Evaluating the Citotoxicity and Antibacterial Activity of Nano Zinc-Glyde Mixture for Intracanal Irrigation
Objective: In this research, we evaluated the antibacterial efficiency and cytotoxicity of Glyde including its Zinc NPs preparations in three different ratios comparing them with the conventional Glyde. Material and Methods: Antibacterial efficiency was evaluated using inhibition zone method agains...
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University Library System, University of Pittsburgh
2025-08-01
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| Series: | Dentistry 3000 |
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| Online Access: | http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/949 |
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| author | Shammaa Anees Sahib AlAnsari Maitha Sameer Kadhim Hayder Hamed Abed |
| author_facet | Shammaa Anees Sahib AlAnsari Maitha Sameer Kadhim Hayder Hamed Abed |
| author_sort | Shammaa Anees Sahib AlAnsari |
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Objective: In this research, we evaluated the antibacterial efficiency and cytotoxicity of Glyde including its Zinc NPs preparations in three different ratios comparing them with the conventional Glyde. Material and Methods: Antibacterial efficiency was evaluated using inhibition zone method against three common gram-positive pathogens Enterococcus faecali, Lactobacillus and Streptococcus. Three different irrigant preparations were evaluated, GI: Glyde gel alone as a control group, GII: experimental gel (3% Zinc NPs-Glyde), GIII: (4% Zinc NPCs-Glyde gel), GIV: (5% Zinc NPCs-Glyde gel). We evaluated the cytotoxicity by the MTT test. assessing the cells’ viability as soon as possible after 24h, 48h, and 72h. After a color change assessment, spectrophotometric analysis with wavelength ranged from 190-780 nm was performed. The spectrum analysis was performed for diluted mixtures in solvent. Data were plotted and recorded for each wavelength. Kruskal–Wallis (p < 0.05) and post-hoc Bonferroni pairwise (p < 0.05) were used for statistical analysis. Results: All the groups a reduction of the three types of bacteria (p > 0.05) was seen, with inhibition zone increasing with increasing addition of zinc NPs up to 5%. For cytotoxicity, it seems that cell bioavailability remained for 24h, 48h, and declined at 72h. Data were not correlated with addition of zinc nano particles, especially within the visible light range. Conclusion: The addition of zinc nanoparticles has acceptable antibacterial properties and cytotoxic features, and Glyde gel may be used for root canal disinfection without remarkable color change.
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| format | Article |
| id | doaj-art-756a3d57898f44539ca2d388dfc84a20 |
| institution | Kabale University |
| issn | 2167-8677 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | University Library System, University of Pittsburgh |
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| series | Dentistry 3000 |
| spelling | doaj-art-756a3d57898f44539ca2d388dfc84a202025-08-20T03:36:37ZengUniversity Library System, University of PittsburghDentistry 30002167-86772025-08-0113110.5195/d3000.2025.949Evaluating the Citotoxicity and Antibacterial Activity of Nano Zinc-Glyde Mixture for Intracanal IrrigationShammaa Anees Sahib AlAnsari0Maitha Sameer KadhimHayder Hamed AbedDepartment of Conservative Dentistry, College of Dentistery, Ibn Sina University of medical and pharmaceutical Science, Baghdad, Iraq. Objective: In this research, we evaluated the antibacterial efficiency and cytotoxicity of Glyde including its Zinc NPs preparations in three different ratios comparing them with the conventional Glyde. Material and Methods: Antibacterial efficiency was evaluated using inhibition zone method against three common gram-positive pathogens Enterococcus faecali, Lactobacillus and Streptococcus. Three different irrigant preparations were evaluated, GI: Glyde gel alone as a control group, GII: experimental gel (3% Zinc NPs-Glyde), GIII: (4% Zinc NPCs-Glyde gel), GIV: (5% Zinc NPCs-Glyde gel). We evaluated the cytotoxicity by the MTT test. assessing the cells’ viability as soon as possible after 24h, 48h, and 72h. After a color change assessment, spectrophotometric analysis with wavelength ranged from 190-780 nm was performed. The spectrum analysis was performed for diluted mixtures in solvent. Data were plotted and recorded for each wavelength. Kruskal–Wallis (p < 0.05) and post-hoc Bonferroni pairwise (p < 0.05) were used for statistical analysis. Results: All the groups a reduction of the three types of bacteria (p > 0.05) was seen, with inhibition zone increasing with increasing addition of zinc NPs up to 5%. For cytotoxicity, it seems that cell bioavailability remained for 24h, 48h, and declined at 72h. Data were not correlated with addition of zinc nano particles, especially within the visible light range. Conclusion: The addition of zinc nanoparticles has acceptable antibacterial properties and cytotoxic features, and Glyde gel may be used for root canal disinfection without remarkable color change. http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/949IrrigationZincAntibacterialEndodontics |
| spellingShingle | Shammaa Anees Sahib AlAnsari Maitha Sameer Kadhim Hayder Hamed Abed Evaluating the Citotoxicity and Antibacterial Activity of Nano Zinc-Glyde Mixture for Intracanal Irrigation Dentistry 3000 Irrigation Zinc Antibacterial Endodontics |
| title | Evaluating the Citotoxicity and Antibacterial Activity of Nano Zinc-Glyde Mixture for Intracanal Irrigation |
| title_full | Evaluating the Citotoxicity and Antibacterial Activity of Nano Zinc-Glyde Mixture for Intracanal Irrigation |
| title_fullStr | Evaluating the Citotoxicity and Antibacterial Activity of Nano Zinc-Glyde Mixture for Intracanal Irrigation |
| title_full_unstemmed | Evaluating the Citotoxicity and Antibacterial Activity of Nano Zinc-Glyde Mixture for Intracanal Irrigation |
| title_short | Evaluating the Citotoxicity and Antibacterial Activity of Nano Zinc-Glyde Mixture for Intracanal Irrigation |
| title_sort | evaluating the citotoxicity and antibacterial activity of nano zinc glyde mixture for intracanal irrigation |
| topic | Irrigation Zinc Antibacterial Endodontics |
| url | http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/949 |
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