Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro

There are promising results in the use of platelet-rich plasma (PRP) for musculoskeletal tissue repair. However, the variability in the methodology for its obtaining may cause different and opposing findings in the literature. Particularly, the choice of the anticoagulant is the first definition to...

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Main Authors: Ronaldo José Farias Corrêa do Amaral, Nemias Pereira da Silva, Natália Ferreira Haddad, Luana Siqueira Lopes, Fábio Dias Ferreira, Ricardo Bastos Filho, Paola Alejandra Cappelletti, Wallace de Mello, Eric Cordeiro-Spinetti, Alex Balduino
Format: Article
Language:English
Published: Wiley 2016-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2016/7414036
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author Ronaldo José Farias Corrêa do Amaral
Nemias Pereira da Silva
Natália Ferreira Haddad
Luana Siqueira Lopes
Fábio Dias Ferreira
Ricardo Bastos Filho
Paola Alejandra Cappelletti
Wallace de Mello
Eric Cordeiro-Spinetti
Alex Balduino
author_facet Ronaldo José Farias Corrêa do Amaral
Nemias Pereira da Silva
Natália Ferreira Haddad
Luana Siqueira Lopes
Fábio Dias Ferreira
Ricardo Bastos Filho
Paola Alejandra Cappelletti
Wallace de Mello
Eric Cordeiro-Spinetti
Alex Balduino
author_sort Ronaldo José Farias Corrêa do Amaral
collection DOAJ
description There are promising results in the use of platelet-rich plasma (PRP) for musculoskeletal tissue repair. However, the variability in the methodology for its obtaining may cause different and opposing findings in the literature. Particularly, the choice of the anticoagulant is the first definition to be made. In this work, blood was collected with sodium citrate (SC), ethylenediaminetetraacetic acid (EDTA), or anticoagulant citrate dextrose (ACD) solution A, as anticoagulants, prior to PRP obtaining. Hematological analysis and growth factors release quantification were performed, and the effects on mesenchymal stromal cell (MSC) culture, such as cytotoxicity and cell proliferation (evaluated by MTT method) and gene expression, were evaluated. The use of EDTA resulted in higher platelet yield in whole blood; however, it induced an increase in the mean platelet volume (MPV) following the blood centrifugation steps for PRP obtaining. The use of SC and ACD resulted in higher induction of MSC proliferation. On the other hand, PRP obtained in SC presented the higher platelet recovery after the blood first centrifugation step and a minimal change in MSC gene expression. Therefore, we suggest the use of SC as the anticoagulant for PRP obtaining.
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spelling doaj-art-75b5dea3dd0f4141a56ecaae94ee33e52025-08-20T02:06:58ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/74140367414036Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In VitroRonaldo José Farias Corrêa do Amaral0Nemias Pereira da Silva1Natália Ferreira Haddad2Luana Siqueira Lopes3Fábio Dias Ferreira4Ricardo Bastos Filho5Paola Alejandra Cappelletti6Wallace de Mello7Eric Cordeiro-Spinetti8Alex Balduino9Excellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilExcellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilExcellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilExcellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilExcellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilUniversidade Federal Fluminense, 24033-900 Niterói, RJ, BrazilExcellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilExcellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilLaboratório de Biologia e Tecnologia Celular, Universidade Veiga de Almeida, 20270-150 Rio de Janeiro, RJ, BrazilExcellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, BrazilThere are promising results in the use of platelet-rich plasma (PRP) for musculoskeletal tissue repair. However, the variability in the methodology for its obtaining may cause different and opposing findings in the literature. Particularly, the choice of the anticoagulant is the first definition to be made. In this work, blood was collected with sodium citrate (SC), ethylenediaminetetraacetic acid (EDTA), or anticoagulant citrate dextrose (ACD) solution A, as anticoagulants, prior to PRP obtaining. Hematological analysis and growth factors release quantification were performed, and the effects on mesenchymal stromal cell (MSC) culture, such as cytotoxicity and cell proliferation (evaluated by MTT method) and gene expression, were evaluated. The use of EDTA resulted in higher platelet yield in whole blood; however, it induced an increase in the mean platelet volume (MPV) following the blood centrifugation steps for PRP obtaining. The use of SC and ACD resulted in higher induction of MSC proliferation. On the other hand, PRP obtained in SC presented the higher platelet recovery after the blood first centrifugation step and a minimal change in MSC gene expression. Therefore, we suggest the use of SC as the anticoagulant for PRP obtaining.http://dx.doi.org/10.1155/2016/7414036
spellingShingle Ronaldo José Farias Corrêa do Amaral
Nemias Pereira da Silva
Natália Ferreira Haddad
Luana Siqueira Lopes
Fábio Dias Ferreira
Ricardo Bastos Filho
Paola Alejandra Cappelletti
Wallace de Mello
Eric Cordeiro-Spinetti
Alex Balduino
Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro
Stem Cells International
title Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro
title_full Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro
title_fullStr Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro
title_full_unstemmed Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro
title_short Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro
title_sort platelet rich plasma obtained with different anticoagulants and their effect on platelet numbers and mesenchymal stromal cells behavior in vitro
url http://dx.doi.org/10.1155/2016/7414036
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