Heterologous expression of Lolium perenne antifreeze protein confers chilling tolerance in tomato
Antifreeze proteins (AFP) are produced by certain plants, animals, fungi and bacteria that enable them to survive upon extremely low temperature. Perennial rye grass, Lolium perenne, was reported to possess AFP which protects them from cold environments. In the present investigation, we isolated AFP...
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KeAi Communications Co., Ltd.
2018-05-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311917617350 |
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| author | Srinivasan Balamurugan Jayan Susan Ann Inchakalody P Varghese Shanmugaraj Bala Murugan Mani Chandra Harish Sarma Rajeev Kumar Ramalingam Sathishkumar |
| author_facet | Srinivasan Balamurugan Jayan Susan Ann Inchakalody P Varghese Shanmugaraj Bala Murugan Mani Chandra Harish Sarma Rajeev Kumar Ramalingam Sathishkumar |
| author_sort | Srinivasan Balamurugan |
| collection | DOAJ |
| description | Antifreeze proteins (AFP) are produced by certain plants, animals, fungi and bacteria that enable them to survive upon extremely low temperature. Perennial rye grass, Lolium perenne, was reported to possess AFP which protects them from cold environments. In the present investigation, we isolated AFP gene from L. perenne and expressed it in tomato plants to elucidate its role upon chilling stress. The T1 transgenic tomato lines were selected and subjected to molecular, biochemical and physiological analyses. Stable integration and transcription of LpAFP in transgenic tomato plants was confirmed by Southern blot hybridization and RT-PCR, respectively. Physiological analyses under chilling conditions showed that the chilling stress induced physiological damage in wild type (WT) plants, while the transgenic plants remained healthy. Total sugar content increased gradually in both WT and transgenic plants throughout the chilling treatment. Interestingly, transgenic plants exhibited remarkable alterations in terms of relative water content (RWC) and electrolyte leakage index (ELI) than those of WT. RWC increased significantly by 3-fold and the electrolyte leakage was reduced by 2.6-fold in transgenic plants comparing with WT. Overall, this report proved that LpAFP gene confers chilling tolerance in transgenic tomato plants and it could be a potential candidate to extrapolate the chilling tolerance on other chilling-sensitive food crops. |
| format | Article |
| id | doaj-art-759da9fa1a674329bbc96a936d482c21 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2018-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-759da9fa1a674329bbc96a936d482c212025-08-20T03:57:08ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192018-05-011751128113610.1016/S2095-3119(17)61735-0Heterologous expression of Lolium perenne antifreeze protein confers chilling tolerance in tomatoSrinivasan Balamurugan0Jayan Susan Ann1Inchakalody P Varghese2Shanmugaraj Bala Murugan3Mani Chandra Harish4Sarma Rajeev Kumar5Ramalingam Sathishkumar6Plant Genetic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore 641046, IndiaPlant Genetic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore 641046, IndiaPlant Genetic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore 641046, IndiaPlant Genetic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore 641046, IndiaPlant Genetic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore 641046, IndiaPlant Genetic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore 641046, IndiaCorrespondence Ramalingam Sathishkumar, Tel: +91-93-60151669; Plant Genetic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore 641046, IndiaAntifreeze proteins (AFP) are produced by certain plants, animals, fungi and bacteria that enable them to survive upon extremely low temperature. Perennial rye grass, Lolium perenne, was reported to possess AFP which protects them from cold environments. In the present investigation, we isolated AFP gene from L. perenne and expressed it in tomato plants to elucidate its role upon chilling stress. The T1 transgenic tomato lines were selected and subjected to molecular, biochemical and physiological analyses. Stable integration and transcription of LpAFP in transgenic tomato plants was confirmed by Southern blot hybridization and RT-PCR, respectively. Physiological analyses under chilling conditions showed that the chilling stress induced physiological damage in wild type (WT) plants, while the transgenic plants remained healthy. Total sugar content increased gradually in both WT and transgenic plants throughout the chilling treatment. Interestingly, transgenic plants exhibited remarkable alterations in terms of relative water content (RWC) and electrolyte leakage index (ELI) than those of WT. RWC increased significantly by 3-fold and the electrolyte leakage was reduced by 2.6-fold in transgenic plants comparing with WT. Overall, this report proved that LpAFP gene confers chilling tolerance in transgenic tomato plants and it could be a potential candidate to extrapolate the chilling tolerance on other chilling-sensitive food crops.http://www.sciencedirect.com/science/article/pii/S2095311917617350Lolium perenne antifreeze proteinchilling tolerancegenetic transformationtransgenic tomato |
| spellingShingle | Srinivasan Balamurugan Jayan Susan Ann Inchakalody P Varghese Shanmugaraj Bala Murugan Mani Chandra Harish Sarma Rajeev Kumar Ramalingam Sathishkumar Heterologous expression of Lolium perenne antifreeze protein confers chilling tolerance in tomato Journal of Integrative Agriculture Lolium perenne antifreeze protein chilling tolerance genetic transformation transgenic tomato |
| title | Heterologous expression of Lolium perenne antifreeze protein confers chilling tolerance in tomato |
| title_full | Heterologous expression of Lolium perenne antifreeze protein confers chilling tolerance in tomato |
| title_fullStr | Heterologous expression of Lolium perenne antifreeze protein confers chilling tolerance in tomato |
| title_full_unstemmed | Heterologous expression of Lolium perenne antifreeze protein confers chilling tolerance in tomato |
| title_short | Heterologous expression of Lolium perenne antifreeze protein confers chilling tolerance in tomato |
| title_sort | heterologous expression of lolium perenne antifreeze protein confers chilling tolerance in tomato |
| topic | Lolium perenne antifreeze protein chilling tolerance genetic transformation transgenic tomato |
| url | http://www.sciencedirect.com/science/article/pii/S2095311917617350 |
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