Evaluation of a Rapid Diagnostic Test (TUBEX-TF) for Diagnosis of Enteric Fever
Background and Aim: Enteric fever is a systemic febrile disease caused by Salmonella enterica serovars Typhi and Paratyphi. Culture provides a conclusive diagnosis of enteric fever. The main difficulties are a longer turnaround time and practicality in a developing nation with a huge population. The...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Archives of Medicine and Health Sciences |
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| Online Access: | https://journals.lww.com/10.4103/amhs.amhs_7_24 |
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| author | Riddhi Singh Nikhil Raj Sangeeta Singh Jyotsna Agarwal Jaya Garg |
| author_facet | Riddhi Singh Nikhil Raj Sangeeta Singh Jyotsna Agarwal Jaya Garg |
| author_sort | Riddhi Singh |
| collection | DOAJ |
| description | Background and Aim:
Enteric fever is a systemic febrile disease caused by Salmonella enterica serovars Typhi and Paratyphi. Culture provides a conclusive diagnosis of enteric fever. The main difficulties are a longer turnaround time and practicality in a developing nation with a huge population. The two RDTs that are now most often used are TUBEX-TF and Enterocheck-WB, where TUBEX-TF can identify IgM antibodies against S. Typhi and S. Paratyphi which are anti-O9 and anti-O2. The aim of this study was to evaluate the performance of the TUBEX-TF test in a typhoid fever endemic area of North India.
Materials and Methods:
In this cross-sectional study, we have performed TUBEX-TF, Enterocheck-WB /Immuno-chromatographic card test, Enzyme linked immunosorbent assay (ELISA), and Widal tests on the sera of 94 clinically suspected cases of enteric fever It was a cross-sectional comparative study that was carried out at a tertiary care institute in North India. In the present study, the ELISA test was taken as a comparative reference test.
Results:
ELISA, TUBEX-TF, Widal, and Immunochromatographic card test were performed on serum samples collected from 94 clinically suspected enteric fever cases, in which 50(53.19%) were positive by ELISA, 45 (47.87%) were positive by TUBEX- TF, 40 (42.5%) were positive by Typhidot and 19 (20.2%) were positive by Widal. For evaluation of TUBEX-TF, sensitivity specificity, diagnostic accuracy, positive predictive and negative predictive values, a comparison with IgM Anti-LPS ELISA test results was performed & it was found 75%,79.55%,80%,74.47%, and 77.17% respectively.
Conclusion:
We conclude that the TUBEX-TF test has a greater sensitivity and specificity than other rapid tests currently on the market. It is a simple, rapid test to diagnose enteric fever. The necessity for large-scale research to evaluate TUBEX-TF is necessary due to interviewer variances so that it can become a novel RDT test for the diagnosis of enteric fever. |
| format | Article |
| id | doaj-art-7592ddd4626e4820b380c4062007a3c2 |
| institution | Kabale University |
| issn | 2321-4848 2321-6085 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Archives of Medicine and Health Sciences |
| spelling | doaj-art-7592ddd4626e4820b380c4062007a3c22025-01-07T05:05:32ZengWolters Kluwer Medknow PublicationsArchives of Medicine and Health Sciences2321-48482321-60852024-12-0112329629910.4103/amhs.amhs_7_24Evaluation of a Rapid Diagnostic Test (TUBEX-TF) for Diagnosis of Enteric FeverRiddhi SinghNikhil RajSangeeta SinghJyotsna AgarwalJaya GargBackground and Aim: Enteric fever is a systemic febrile disease caused by Salmonella enterica serovars Typhi and Paratyphi. Culture provides a conclusive diagnosis of enteric fever. The main difficulties are a longer turnaround time and practicality in a developing nation with a huge population. The two RDTs that are now most often used are TUBEX-TF and Enterocheck-WB, where TUBEX-TF can identify IgM antibodies against S. Typhi and S. Paratyphi which are anti-O9 and anti-O2. The aim of this study was to evaluate the performance of the TUBEX-TF test in a typhoid fever endemic area of North India. Materials and Methods: In this cross-sectional study, we have performed TUBEX-TF, Enterocheck-WB /Immuno-chromatographic card test, Enzyme linked immunosorbent assay (ELISA), and Widal tests on the sera of 94 clinically suspected cases of enteric fever It was a cross-sectional comparative study that was carried out at a tertiary care institute in North India. In the present study, the ELISA test was taken as a comparative reference test. Results: ELISA, TUBEX-TF, Widal, and Immunochromatographic card test were performed on serum samples collected from 94 clinically suspected enteric fever cases, in which 50(53.19%) were positive by ELISA, 45 (47.87%) were positive by TUBEX- TF, 40 (42.5%) were positive by Typhidot and 19 (20.2%) were positive by Widal. For evaluation of TUBEX-TF, sensitivity specificity, diagnostic accuracy, positive predictive and negative predictive values, a comparison with IgM Anti-LPS ELISA test results was performed & it was found 75%,79.55%,80%,74.47%, and 77.17% respectively. Conclusion: We conclude that the TUBEX-TF test has a greater sensitivity and specificity than other rapid tests currently on the market. It is a simple, rapid test to diagnose enteric fever. The necessity for large-scale research to evaluate TUBEX-TF is necessary due to interviewer variances so that it can become a novel RDT test for the diagnosis of enteric fever.https://journals.lww.com/10.4103/amhs.amhs_7_24enteric feverrapid diagnostic testtubextyphoidwidal |
| spellingShingle | Riddhi Singh Nikhil Raj Sangeeta Singh Jyotsna Agarwal Jaya Garg Evaluation of a Rapid Diagnostic Test (TUBEX-TF) for Diagnosis of Enteric Fever Archives of Medicine and Health Sciences enteric fever rapid diagnostic test tubex typhoid widal |
| title | Evaluation of a Rapid Diagnostic Test (TUBEX-TF) for Diagnosis of Enteric Fever |
| title_full | Evaluation of a Rapid Diagnostic Test (TUBEX-TF) for Diagnosis of Enteric Fever |
| title_fullStr | Evaluation of a Rapid Diagnostic Test (TUBEX-TF) for Diagnosis of Enteric Fever |
| title_full_unstemmed | Evaluation of a Rapid Diagnostic Test (TUBEX-TF) for Diagnosis of Enteric Fever |
| title_short | Evaluation of a Rapid Diagnostic Test (TUBEX-TF) for Diagnosis of Enteric Fever |
| title_sort | evaluation of a rapid diagnostic test tubex tf for diagnosis of enteric fever |
| topic | enteric fever rapid diagnostic test tubex typhoid widal |
| url | https://journals.lww.com/10.4103/amhs.amhs_7_24 |
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