Development and Validation of a High‐Performance Liquid Chromatography Method for the Determination of Epoxyaurapten From Pomelo Peel
ABSTRACT Furanocoumarins found in citrus fruits are recognized for their health benefits, but are also known to induce photosensitivity and allergic reactions. Therefore, it is necessary to establish reliable analytical methods for their quantification. In this study, a high‐performance liquid chrom...
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Wiley
2025-08-01
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| Series: | eFood |
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| Online Access: | https://doi.org/10.1002/efd2.70077 |
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| author | Hui Ni Dongfeng Guo Xue Li Lijun Li Yuanfan Yang Xiping Du Yang Hu Shuyu Zhang Feng Chen Fan He |
| author_facet | Hui Ni Dongfeng Guo Xue Li Lijun Li Yuanfan Yang Xiping Du Yang Hu Shuyu Zhang Feng Chen Fan He |
| author_sort | Hui Ni |
| collection | DOAJ |
| description | ABSTRACT Furanocoumarins found in citrus fruits are recognized for their health benefits, but are also known to induce photosensitivity and allergic reactions. Therefore, it is necessary to establish reliable analytical methods for their quantification. In this study, a high‐performance liquid chromatography (HPLC) procedure was developed to quantify epoxyaurapten, a furanocoumarin compound in pomelo peel. The effects of extraction solvent, liquid‐to‐material ratio, ultrasonic time, and extraction cycles on the extraction of epoxyaurapten from pomelo peel were investigated, and the optimal sample pretreatment conditions were determined to ensure the extraction efficiency. The results indicated a high degree of precision with a linear correlation coefficient of 0.9999. The limits of detection and limits of quantification were determined to be 0.5 and 2 μg/mL, respectively. The spiked recovery ranged from 101.24% to 110.46%, with the precision experiment revealing a standard deviation of 0.20% and stability experiments demonstrating a standard deviation of 1.76%. In addition, the HPLC method was successfully employed to detect the content of epoxyaurapten in different pomelo varieties and their various parts. The results revealed that epoxyaurapten was detected only in the exocarp of pomelo cultivars. This finding gives significant implication for further research on the application of epoxyaurapten in pomelo studies. |
| format | Article |
| id | doaj-art-7570a2d6d648414f88af60d0736505eb |
| institution | Kabale University |
| issn | 2666-3066 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | eFood |
| spelling | doaj-art-7570a2d6d648414f88af60d0736505eb2025-08-20T03:36:38ZengWileyeFood2666-30662025-08-0164n/an/a10.1002/efd2.70077Development and Validation of a High‐Performance Liquid Chromatography Method for the Determination of Epoxyaurapten From Pomelo PeelHui Ni0Dongfeng Guo1Xue Li2Lijun Li3Yuanfan Yang4Xiping Du5Yang Hu6Shuyu Zhang7Feng Chen8Fan He9College of Ocean Food and Biological Engineering Jimei University Xiamen ChinaCollege of Ocean Food and Biological Engineering Jimei University Xiamen ChinaCollege of Ocean Food and Biological Engineering Jimei University Xiamen ChinaCollege of Ocean Food and Biological Engineering Jimei University Xiamen ChinaCollege of Ocean Food and Biological Engineering Jimei University Xiamen ChinaCollege of Ocean Food and Biological Engineering Jimei University Xiamen ChinaCollege of Ocean Food and Biological Engineering Jimei University Xiamen ChinaCollege of Ocean Food and Biological Engineering Jimei University Xiamen ChinaDepartment of Food, Nutrition and Packaging Sciences Clemson University Clemson South Carolina USACollege of Ocean Food and Biological Engineering Jimei University Xiamen ChinaABSTRACT Furanocoumarins found in citrus fruits are recognized for their health benefits, but are also known to induce photosensitivity and allergic reactions. Therefore, it is necessary to establish reliable analytical methods for their quantification. In this study, a high‐performance liquid chromatography (HPLC) procedure was developed to quantify epoxyaurapten, a furanocoumarin compound in pomelo peel. The effects of extraction solvent, liquid‐to‐material ratio, ultrasonic time, and extraction cycles on the extraction of epoxyaurapten from pomelo peel were investigated, and the optimal sample pretreatment conditions were determined to ensure the extraction efficiency. The results indicated a high degree of precision with a linear correlation coefficient of 0.9999. The limits of detection and limits of quantification were determined to be 0.5 and 2 μg/mL, respectively. The spiked recovery ranged from 101.24% to 110.46%, with the precision experiment revealing a standard deviation of 0.20% and stability experiments demonstrating a standard deviation of 1.76%. In addition, the HPLC method was successfully employed to detect the content of epoxyaurapten in different pomelo varieties and their various parts. The results revealed that epoxyaurapten was detected only in the exocarp of pomelo cultivars. This finding gives significant implication for further research on the application of epoxyaurapten in pomelo studies.https://doi.org/10.1002/efd2.70077epoxyauraptenfuranocoumarinhigh‐performance liquid chromatographypomelo peel |
| spellingShingle | Hui Ni Dongfeng Guo Xue Li Lijun Li Yuanfan Yang Xiping Du Yang Hu Shuyu Zhang Feng Chen Fan He Development and Validation of a High‐Performance Liquid Chromatography Method for the Determination of Epoxyaurapten From Pomelo Peel eFood epoxyaurapten furanocoumarin high‐performance liquid chromatography pomelo peel |
| title | Development and Validation of a High‐Performance Liquid Chromatography Method for the Determination of Epoxyaurapten From Pomelo Peel |
| title_full | Development and Validation of a High‐Performance Liquid Chromatography Method for the Determination of Epoxyaurapten From Pomelo Peel |
| title_fullStr | Development and Validation of a High‐Performance Liquid Chromatography Method for the Determination of Epoxyaurapten From Pomelo Peel |
| title_full_unstemmed | Development and Validation of a High‐Performance Liquid Chromatography Method for the Determination of Epoxyaurapten From Pomelo Peel |
| title_short | Development and Validation of a High‐Performance Liquid Chromatography Method for the Determination of Epoxyaurapten From Pomelo Peel |
| title_sort | development and validation of a high performance liquid chromatography method for the determination of epoxyaurapten from pomelo peel |
| topic | epoxyaurapten furanocoumarin high‐performance liquid chromatography pomelo peel |
| url | https://doi.org/10.1002/efd2.70077 |
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