In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood
A diversion of raw material from wood to bamboo is necessary. In vitro culture of bamboo can be used to provide a high number of seedling. The aim of this study was to increase the multiplication of a high quality Bambusa balcooa as a wood alternative material. Part of plants used was the sterile ax...
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Universitas Negeri Semarang
2018-04-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
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| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/11079 |
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| author | Siti Nurhayani Rita Megia Ragapadmi Purnamaningsih |
| author_facet | Siti Nurhayani Rita Megia Ragapadmi Purnamaningsih |
| author_sort | Siti Nurhayani |
| collection | DOAJ |
| description | A diversion of raw material from wood to bamboo is necessary. In vitro culture of bamboo can be used to provide a high number of seedling. The aim of this study was to increase the multiplication of a high quality Bambusa balcooa as a wood alternative material. Part of plants used was the sterile axillary shoot. The explants were planted on MS0 medium for 2 weeks and later on multiplication medium MS+0.3 mg/l BAP + 0.3mg/l TDZ. The shoots obtained were fragmented into clusters (3-5 shoots) used for the next multiplication stage using five different medium formulas: (1) MS0; MS containing: (2) 0.1 mg/l BAP, (3) 0.3 mg/l BAP, (4) 0.1 mg/l BAP + 0.1 mg/l TDZ and (5) 0.3 mg/l BAP + 0.1 mg/l TDZ. The results showed that MS medium containing 0.1 mg/l BAP + 0.1mg/l TDZ was the best medium for B. balcooa propagation. The shoots produced from aforementioned medium had a better quality compared to the other medium. Forty days after planting, the average number of shoots in this medium was 14.25. MS medium containing 0.3 mg/l BAP + 0.1 mg/l TDZ produced the highest number of shoot but in lower quality. Rooting medium containing 10 mg/l IBA + 5 mg/l NAA produced 9-16 root in 8 weeks. Vermicompost was more prevalent for the acclimatization of B. balcooa compared to compost. The use of B.balcooa resulted in in vitro propagation as a substitute alternative for wood is expected to save the environment from illegal logging. |
| format | Article |
| id | doaj-art-756836d23bcf474c9ebaae0d64080c5e |
| institution | Kabale University |
| issn | 2085-191X 2338-7610 |
| language | English |
| publishDate | 2018-04-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj-art-756836d23bcf474c9ebaae0d64080c5e2025-08-20T03:35:32ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102018-04-0110119820410.15294/biosaintifika.v10i1.110797351In Vitro Propagation of Bambusa balcooa as Alternative Material of WoodSiti Nurhayani0Rita Megia1Ragapadmi Purnamaningsih2Plant Biology Graduate Program, Institut Pertanian BogorDepartement of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian BogorIndonesian Center for Agricultural Biotechnology and Genetic Resources Research and DevelopmentA diversion of raw material from wood to bamboo is necessary. In vitro culture of bamboo can be used to provide a high number of seedling. The aim of this study was to increase the multiplication of a high quality Bambusa balcooa as a wood alternative material. Part of plants used was the sterile axillary shoot. The explants were planted on MS0 medium for 2 weeks and later on multiplication medium MS+0.3 mg/l BAP + 0.3mg/l TDZ. The shoots obtained were fragmented into clusters (3-5 shoots) used for the next multiplication stage using five different medium formulas: (1) MS0; MS containing: (2) 0.1 mg/l BAP, (3) 0.3 mg/l BAP, (4) 0.1 mg/l BAP + 0.1 mg/l TDZ and (5) 0.3 mg/l BAP + 0.1 mg/l TDZ. The results showed that MS medium containing 0.1 mg/l BAP + 0.1mg/l TDZ was the best medium for B. balcooa propagation. The shoots produced from aforementioned medium had a better quality compared to the other medium. Forty days after planting, the average number of shoots in this medium was 14.25. MS medium containing 0.3 mg/l BAP + 0.1 mg/l TDZ produced the highest number of shoot but in lower quality. Rooting medium containing 10 mg/l IBA + 5 mg/l NAA produced 9-16 root in 8 weeks. Vermicompost was more prevalent for the acclimatization of B. balcooa compared to compost. The use of B.balcooa resulted in in vitro propagation as a substitute alternative for wood is expected to save the environment from illegal logging.https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/11079b. balcooamicropropagationwood alternative |
| spellingShingle | Siti Nurhayani Rita Megia Ragapadmi Purnamaningsih In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood Biosaintifika: Journal of Biology & Biology Education b. balcooa micropropagation wood alternative |
| title | In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood |
| title_full | In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood |
| title_fullStr | In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood |
| title_full_unstemmed | In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood |
| title_short | In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood |
| title_sort | in vitro propagation of bambusa balcooa as alternative material of wood |
| topic | b. balcooa micropropagation wood alternative |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/11079 |
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