Skin collagen peptides of Chinese giant salamander (Andrias davidianus) exert anti-inflammatory effects in LPS-induced RAW264.7 cells via the lincRNA-EPS/NF-κB pathway
Food-derived collagen peptides have recently been reported to have immunomodulatory functions. The Chinese giant salamander (CGS) has both edible and medicinal values. In this study, we tried to verify the immunoregulatory function of CGS skin collagen peptides (CGSSCPs) on macrophages and elucidate...
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Taylor & Francis Group
2024-12-01
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| Series: | Food and Agricultural Immunology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/09540105.2024.2434474 |
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| author | Wencong Shang Shuxian Su Qinrong Li Can Li |
| author_facet | Wencong Shang Shuxian Su Qinrong Li Can Li |
| author_sort | Wencong Shang |
| collection | DOAJ |
| description | Food-derived collagen peptides have recently been reported to have immunomodulatory functions. The Chinese giant salamander (CGS) has both edible and medicinal values. In this study, we tried to verify the immunoregulatory function of CGS skin collagen peptides (CGSSCPs) on macrophages and elucidate its mechanism. CGSSCPs decreased the secretion of nitric oxide (NO) and downregulated the expression of interleukin-6 (IL-6), interleukin-1β (IL-1β), tumour necrosis factor-α (TNF-α), chemokine (C–C motif) ligand 5 (CCL5) and (C-X-C motif) ligand 10 (CXCL10) in Lipopolysaccharide (LPS)-induced RAW264.7 cells. Moreover, CGSSCPs promoted the expression of lincRNA-EPS, and inhibited the phosphorylation of nuclear factor kappa-B (NF-κB) in LPS-induced RAW264.7 cells. Silencing lincRNA-EPS up-regulated the phosphorylation level of NF-κB and the expression of inflammatory cytokines (IL-6, IL-1β, and TNF-α) and chemokines (CCL5 and CXCL10). These suggest that CGSSCPs may regulate RAW264.7 cells by the lincRNA-EPS/NF-κB pathway, which provides a theoretical basis for the development of new anti-inflammatory drugs. |
| format | Article |
| id | doaj-art-759e7faac73b4660a38f71d27393eb4f |
| institution | DOAJ |
| issn | 0954-0105 1465-3443 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj-art-759e7faac73b4660a38f71d27393eb4f2025-08-20T02:52:13ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432024-12-0135110.1080/09540105.2024.2434474Skin collagen peptides of Chinese giant salamander (Andrias davidianus) exert anti-inflammatory effects in LPS-induced RAW264.7 cells via the lincRNA-EPS/NF-κB pathwayWencong Shang0Shuxian Su1Qinrong Li2Can Li3Guiyang University, Guiyang, People’s Republic of ChinaGuiyang University, Guiyang, People’s Republic of ChinaGuiyang University, Guiyang, People’s Republic of ChinaGuiyang University, Guiyang, People’s Republic of ChinaFood-derived collagen peptides have recently been reported to have immunomodulatory functions. The Chinese giant salamander (CGS) has both edible and medicinal values. In this study, we tried to verify the immunoregulatory function of CGS skin collagen peptides (CGSSCPs) on macrophages and elucidate its mechanism. CGSSCPs decreased the secretion of nitric oxide (NO) and downregulated the expression of interleukin-6 (IL-6), interleukin-1β (IL-1β), tumour necrosis factor-α (TNF-α), chemokine (C–C motif) ligand 5 (CCL5) and (C-X-C motif) ligand 10 (CXCL10) in Lipopolysaccharide (LPS)-induced RAW264.7 cells. Moreover, CGSSCPs promoted the expression of lincRNA-EPS, and inhibited the phosphorylation of nuclear factor kappa-B (NF-κB) in LPS-induced RAW264.7 cells. Silencing lincRNA-EPS up-regulated the phosphorylation level of NF-κB and the expression of inflammatory cytokines (IL-6, IL-1β, and TNF-α) and chemokines (CCL5 and CXCL10). These suggest that CGSSCPs may regulate RAW264.7 cells by the lincRNA-EPS/NF-κB pathway, which provides a theoretical basis for the development of new anti-inflammatory drugs.https://www.tandfonline.com/doi/10.1080/09540105.2024.2434474Chinese giant salamanderskin collagen peptideRAW264.7 cellNF-κBlincRNA-EPS |
| spellingShingle | Wencong Shang Shuxian Su Qinrong Li Can Li Skin collagen peptides of Chinese giant salamander (Andrias davidianus) exert anti-inflammatory effects in LPS-induced RAW264.7 cells via the lincRNA-EPS/NF-κB pathway Food and Agricultural Immunology Chinese giant salamander skin collagen peptide RAW264.7 cell NF-κB lincRNA-EPS |
| title | Skin collagen peptides of Chinese giant salamander (Andrias davidianus) exert anti-inflammatory effects in LPS-induced RAW264.7 cells via the lincRNA-EPS/NF-κB pathway |
| title_full | Skin collagen peptides of Chinese giant salamander (Andrias davidianus) exert anti-inflammatory effects in LPS-induced RAW264.7 cells via the lincRNA-EPS/NF-κB pathway |
| title_fullStr | Skin collagen peptides of Chinese giant salamander (Andrias davidianus) exert anti-inflammatory effects in LPS-induced RAW264.7 cells via the lincRNA-EPS/NF-κB pathway |
| title_full_unstemmed | Skin collagen peptides of Chinese giant salamander (Andrias davidianus) exert anti-inflammatory effects in LPS-induced RAW264.7 cells via the lincRNA-EPS/NF-κB pathway |
| title_short | Skin collagen peptides of Chinese giant salamander (Andrias davidianus) exert anti-inflammatory effects in LPS-induced RAW264.7 cells via the lincRNA-EPS/NF-κB pathway |
| title_sort | skin collagen peptides of chinese giant salamander andrias davidianus exert anti inflammatory effects in lps induced raw264 7 cells via the lincrna eps nf κb pathway |
| topic | Chinese giant salamander skin collagen peptide RAW264.7 cell NF-κB lincRNA-EPS |
| url | https://www.tandfonline.com/doi/10.1080/09540105.2024.2434474 |
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