Inducible Clindamycin Resistance and its Molecular Characterisation among Isolates of Staphylococcus aureus from A Tertiary Care Centre in South Kerala
A rise in methicillin-resistant S. aureus (MRSA) infections in hospitals and the population has led to an increase in Macrolide–Lincosamide–Streptogramin type B (MLSB) antibiotic resistance. This has led to increased treatment costs, length of hospital stay and morbidity, as therapy has grown increa...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2025-06-01
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| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/inducible-clindamycin-resistance-and-its-molecular-characterisation-among-isolates-of-staphylococcus-aureus-from-a-tertiary-care-centre-in-south-kerala/ |
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| Summary: | A rise in methicillin-resistant S. aureus (MRSA) infections in hospitals and the population has led to an increase in Macrolide–Lincosamide–Streptogramin type B (MLSB) antibiotic resistance. This has led to increased treatment costs, length of hospital stay and morbidity, as therapy has grown increasingly challenging. As a result, it is imperative that S. aureus isolates and their susceptibility patterns especially to clindamycin and erythromycin, be accurately identified and reported. Of the 341 (100%) S. aureus strains that were recovered from various clinical samples, such as blood, pus, urine, and sputum, 267 (78.6%) were MSSA (methicillin-sensitive S. aureus) and 74 (21.4%) were MRSA. iMLSB (inducible MLSB) resistance detected by D-test was present in 42 (12.3%) isolates, out of which 23 (15.6%) were MSSA and 19 (33.9%) were MRSA. Real-time PCR was done for gene detection on all iMLSB positive isolates. The majority of the isolates – 22 (52.4%) showed both ermA and ermC genes; out of which 13 (56.5%) were MSSA, whereas 9 (47.4%) were MRSA. To correctly identify the true phenotypes sensitive to clindamycin and those showing iMLSB resistance, a reasonably priced, D-test (double disc diffusion test) can be included in routine antibiotic susceptibility testing in clinical settings for all erythromycin-resistant staphylococcal isolates. By following this technique, clinicians treating patients with infections brought on by inducible clindamycin-resistant strains will be guided to forego clindamycin from their treatment regimens, helping to prevent therapeutic failures. |
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| ISSN: | 0973-7510 2581-690X |