pharmaceutical Institute – branch of Volgograd State Medical UniversityPyatigorsk Medical and Pharmaceutical Institute – branch of Volgograd State Medical UniversityPyatigorsk Medical and Pharmaceutical Institute – branch of Volgograd State Medical UniversityPyatigorsk Medical and Pharmaceutical Institute – branch of Volgograd State Medical UniversityThe aim of the work was a comparative experimental study of the effect of oral administration of Pentoxifylline microparticles based on PLGA, and “standard” Pentoxifylline, on the ADP-induced platelet aggregation process in rats.Materials and methods. Pentoxifylline substance (100 mg/kg) was used as a reference drug, and PLGA-based Pentoxifylline microparticles with an average dynamic radius of 175.4 nт were used as the object in study. In the experiment, male Wistar rats (m = 300–330 g), the same age group (9 months) were used. They were divided into 3 groups, each of 6 animals. The antiplatelet activity was assessed by determining the degree and rate of platelet aggregation in 1, 3, 5, 8 and 24 hours after a single oral administration of the reference drug and the object under study. Adenosine diphosphate (ADP) at the concentration of 5 μM was used as an aggregation inducer. The aggregation process was recorded using a two-channel laser platelet aggregation analyzer ALAT-2, wavelength of 0.785 μm. by determining the average conventional size of the aggregates.Results. The experiment has proved the following: PLGA-based Pentoxifylline microparticles are more effective at reducing the possibility of platelets to aggregate within 24 hours of the investigation (more than 40%) conventional to the control group value. Besides, it should be noted that according to the effectiveness of the pharmacological action during AD-induced platelet aggregation, the microparticles are commensurate with the standard sample - Pentoxifylline. The action of the microparticle object under study lasts for 24 hours, while the effect of the reference drug is over after 3 hours and then the indicators of the reference group do not differ from those of the control onel.Conclusion. When administered per os, PLGA-based Pentoxifylline microparticles prolong the pharmacological effect significantly – up to 24 hours.https://www.pharmpharm.ru/jour/article/view/365pentoxifyllinepoly-dl-lactide-co-glycolidepentoxifylline microparticlesrheological properties of bloodantiplatelet agents |
| spellingShingle | T. V. Timchenko V. E. Pogorelyi A. V. Voronkov L. M. Makarova L. I. Scherbakova V. A. Kompantsev A. I. Medvetskyi A. Y. Platonova EXPERIMENTAL STUDY OF ANTI-THROMBOTIC ACTIVITY OF PENTOXYFILLIN MICROPARTICLES: BASED ON POLY-DL-LACTIDE-CO-GLYCOLIDE IN COMPARISON WITH PENTOXYFILLIN Фармация и фармакология (Пятигорск) pentoxifylline poly-dl-lactide-co-glycolide pentoxifylline microparticles rheological properties of blood antiplatelet agents |
| title | EXPERIMENTAL STUDY OF ANTI-THROMBOTIC ACTIVITY OF PENTOXYFILLIN MICROPARTICLES: BASED ON POLY-DL-LACTIDE-CO-GLYCOLIDE IN COMPARISON WITH PENTOXYFILLIN |
| title_full | EXPERIMENTAL STUDY OF ANTI-THROMBOTIC ACTIVITY OF PENTOXYFILLIN MICROPARTICLES: BASED ON POLY-DL-LACTIDE-CO-GLYCOLIDE IN COMPARISON WITH PENTOXYFILLIN |
| title_fullStr | EXPERIMENTAL STUDY OF ANTI-THROMBOTIC ACTIVITY OF PENTOXYFILLIN MICROPARTICLES: BASED ON POLY-DL-LACTIDE-CO-GLYCOLIDE IN COMPARISON WITH PENTOXYFILLIN |
| title_full_unstemmed | EXPERIMENTAL STUDY OF ANTI-THROMBOTIC ACTIVITY OF PENTOXYFILLIN MICROPARTICLES: BASED ON POLY-DL-LACTIDE-CO-GLYCOLIDE IN COMPARISON WITH PENTOXYFILLIN |
| title_short | EXPERIMENTAL STUDY OF ANTI-THROMBOTIC ACTIVITY OF PENTOXYFILLIN MICROPARTICLES: BASED ON POLY-DL-LACTIDE-CO-GLYCOLIDE IN COMPARISON WITH PENTOXYFILLIN |
| title_sort | experimental study of anti thrombotic activity of pentoxyfillin microparticles based on poly dl lactide co glycolide in comparison with pentoxyfillin |
| topic | pentoxifylline poly-dl-lactide-co-glycolide pentoxifylline microparticles rheological properties of blood antiplatelet agents |
| url | https://www.pharmpharm.ru/jour/article/view/365 |
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