Evaluating the Citotoxicity and Antibacterial Activity of Nano Zinc-Glyde Mixture for Intracanal Irrigation
Objective: In this research, we evaluated the antibacterial efficiency and cytotoxicity of Glyde including its Zinc NPs preparations in three different ratios comparing them with the conventional Glyde. Material and Methods: Antibacterial efficiency was evaluated using inhibition zone method agains...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
University Library System, University of Pittsburgh
2025-08-01
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| Series: | Dentistry 3000 |
| Subjects: | |
| Online Access: | http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/949 |
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| Summary: | Objective: In this research, we evaluated the antibacterial efficiency and cytotoxicity of Glyde including its Zinc NPs preparations in three different ratios comparing them with the conventional Glyde. Material and Methods: Antibacterial efficiency was evaluated using inhibition zone method against three common gram-positive pathogens Enterococcus faecali, Lactobacillus and Streptococcus. Three different irrigant preparations were evaluated, GI: Glyde gel alone as a control group, GII: experimental gel (3% Zinc NPs-Glyde), GIII: (4% Zinc NPCs-Glyde gel), GIV: (5% Zinc NPCs-Glyde gel). We evaluated the cytotoxicity by the MTT test. assessing the cells’ viability as soon as possible after 24h, 48h, and 72h. After a color change assessment, spectrophotometric analysis with wavelength ranged from 190-780 nm was performed. The spectrum analysis was performed for diluted mixtures in solvent. Data were plotted and recorded for each wavelength. Kruskal–Wallis (p < 0.05) and post-hoc Bonferroni pairwise (p < 0.05) were used for statistical analysis. Results: All the groups a reduction of the three types of bacteria (p > 0.05) was seen, with inhibition zone increasing with increasing addition of zinc NPs up to 5%. For cytotoxicity, it seems that cell bioavailability remained for 24h, 48h, and declined at 72h. Data were not correlated with addition of zinc nano particles, especially within the visible light range. Conclusion: The addition of zinc nanoparticles has acceptable antibacterial properties and cytotoxic features, and Glyde gel may be used for root canal disinfection without remarkable color change.
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| ISSN: | 2167-8677 |