DNMT1 Gene Expression in Patients with Helicobacter pylori Infection
DNMT1, as a critical enzyme affecting epigenetics through methylation of DNA cytosine-rich sequences, regulates gene expression. Exterior factors including long-term infections, in this study Helicobacter pylori infection, could change host cells’ epigenetics by affecting DNMT1 gene expression. This...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2022/2386891 |
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| author | Manouchehr Ahmadi Hedayati Amjad Ahmadi Zahed Khatooni |
| author_facet | Manouchehr Ahmadi Hedayati Amjad Ahmadi Zahed Khatooni |
| author_sort | Manouchehr Ahmadi Hedayati |
| collection | DOAJ |
| description | DNMT1, as a critical enzyme affecting epigenetics through methylation of DNA cytosine-rich sequences, regulates gene expression. Exterior factors including long-term infections, in this study Helicobacter pylori infection, could change host cells’ epigenetics by affecting DNMT1 gene expression. This study investigated the statistical correlation between H. pylori virulence genes and DNMT1 gene expression in gastric antral epithelial cells of gastric adenocarcinoma and gastritis patients. In a case-control study, 50 and 53 gastritis and gastric adenocarcinoma antral biopsies, including 23 and 21 patients with H. pylori infection, respectively, were collected from hospitals in the west of Iran. Having extracted total RNA from gastric biopsy samples, cDNA was synthesized and virulence genes of H. pylori were detected by using the PCR method. Relative real-time RT PCR was used to detect ΔΔCt fold changes of the DNMT1 gene expression in divided groups of patients based on H. pylori infection and clinical manifestations. The results showed that along with increasing patients’ age, the DNMT1 gene expression will increase in gastric antral epithelial cells of gastric cancer patients (P≤0.05). On the other hand, the biopsy samples with infection of H. pylori cagA, cagY, and cagE genotypes revealed a direct correlation along with increased DNMT1 gene expression. This study revealed the correlations of H. pylori cag pathogenicity island genes with increased DNMT1 gene expression. |
| format | Article |
| id | doaj-art-759820f00a944cdd85e3f94e534b4084 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-759820f00a944cdd85e3f94e534b40842025-08-20T03:35:20ZengWileyThe Scientific World Journal1537-744X2022-01-01202210.1155/2022/2386891DNMT1 Gene Expression in Patients with Helicobacter pylori InfectionManouchehr Ahmadi Hedayati0Amjad Ahmadi1Zahed Khatooni2Liver and Digestive Research CenterDepartment of MicrobiologySchool of PharmacyDNMT1, as a critical enzyme affecting epigenetics through methylation of DNA cytosine-rich sequences, regulates gene expression. Exterior factors including long-term infections, in this study Helicobacter pylori infection, could change host cells’ epigenetics by affecting DNMT1 gene expression. This study investigated the statistical correlation between H. pylori virulence genes and DNMT1 gene expression in gastric antral epithelial cells of gastric adenocarcinoma and gastritis patients. In a case-control study, 50 and 53 gastritis and gastric adenocarcinoma antral biopsies, including 23 and 21 patients with H. pylori infection, respectively, were collected from hospitals in the west of Iran. Having extracted total RNA from gastric biopsy samples, cDNA was synthesized and virulence genes of H. pylori were detected by using the PCR method. Relative real-time RT PCR was used to detect ΔΔCt fold changes of the DNMT1 gene expression in divided groups of patients based on H. pylori infection and clinical manifestations. The results showed that along with increasing patients’ age, the DNMT1 gene expression will increase in gastric antral epithelial cells of gastric cancer patients (P≤0.05). On the other hand, the biopsy samples with infection of H. pylori cagA, cagY, and cagE genotypes revealed a direct correlation along with increased DNMT1 gene expression. This study revealed the correlations of H. pylori cag pathogenicity island genes with increased DNMT1 gene expression.http://dx.doi.org/10.1155/2022/2386891 |
| spellingShingle | Manouchehr Ahmadi Hedayati Amjad Ahmadi Zahed Khatooni DNMT1 Gene Expression in Patients with Helicobacter pylori Infection The Scientific World Journal |
| title | DNMT1 Gene Expression in Patients with Helicobacter pylori Infection |
| title_full | DNMT1 Gene Expression in Patients with Helicobacter pylori Infection |
| title_fullStr | DNMT1 Gene Expression in Patients with Helicobacter pylori Infection |
| title_full_unstemmed | DNMT1 Gene Expression in Patients with Helicobacter pylori Infection |
| title_short | DNMT1 Gene Expression in Patients with Helicobacter pylori Infection |
| title_sort | dnmt1 gene expression in patients with helicobacter pylori infection |
| url | http://dx.doi.org/10.1155/2022/2386891 |
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