The influence of hydrogel stiffness on axonal regeneration after spinal cord injury.
The core challenge in spinal cord injury(SCI) treatment is promoting axonal regeneration and forming new neural connections in damaged areas. However, mature CNS neurons have limited regenerative capacity, causing long-term dysfunction. Axonal regeneration involves elongating axons guided by growth...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0325798 |
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| author | Haiyang Deng Jiaqing Zhou Cong Long Ping Luo Zhong Xiang Hao Zeng |
| author_facet | Haiyang Deng Jiaqing Zhou Cong Long Ping Luo Zhong Xiang Hao Zeng |
| author_sort | Haiyang Deng |
| collection | DOAJ |
| description | The core challenge in spinal cord injury(SCI) treatment is promoting axonal regeneration and forming new neural connections in damaged areas. However, mature CNS neurons have limited regenerative capacity, causing long-term dysfunction. Axonal regeneration involves elongating axons guided by growth cones, which sense and respond to external mechanical signals, integrating them into cytoskeleton reconstruction. After injury, growth cones experience altered mechanical forces due to changes in ECM stiffness. However, systematic studies on matrix stiffness's impact on axonal regeneration post-SCI remain insufficient. This study investigates the influence of hydrogel stiffness on axonal regeneration following SCI. Using gelatin methacryloyl (GelMA) hydrogels with varying stiffness levels, we cultured dorsal root ganglia (DRG) neurons in vitro and applied the hydrogels to a complete transection SCI mouse model. Results demonstrated that higher stiffness GelMA (15% w/v) significantly enhanced axonal extension and sensory functional recovery compared to lower stiffness (7.5% w/v). The study highlights the critical role of ECM stiffness in regulating axonal regeneration and suggests that optimizing hydrogel stiffness can promote neural regeneration and functional recovery after SCI. These findings provide valuable insights for developing therapeutic strategies in SCI treatment. |
| format | Article |
| id | doaj-art-75b2ceaccbfd49a8ab88564ecb20f79e |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-75b2ceaccbfd49a8ab88564ecb20f79e2025-08-20T03:27:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01206e032579810.1371/journal.pone.0325798The influence of hydrogel stiffness on axonal regeneration after spinal cord injury.Haiyang DengJiaqing ZhouCong LongPing LuoZhong XiangHao ZengThe core challenge in spinal cord injury(SCI) treatment is promoting axonal regeneration and forming new neural connections in damaged areas. However, mature CNS neurons have limited regenerative capacity, causing long-term dysfunction. Axonal regeneration involves elongating axons guided by growth cones, which sense and respond to external mechanical signals, integrating them into cytoskeleton reconstruction. After injury, growth cones experience altered mechanical forces due to changes in ECM stiffness. However, systematic studies on matrix stiffness's impact on axonal regeneration post-SCI remain insufficient. This study investigates the influence of hydrogel stiffness on axonal regeneration following SCI. Using gelatin methacryloyl (GelMA) hydrogels with varying stiffness levels, we cultured dorsal root ganglia (DRG) neurons in vitro and applied the hydrogels to a complete transection SCI mouse model. Results demonstrated that higher stiffness GelMA (15% w/v) significantly enhanced axonal extension and sensory functional recovery compared to lower stiffness (7.5% w/v). The study highlights the critical role of ECM stiffness in regulating axonal regeneration and suggests that optimizing hydrogel stiffness can promote neural regeneration and functional recovery after SCI. These findings provide valuable insights for developing therapeutic strategies in SCI treatment.https://doi.org/10.1371/journal.pone.0325798 |
| spellingShingle | Haiyang Deng Jiaqing Zhou Cong Long Ping Luo Zhong Xiang Hao Zeng The influence of hydrogel stiffness on axonal regeneration after spinal cord injury. PLoS ONE |
| title | The influence of hydrogel stiffness on axonal regeneration after spinal cord injury. |
| title_full | The influence of hydrogel stiffness on axonal regeneration after spinal cord injury. |
| title_fullStr | The influence of hydrogel stiffness on axonal regeneration after spinal cord injury. |
| title_full_unstemmed | The influence of hydrogel stiffness on axonal regeneration after spinal cord injury. |
| title_short | The influence of hydrogel stiffness on axonal regeneration after spinal cord injury. |
| title_sort | influence of hydrogel stiffness on axonal regeneration after spinal cord injury |
| url | https://doi.org/10.1371/journal.pone.0325798 |
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