In Vitro Antibacterial and Antibiofilm Activity of Lippia alba Essential Oil, Citral, and Carvone against Staphylococcus aureus
In vitro antimicrobial and antibiofilm activities of the Lippia alba essential oil and its major components (citral and carvone) against Staphylococcus aureus were investigated. Essential oils (LA1EO, LA2EO, and LA3EO) were extracted from the aerial parts of three L. alba specimens by hydrodistillat...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2017/4962707 |
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| author | Emanuela Mesquita Porfírio Hider Machado Melo Antônio Matheus Gomes Pereira Theodora Thays Arruda Cavalcante Geovany Amorim Gomes Mário Geraldo de Carvalho Renata Albuquerque Costa Francisco Eduardo Aragão Catunda Júnior |
| author_facet | Emanuela Mesquita Porfírio Hider Machado Melo Antônio Matheus Gomes Pereira Theodora Thays Arruda Cavalcante Geovany Amorim Gomes Mário Geraldo de Carvalho Renata Albuquerque Costa Francisco Eduardo Aragão Catunda Júnior |
| author_sort | Emanuela Mesquita Porfírio |
| collection | DOAJ |
| description | In vitro antimicrobial and antibiofilm activities of the Lippia alba essential oil and its major components (citral and carvone) against Staphylococcus aureus were investigated. Essential oils (LA1EO, LA2EO, and LA3EO) were extracted from the aerial parts of three L. alba specimens by hydrodistillation and analyzed by gas chromatography coupled to a mass spectrometer. Minimum Inhibitory Concentrations (MIC) and Minimum Bacterial Concentration (MBC) were determined by the microdilution method. For the antibiofilm assays, the biomass formation in the biofilm was evaluated by the microtiter-plate technique with the crystal violet (CV) assay and the viability of the bacterial cells was analyzed. All oils and their major components presented antibacterial activity, and the lowest MIC and MBC values were 0.5 mg mL−1 when LA1EO and citral were used. Potential inhibition (100%) of S. aureus biofilm formation at the concentration of 0.5 mg mL−1 of all EOs was observed. However, the elimination of biofilm cells was confirmed at concentrations of 1 mg mL−1, 2 mg mL−1, 2 mg mL−1, and 0.5 mg mL−1 for LA1EO, LA2EO, LA3EO, and citral, respectively. The results obtained in the present research point to the promising antibacterial and antibiofilm potential of L. alba EOs against S. aureus, a species of recognized clinical interest. |
| format | Article |
| id | doaj-art-7593df8b247940868ff10227094c0fa6 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-7593df8b247940868ff10227094c0fa62025-02-03T05:50:32ZengWileyThe Scientific World Journal2356-61401537-744X2017-01-01201710.1155/2017/49627074962707In Vitro Antibacterial and Antibiofilm Activity of Lippia alba Essential Oil, Citral, and Carvone against Staphylococcus aureusEmanuela Mesquita Porfírio0Hider Machado Melo1Antônio Matheus Gomes Pereira2Theodora Thays Arruda Cavalcante3Geovany Amorim Gomes4Mário Geraldo de Carvalho5Renata Albuquerque Costa6Francisco Eduardo Aragão Catunda Júnior7INTA College, Cel. Antonio Rodrigues Magalhães, 359 Sobral, CE, BrazilINTA College, Cel. Antonio Rodrigues Magalhães, 359 Sobral, CE, BrazilINTA College, Cel. Antonio Rodrigues Magalhães, 359 Sobral, CE, BrazilINTA College, Cel. Antonio Rodrigues Magalhães, 359 Sobral, CE, BrazilState University of Acaraú Valley, 62040-370 Sobral, CE, BrazilFederal Rural University of Rio de Janeiro, 23890-000 Seropédica, RJ, BrazilINTA College, Cel. Antonio Rodrigues Magalhães, 359 Sobral, CE, BrazilINTA College, Cel. Antonio Rodrigues Magalhães, 359 Sobral, CE, BrazilIn vitro antimicrobial and antibiofilm activities of the Lippia alba essential oil and its major components (citral and carvone) against Staphylococcus aureus were investigated. Essential oils (LA1EO, LA2EO, and LA3EO) were extracted from the aerial parts of three L. alba specimens by hydrodistillation and analyzed by gas chromatography coupled to a mass spectrometer. Minimum Inhibitory Concentrations (MIC) and Minimum Bacterial Concentration (MBC) were determined by the microdilution method. For the antibiofilm assays, the biomass formation in the biofilm was evaluated by the microtiter-plate technique with the crystal violet (CV) assay and the viability of the bacterial cells was analyzed. All oils and their major components presented antibacterial activity, and the lowest MIC and MBC values were 0.5 mg mL−1 when LA1EO and citral were used. Potential inhibition (100%) of S. aureus biofilm formation at the concentration of 0.5 mg mL−1 of all EOs was observed. However, the elimination of biofilm cells was confirmed at concentrations of 1 mg mL−1, 2 mg mL−1, 2 mg mL−1, and 0.5 mg mL−1 for LA1EO, LA2EO, LA3EO, and citral, respectively. The results obtained in the present research point to the promising antibacterial and antibiofilm potential of L. alba EOs against S. aureus, a species of recognized clinical interest.http://dx.doi.org/10.1155/2017/4962707 |
| spellingShingle | Emanuela Mesquita Porfírio Hider Machado Melo Antônio Matheus Gomes Pereira Theodora Thays Arruda Cavalcante Geovany Amorim Gomes Mário Geraldo de Carvalho Renata Albuquerque Costa Francisco Eduardo Aragão Catunda Júnior In Vitro Antibacterial and Antibiofilm Activity of Lippia alba Essential Oil, Citral, and Carvone against Staphylococcus aureus The Scientific World Journal |
| title | In Vitro Antibacterial and Antibiofilm Activity of Lippia alba Essential Oil, Citral, and Carvone against Staphylococcus aureus |
| title_full | In Vitro Antibacterial and Antibiofilm Activity of Lippia alba Essential Oil, Citral, and Carvone against Staphylococcus aureus |
| title_fullStr | In Vitro Antibacterial and Antibiofilm Activity of Lippia alba Essential Oil, Citral, and Carvone against Staphylococcus aureus |
| title_full_unstemmed | In Vitro Antibacterial and Antibiofilm Activity of Lippia alba Essential Oil, Citral, and Carvone against Staphylococcus aureus |
| title_short | In Vitro Antibacterial and Antibiofilm Activity of Lippia alba Essential Oil, Citral, and Carvone against Staphylococcus aureus |
| title_sort | in vitro antibacterial and antibiofilm activity of lippia alba essential oil citral and carvone against staphylococcus aureus |
| url | http://dx.doi.org/10.1155/2017/4962707 |
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