Molecular Detection of Urogenital Schistosomiasis in Community Level in Semi-Rural Areas in South-East Gabon
Background: Urogenital schistosomiasis is endemic in Gabon. Our study aimed to detect the prevalence of urinary schistosomiasis and to evaluate the diagnostic performance of the qPCR technique compared to microscopy for the detection of <i>Schistosoma haematobium</i> at the community lev...
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MDPI AG
2025-04-01
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| author | Lady Charlène Kouna Sandrine Lydie Oyegue-Liabagui Chenis Nick Atiga Chérone Nancy Mbani Mpega Ntigui Roméo Karl Imboumy-Limoukou Jean Claude Biteghe BI Essone Steede Seinnat Ontoua Diamella Nancy Moukodoum Alain Prince Okouga Jean Bernard Lekana-Douki |
| author_facet | Lady Charlène Kouna Sandrine Lydie Oyegue-Liabagui Chenis Nick Atiga Chérone Nancy Mbani Mpega Ntigui Roméo Karl Imboumy-Limoukou Jean Claude Biteghe BI Essone Steede Seinnat Ontoua Diamella Nancy Moukodoum Alain Prince Okouga Jean Bernard Lekana-Douki |
| author_sort | Lady Charlène Kouna |
| collection | DOAJ |
| description | Background: Urogenital schistosomiasis is endemic in Gabon. Our study aimed to detect the prevalence of urinary schistosomiasis and to evaluate the diagnostic performance of the qPCR technique compared to microscopy for the detection of <i>Schistosoma haematobium</i> at the community level in a semi-rural area. Method: A cross-sectional survey was carried out. Urine samples were examined using Urine TICK test strips, a filtration technique, and qPCR. <i>Schistosoma haematobium</i> was detected by targeting the Dra1 gene. Results: The prevalence of urogenital schistosomiasis was determined and the performance of real-time PCR and urine strips was compared with that of urinary filtration. A total of 281 participants were enrolled in the study. The prevalence of urogenital schistosomiasis was increased slightly with the molecular technique (40.9%) compared to microscopy (36.7%), and the hematuria rate with Urine STICK was 33.5%. SAC (5–14 years old), Pre-SAC (>5 years old), and adolescents (15–17 years old) were the most affected group according to, respectively, whatever method was used. qPCR showed good agreement with microscopy, as well as excellent sensitivity (99.03%) and specificity (93.3). There was a good correlation between the number of eggs per 10 mL and the cycle threshold range. Conclusion: These results show the importance of using a combination of diagnostic tools in the surveillance of schistosomiasis, particularly in preschool children, adolescents, women of childbearing age, and chronic and asymptomatic cases. |
| format | Article |
| id | doaj-art-7568a9aedfb946038bc823def078bc2c |
| institution | DOAJ |
| issn | 2075-4418 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Diagnostics |
| spelling | doaj-art-7568a9aedfb946038bc823def078bc2c2025-08-20T02:58:47ZengMDPI AGDiagnostics2075-44182025-04-01159105210.3390/diagnostics15091052Molecular Detection of Urogenital Schistosomiasis in Community Level in Semi-Rural Areas in South-East GabonLady Charlène Kouna0Sandrine Lydie Oyegue-Liabagui1Chenis Nick Atiga2Chérone Nancy Mbani Mpega Ntigui3Roméo Karl Imboumy-Limoukou4Jean Claude Biteghe BI Essone5Steede Seinnat Ontoua6Diamella Nancy Moukodoum7Alain Prince Okouga8Jean Bernard Lekana-Douki9Unité d’Evolution, Epidémiologie et Résistances Parasitaires (UNEEREP), Centre Interdisciplinaire de Recherche Médicales de Franceville, Franceville BP 769, GabonUnité d’Evolution, Epidémiologie et Résistances Parasitaires (UNEEREP), Centre Interdisciplinaire de Recherche Médicales de Franceville, Franceville BP 769, GabonUnité d’Evolution, Epidémiologie et Résistances Parasitaires (UNEEREP), Centre Interdisciplinaire de Recherche Médicales de Franceville, Franceville BP 769, GabonUnité d’Evolution, Epidémiologie et Résistances Parasitaires (UNEEREP), Centre Interdisciplinaire de Recherche Médicales de Franceville, Franceville BP 769, GabonUnité