ANA IIF Automation: Moving towards Harmonization? Results of a Multicenter Study
Background. Our study aimed to investigate whether the introduction of automated anti-nuclear antibody (ANA) indirect immunofluorescence (IIF) analysis decreases the interlaboratory variability of ANA titer results. Method. Three serum samples were sent to 10 laboratories using the QUANTA-Lyser® in...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/6038137 |
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| _version_ | 1832556475135819776 |
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| author | Stefanie Van den Bremt Sofie Schouwers Marjan Van Blerk Lieve Van Hoovels |
| author_facet | Stefanie Van den Bremt Sofie Schouwers Marjan Van Blerk Lieve Van Hoovels |
| author_sort | Stefanie Van den Bremt |
| collection | DOAJ |
| description | Background. Our study aimed to investigate whether the introduction of automated anti-nuclear antibody (ANA) indirect immunofluorescence (IIF) analysis decreases the interlaboratory variability of ANA titer results. Method. Three serum samples were sent to 10 laboratories using the QUANTA-Lyser® in combination with the NOVA View®. Each laboratory performed the ANA IIF analysis 10x in 1 run and 1x in 10 different runs and determined the endpoint titer by dilution. One of the three samples had been sent in 2012, before the era of ANA IIF automation, by the Belgian National External Quality Assessment (EQA) Scheme. Harmonization was evaluated in terms of variability in fluorescence intensity (LIU) and ANA IIF titer. Results. The evaluation of the intra- and interrun LIU variability revealed a larger variability for 2 laboratories, due to preanalytical and analytical problems. Reanalysis of the EQA sample resulted in a lower titer variability. Diluted endpoint titers were similar to the estimated single well titer and the overall median titer as reported by the EQA in 2012. Conclusion. The introduction of automated microscopic analysis allows more harmonized ANA IIF reporting, provided that this totally automated process is controlled by a thorough quality assurance program, covering the total ANA IIF process. |
| format | Article |
| id | doaj-art-7579c61517414be998d2475352ff6369 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-7579c61517414be998d2475352ff63692025-02-03T05:45:13ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/60381376038137ANA IIF Automation: Moving towards Harmonization? Results of a Multicenter StudyStefanie Van den Bremt0Sofie Schouwers1Marjan Van Blerk2Lieve Van Hoovels3Department of Laboratory Medicine, OLV Hospital, Aalst, BelgiumDepartment of Laboratory Medicine, GZA Hospitals, Antwerp, BelgiumScientific Institute of Public Health (WIV-ISP), Brussels, BelgiumDepartment of Laboratory Medicine, OLV Hospital, Aalst, BelgiumBackground. Our study aimed to investigate whether the introduction of automated anti-nuclear antibody (ANA) indirect immunofluorescence (IIF) analysis decreases the interlaboratory variability of ANA titer results. Method. Three serum samples were sent to 10 laboratories using the QUANTA-Lyser® in combination with the NOVA View®. Each laboratory performed the ANA IIF analysis 10x in 1 run and 1x in 10 different runs and determined the endpoint titer by dilution. One of the three samples had been sent in 2012, before the era of ANA IIF automation, by the Belgian National External Quality Assessment (EQA) Scheme. Harmonization was evaluated in terms of variability in fluorescence intensity (LIU) and ANA IIF titer. Results. The evaluation of the intra- and interrun LIU variability revealed a larger variability for 2 laboratories, due to preanalytical and analytical problems. Reanalysis of the EQA sample resulted in a lower titer variability. Diluted endpoint titers were similar to the estimated single well titer and the overall median titer as reported by the EQA in 2012. Conclusion. The introduction of automated microscopic analysis allows more harmonized ANA IIF reporting, provided that this totally automated process is controlled by a thorough quality assurance program, covering the total ANA IIF process.http://dx.doi.org/10.1155/2017/6038137 |
| spellingShingle | Stefanie Van den Bremt Sofie Schouwers Marjan Van Blerk Lieve Van Hoovels ANA IIF Automation: Moving towards Harmonization? Results of a Multicenter Study Journal of Immunology Research |
| title | ANA IIF Automation: Moving towards Harmonization? Results of a Multicenter Study |
| title_full | ANA IIF Automation: Moving towards Harmonization? Results of a Multicenter Study |
| title_fullStr | ANA IIF Automation: Moving towards Harmonization? Results of a Multicenter Study |
| title_full_unstemmed | ANA IIF Automation: Moving towards Harmonization? Results of a Multicenter Study |
| title_short | ANA IIF Automation: Moving towards Harmonization? Results of a Multicenter Study |
| title_sort | ana iif automation moving towards harmonization results of a multicenter study |
| url | http://dx.doi.org/10.1155/2017/6038137 |
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