Method for determining of cytotoxicity based on the release of fluorescent proteins
Abstract This paper describes a method for determining the cytotoxicity of chemical compounds based on the detection of fluorescent proteins—in this case, green fluorescent protein (GFP) and red fluorescent protein (RFP), which are released into the medium from dead cells. This method is similar in...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | BMC Molecular and Cell Biology |
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| Online Access: | https://doi.org/10.1186/s12860-025-00532-0 |
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| author | Dmitry Lifanov Dulamsuren Zorigt Evgenya Shabalina Abdullah Khalil Konstantin Gorbunov Elena Petersen |
| author_facet | Dmitry Lifanov Dulamsuren Zorigt Evgenya Shabalina Abdullah Khalil Konstantin Gorbunov Elena Petersen |
| author_sort | Dmitry Lifanov |
| collection | DOAJ |
| description | Abstract This paper describes a method for determining the cytotoxicity of chemical compounds based on the detection of fluorescent proteins—in this case, green fluorescent protein (GFP) and red fluorescent protein (RFP), which are released into the medium from dead cells. This method is similar in principle to the lactate dehydrogenase test (LDH test), but it does not require a reaction with a chromogenic substrate. This method also makes it possible to independently determine the viability of different lines when used in cocultures. Experiments were performed on a classical monolayer, spheroids and 3D cultures in alginate hydrogel. Capecitabine was used as a model cytotoxic agent. We included liver cells (Huh7) in a coculture model and determined changes in the cytotoxicity levels of capecitabine against NCI-H1299 cells. The experimental part also found that there were differences in sensitivity to capecitabine depending on the type of 3D cultures used. |
| format | Article |
| id | doaj-art-756aa2dfc3474c2b8f33fc41645be212 |
| institution | Kabale University |
| issn | 2661-8850 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Molecular and Cell Biology |
| spelling | doaj-art-756aa2dfc3474c2b8f33fc41645be2122025-02-02T12:47:20ZengBMCBMC Molecular and Cell Biology2661-88502025-01-0126111210.1186/s12860-025-00532-0Method for determining of cytotoxicity based on the release of fluorescent proteinsDmitry Lifanov0Dulamsuren Zorigt1Evgenya Shabalina2Abdullah Khalil3Konstantin Gorbunov4Elena Petersen5Institute of Future BiophysicsInstitute of Future BiophysicsInstitute of Future BiophysicsInstitute of Future BiophysicsScientific Research Institute for Systems Biology and MedicineInstitute of Future BiophysicsAbstract This paper describes a method for determining the cytotoxicity of chemical compounds based on the detection of fluorescent proteins—in this case, green fluorescent protein (GFP) and red fluorescent protein (RFP), which are released into the medium from dead cells. This method is similar in principle to the lactate dehydrogenase test (LDH test), but it does not require a reaction with a chromogenic substrate. This method also makes it possible to independently determine the viability of different lines when used in cocultures. Experiments were performed on a classical monolayer, spheroids and 3D cultures in alginate hydrogel. Capecitabine was used as a model cytotoxic agent. We included liver cells (Huh7) in a coculture model and determined changes in the cytotoxicity levels of capecitabine against NCI-H1299 cells. The experimental part also found that there were differences in sensitivity to capecitabine depending on the type of 3D cultures used.https://doi.org/10.1186/s12860-025-00532-0Cell culturePharmacologyToxicologyMetabolismSpheroids |
| spellingShingle | Dmitry Lifanov Dulamsuren Zorigt Evgenya Shabalina Abdullah Khalil Konstantin Gorbunov Elena Petersen Method for determining of cytotoxicity based on the release of fluorescent proteins BMC Molecular and Cell Biology Cell culture Pharmacology Toxicology Metabolism Spheroids |
| title | Method for determining of cytotoxicity based on the release of fluorescent proteins |
| title_full | Method for determining of cytotoxicity based on the release of fluorescent proteins |
| title_fullStr | Method for determining of cytotoxicity based on the release of fluorescent proteins |
| title_full_unstemmed | Method for determining of cytotoxicity based on the release of fluorescent proteins |
| title_short | Method for determining of cytotoxicity based on the release of fluorescent proteins |
| title_sort | method for determining of cytotoxicity based on the release of fluorescent proteins |
| topic | Cell culture Pharmacology Toxicology Metabolism Spheroids |
| url | https://doi.org/10.1186/s12860-025-00532-0 |
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