d’Evolution, Epidémiologie et Résistances Parasitaires (UNEEREP), Centre Interdisciplinaire de Recherche Médicales de Franceville, Franceville BP 769, GabonUnité d’Evolution, Epidémiologie et Résistances Parasitaires (UNEEREP), Centre Interdisciplinaire de Recherche Médicales de Franceville, Franceville BP 769, GabonUnité d’Evolution, Epidémiologie et Résistances Parasitaires (UNEEREP), Centre Interdisciplinaire de Recherche Médicales de Franceville, Franceville BP 769, GabonUnité d’Evolution, Epidémiologie et Résistances Parasitaires (UNEEREP), Centre Interdisciplinaire de Recherche Médicales de Franceville, Franceville BP 769, GabonUnité d’Evolution, Epidémiologie et Résistances Parasitaires (UNEEREP), Centre Interdisciplinaire de Recherche Médicales de Franceville, Franceville BP 769, GabonUnité d’Evolution, Epidémiologie et Résistances Parasitaires (UNEEREP), Centre Interdisciplinaire de Recherche Médicales de Franceville, Franceville BP 769, GabonBackground: Urogenital schistosomiasis is endemic in Gabon. Our study aimed to detect the prevalence of urinary schistosomiasis and to evaluate the diagnostic performance of the qPCR technique compared to microscopy for the detection of <i>Schistosoma haematobium</i> at the community level in a semi-rural area. Method: A cross-sectional survey was carried out. Urine samples were examined using Urine TICK test strips, a filtration technique, and qPCR. <i>Schistosoma haematobium</i> was detected by targeting the Dra1 gene. Results: The prevalence of urogenital schistosomiasis was determined and the performance of real-time PCR and urine strips was compared with that of urinary filtration. A total of 281 participants were enrolled in the study. The prevalence of urogenital schistosomiasis was increased slightly with the molecular technique (40.9%) compared to microscopy (36.7%), and the hematuria rate with Urine STICK was 33.5%. SAC (5–14 years old), Pre-SAC (>5 years old), and adolescents (15–17 years old) were the most affected group according to, respectively, whatever method was used. qPCR showed good agreement with microscopy, as well as excellent sensitivity (99.03%) and specificity (93.3). There was a good correlation between the number of eggs per 10 mL and the cycle threshold range. Conclusion: These results show the importance of using a combination of diagnostic tools in the surveillance of schistosomiasis, particularly in preschool children, adolescents, women of childbearing age, and chronic and asymptomatic cases.https://www.mdpi.com/2075-4418/15/9/1052<i>Schistosoma haematobium</i>urinaryqPCRurine stickfiltration |
| spellingShingle | Lady Charlène Kouna Sandrine Lydie Oyegue-Liabagui Chenis Nick Atiga Chérone Nancy Mbani Mpega Ntigui Roméo Karl Imboumy-Limoukou Jean Claude Biteghe BI Essone Steede Seinnat Ontoua Diamella Nancy Moukodoum Alain Prince Okouga Jean Bernard Lekana-Douki Molecular Detection of Urogenital Schistosomiasis in Community Level in Semi-Rural Areas in South-East Gabon Diagnostics <i>Schistosoma haematobium</i> urinary qPCR urine stick filtration |
| title | Molecular Detection of Urogenital Schistosomiasis in Community Level in Semi-Rural Areas in South-East Gabon |
| title_full | Molecular Detection of Urogenital Schistosomiasis in Community Level in Semi-Rural Areas in South-East Gabon |
| title_fullStr | Molecular Detection of Urogenital Schistosomiasis in Community Level in Semi-Rural Areas in South-East Gabon |
| title_full_unstemmed | Molecular Detection of Urogenital Schistosomiasis in Community Level in Semi-Rural Areas in South-East Gabon |
| title_short | Molecular Detection of Urogenital Schistosomiasis in Community Level in Semi-Rural Areas in South-East Gabon |
| title_sort | molecular detection of urogenital schistosomiasis in community level in semi rural areas in south east gabon |
| topic | <i>Schistosoma haematobium</i> urinary qPCR urine stick filtration |
| url | https://www.mdpi.com/2075-4418/15/9/1052 |
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