Modulating ultrasound-assisted extraction of polysaccharide-protein complexes from β-glucan-rich medicinal mushrooms for enhanced antioxidant activity
The growing nutraceutical interest in polysaccharide-protein complexes (PSPs) from β-glucan-rich medicinal mushrooms necessitates efficient extraction methods. This study examined the impact of various ultrasound-assisted extraction (UAE) parameters, including ultrasound power, temperature, mushroom...
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Elsevier
2025-06-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893925001550 |
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| author | Emily Wan Ting Tam Yi Ching Cheung Wesley Chin Ho Lung Ching Ching Hui William W.Y. Lam Stella Siu Mui Ng Franklin Wang Ngai Chow |
| author_facet | Emily Wan Ting Tam Yi Ching Cheung Wesley Chin Ho Lung Ching Ching Hui William W.Y. Lam Stella Siu Mui Ng Franklin Wang Ngai Chow |
| author_sort | Emily Wan Ting Tam |
| collection | DOAJ |
| description | The growing nutraceutical interest in polysaccharide-protein complexes (PSPs) from β-glucan-rich medicinal mushrooms necessitates efficient extraction methods. This study examined the impact of various ultrasound-assisted extraction (UAE) parameters, including ultrasound power, temperature, mushroom particle size, and solid-to-liquid ratio, on the composition and antioxidant activities of PSPs extracts from Lentinula edodes (L. edodes) and Grifola frondosa (G. frondosa). Using a Box–Behnken design and response surface methodology, second-order polynomial models were developed to correlate UAE parameters with PSPs antioxidant activity, demonstrating strong model fit with high F-values and low p-values (F-value = 8.64, p-value = 0.0143 for L. edodes; F-value = 42.41, p-value = 0.0003 for G. frondosa). The models showed a high degree of correlation between observed and predicted values, with variations depending on mushroom species. Optimal UAE conditions for achieving the highest antioxidant PSPs were identified for L. edodes (55 °C, 6.21 W/cm² ultrasound power, particle size <600 µm) and G. frondosa (70 °C, 6.21 W/cm² ultrasound power, particle size of 600–850 µm). A higher protein-to-polysaccharide ratio in PSPs was correlated with enhanced antioxidant activity. UAE presents a promising technique for enhancing the antioxidant properties of PSPs in pharmaceutical formulations, potentially broadening their use in extracting bioactive compounds from other medicinal mushrooms to promote health and wellness. |
| format | Article |
| id | doaj-art-7560165a13d34cfda19eb518188bbbdd |
| institution | OA Journals |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-7560165a13d34cfda19eb518188bbbdd2025-08-20T02:07:39ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-06-011010081710.1016/j.carpta.2025.100817Modulating ultrasound-assisted extraction of polysaccharide-protein complexes from β-glucan-rich medicinal mushrooms for enhanced antioxidant activityEmily Wan Ting Tam0Yi Ching Cheung1Wesley Chin Ho Lung2Ching Ching Hui3William W.Y. Lam4Stella Siu Mui Ng5Franklin Wang Ngai Chow6Department of Applied Science, Hong Kong Metropolitan University, Ho Man Tin, Kowloon, Hong Kong SAR, China; Corresponding author.Department of Food and Health Sciences, Technological and Higher Education Institute of Hong Kong, Hong Kong SAR, ChinaDepartment of Applied Science, Hong Kong Metropolitan University, Ho Man Tin, Kowloon, Hong Kong SAR, ChinaDepartment of Applied Science, Hong Kong Metropolitan University, Ho Man Tin, Kowloon, Hong Kong SAR, ChinaDepartment of Food and Health Sciences, Technological and Higher Education Institute of Hong Kong, Hong Kong SAR, ChinaDepartment of Applied Science, Hong Kong Metropolitan University, Ho Man Tin, Kowloon, Hong Kong SAR, ChinaDepartment of Health Technology and Informatics, The Hong Kong Polytechnic University, Kowloon, Hong Kong SAR, China; Research Centre for Chinese Medicine Innovation, The Hong Kong Polytechnic University, Hong Kong, Hong Kong SAR, China; Corresponding author at : Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Kowloon, Hong Kong SAR, China.The growing nutraceutical interest in polysaccharide-protein complexes (PSPs) from β-glucan-rich medicinal mushrooms necessitates efficient extraction methods. This study examined the impact of various ultrasound-assisted extraction (UAE) parameters, including ultrasound power, temperature, mushroom particle size, and solid-to-liquid ratio, on the composition and antioxidant activities of PSPs extracts from Lentinula edodes (L. edodes) and Grifola frondosa (G. frondosa). Using a Box–Behnken design and response surface methodology, second-order polynomial models were developed to correlate UAE parameters with PSPs antioxidant activity, demonstrating strong model fit with high F-values and low p-values (F-value = 8.64, p-value = 0.0143 for L. edodes; F-value = 42.41, p-value = 0.0003 for G. frondosa). The models showed a high degree of correlation between observed and predicted values, with variations depending on mushroom species. Optimal UAE conditions for achieving the highest antioxidant PSPs were identified for L. edodes (55 °C, 6.21 W/cm² ultrasound power, particle size <600 µm) and G. frondosa (70 °C, 6.21 W/cm² ultrasound power, particle size of 600–850 µm). A higher protein-to-polysaccharide ratio in PSPs was correlated with enhanced antioxidant activity. UAE presents a promising technique for enhancing the antioxidant properties of PSPs in pharmaceutical formulations, potentially broadening their use in extracting bioactive compounds from other medicinal mushrooms to promote health and wellness.http://www.sciencedirect.com/science/article/pii/S2666893925001550Ultrasound-assisted extractionPolysaccharide-protein complexesMedicinal mushroomAntioxidant activityResponse surface methodologyβ-glucan |
| spellingShingle | Emily Wan Ting Tam Yi Ching Cheung Wesley Chin Ho Lung Ching Ching Hui William W.Y. Lam Stella Siu Mui Ng Franklin Wang Ngai Chow Modulating ultrasound-assisted extraction of polysaccharide-protein complexes from β-glucan-rich medicinal mushrooms for enhanced antioxidant activity Carbohydrate Polymer Technologies and Applications Ultrasound-assisted extraction Polysaccharide-protein complexes Medicinal mushroom Antioxidant activity Response surface methodology β-glucan |
| title | Modulating ultrasound-assisted extraction of polysaccharide-protein complexes from β-glucan-rich medicinal mushrooms for enhanced antioxidant activity |
| title_full | Modulating ultrasound-assisted extraction of polysaccharide-protein complexes from β-glucan-rich medicinal mushrooms for enhanced antioxidant activity |
| title_fullStr | Modulating ultrasound-assisted extraction of polysaccharide-protein complexes from β-glucan-rich medicinal mushrooms for enhanced antioxidant activity |
| title_full_unstemmed | Modulating ultrasound-assisted extraction of polysaccharide-protein complexes from β-glucan-rich medicinal mushrooms for enhanced antioxidant activity |
| title_short | Modulating ultrasound-assisted extraction of polysaccharide-protein complexes from β-glucan-rich medicinal mushrooms for enhanced antioxidant activity |
| title_sort | modulating ultrasound assisted extraction of polysaccharide protein complexes from β glucan rich medicinal mushrooms for enhanced antioxidant activity |
| topic | Ultrasound-assisted extraction Polysaccharide-protein complexes Medicinal mushroom Antioxidant activity Response surface methodology β-glucan |
| url | http://www.sciencedirect.com/science/article/pii/S2666893925001550 |
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