Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro
There are promising results in the use of platelet-rich plasma (PRP) for musculoskeletal tissue repair. However, the variability in the methodology for its obtaining may cause different and opposing findings in the literature. Particularly, the choice of the anticoagulant is the first definition to...
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2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/7414036 |
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| author | Ronaldo José Farias Corrêa do Amaral Nemias Pereira da Silva Natália Ferreira Haddad Luana Siqueira Lopes Fábio Dias Ferreira Ricardo Bastos Filho Paola Alejandra Cappelletti Wallace de Mello Eric Cordeiro-Spinetti Alex Balduino |
| author_facet | Ronaldo José Farias Corrêa do Amaral Nemias Pereira da Silva Natália Ferreira Haddad Luana Siqueira Lopes Fábio Dias Ferreira Ricardo Bastos Filho Paola Alejandra Cappelletti Wallace de Mello Eric Cordeiro-Spinetti Alex Balduino |
| author_sort | Ronaldo José Farias Corrêa do Amaral |
| collection | DOAJ |
| description | There are promising results in the use of platelet-rich plasma (PRP) for musculoskeletal tissue repair. However, the variability in the methodology for its obtaining may cause different and opposing findings in the literature. Particularly, the choice of the anticoagulant is the first definition to be made. In this work, blood was collected with sodium citrate (SC), ethylenediaminetetraacetic acid (EDTA), or anticoagulant citrate dextrose (ACD) solution A, as anticoagulants, prior to PRP obtaining. Hematological analysis and growth factors release quantification were performed, and the effects on mesenchymal stromal cell (MSC) culture, such as cytotoxicity and cell proliferation (evaluated by MTT method) and gene expression, were evaluated. The use of EDTA resulted in higher platelet yield in whole blood; however, it induced an increase in the mean platelet volume (MPV) following the blood centrifugation steps for PRP obtaining. The use of SC and ACD resulted in higher induction of MSC proliferation. On the other hand, PRP obtained in SC presented the higher platelet recovery after the blood first centrifugation step and a minimal change in MSC gene expression. Therefore, we suggest the use of SC as the anticoagulant for PRP obtaining. |
| format | Article |
| id | doaj-art-75b5dea3dd0f4141a56ecaae94ee33e5 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-75b5dea3dd0f4141a56ecaae94ee33e52025-08-20T02:06:58ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/74140367414036Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In VitroRonaldo José Farias Corrêa do Amaral0Nemias Pereira da Silva1Natália Ferreira Haddad2Luana Siqueira Lopes3Fábio Dias Ferreira4Ricardo Bastos Filho5Paola Alejandra Cappelletti6Wallace de Mello7Eric Cordeiro-Spinetti8Alex Balduino9Excellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilExcellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilExcellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilExcellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilExcellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilUniversidade Federal Fluminense, 24033-900 Niterói, RJ, BrazilExcellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilExcellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilLaboratório de Biologia e Tecnologia Celular, Universidade Veiga de Almeida, 20270-150 Rio de Janeiro, RJ, BrazilExcellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilThere are promising results in the use of platelet-rich plasma (PRP) for musculoskeletal tissue repair. However, the variability in the methodology for its obtaining may cause different and opposing findings in the literature. Particularly, the choice of the anticoagulant is the first definition to be made. In this work, blood was collected with sodium citrate (SC), ethylenediaminetetraacetic acid (EDTA), or anticoagulant citrate dextrose (ACD) solution A, as anticoagulants, prior to PRP obtaining. Hematological analysis and growth factors release quantification were performed, and the effects on mesenchymal stromal cell (MSC) culture, such as cytotoxicity and cell proliferation (evaluated by MTT method) and gene expression, were evaluated. The use of EDTA resulted in higher platelet yield in whole blood; however, it induced an increase in the mean platelet volume (MPV) following the blood centrifugation steps for PRP obtaining. The use of SC and ACD resulted in higher induction of MSC proliferation. On the other hand, PRP obtained in SC presented the higher platelet recovery after the blood first centrifugation step and a minimal change in MSC gene expression. Therefore, we suggest the use of SC as the anticoagulant for PRP obtaining.http://dx.doi.org/10.1155/2016/7414036 |
| spellingShingle | Ronaldo José Farias Corrêa do Amaral Nemias Pereira da Silva Natália Ferreira Haddad Luana Siqueira Lopes Fábio Dias Ferreira Ricardo Bastos Filho Paola Alejandra Cappelletti Wallace de Mello Eric Cordeiro-Spinetti Alex Balduino Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro Stem Cells International |
| title | Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro |
| title_full | Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro |
| title_fullStr | Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro |
| title_full_unstemmed | Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro |
| title_short | Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro |
| title_sort | platelet rich plasma obtained with different anticoagulants and their effect on platelet numbers and mesenchymal stromal cells behavior in vitro |
| url | http://dx.doi.org/10.1155/2016/7414036 |
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