Co-Circulation of Coronaviruses among Rodents and Insectivores
Coronaviruses (family Coronaviridae, genera Alphacoronavirus and Betacoronavirus) are the causative agents of respiratory, intestinal and neurological diseases in humans and animals. Natural reservoirs of coronaviruses include bats, rodents and insectivores, however, the circulation of coronaviruses...
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2023-07-01
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| Series: | Проблемы особо опасных инфекций |
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| Online Access: | https://journal.microbe.ru/jour/article/view/1836 |
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| author | L. N. Yashina N. A. Smetannikova V. V. Panov |
| author_facet | L. N. Yashina N. A. Smetannikova V. V. Panov |
| author_sort | L. N. Yashina |
| collection | DOAJ |
| description | Coronaviruses (family Coronaviridae, genera Alphacoronavirus and Betacoronavirus) are the causative agents of respiratory, intestinal and neurological diseases in humans and animals. Natural reservoirs of coronaviruses include bats, rodents and insectivores, however, the circulation of coronaviruses among rodents and insectivores in the Russian Federation has been unexplored. The aim of the study was to investigate the diversity of coronaviruses among rodents and insectivores co-inhabiting natural biotopes. Materials and methods. Rodents (68 specimens) and shrews of the genus Sorex (23 specimens) were caught in a limited forest area not exceeding 1.5 sq. km, in the vicinity of Novosibirsk. All samples were screened using reverse transcription polymerase chain reaction followed by sequencing. Results and discussion. Four distinct coronaviruses have been detected in four species of small mammals. Rodent-borne coronaviruses were classed within subgenera Embecovirus, genus Betacoronavirus, and demonstrated host-associated phylogenetic clustering. The level of homology between the new RNA isolates from red-backed vole (Myodes rutilus), root vole (Microtus oeconomus) and field mouse (Apodemus agrarius) is 85.5–87.7 %. The nucleotide sequences of Siberian coronavirus isolates are closely related (>93 % homology) to previously published sequences in each of the carrier groups found in Europe and China, which suggests their common evolutionary origin. The coronavirus identified in the common shrew (Sorex araneus) belongs to the genus Alphacoronavirus, but is significantly different (>36 % difference) from earlier identified strains included in the genus. It has been shown that different coronaviruses co-circulate in a limited area among rodents and insectivores. |
| format | Article |
| id | doaj-art-7550c887922341e69987a3ee6c8db4f6 |
| institution | Kabale University |
| issn | 0370-1069 2658-719X |
| language | Russian |
| publishDate | 2023-07-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj-art-7550c887922341e69987a3ee6c8db4f62025-08-20T03:50:11ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2023-07-010216717210.21055/0370-1069-2023-2-167-1721471Co-Circulation of Coronaviruses among Rodents and InsectivoresL. N. Yashina0N. A. Smetannikova1V. V. Panov2State Research Center of Virology and Biotechnology “Vector”State Research Center of Virology and Biotechnology “Vector”Institute of Systematics and Ecology of Animals, Siberian Branch of RASCoronaviruses (family Coronaviridae, genera Alphacoronavirus and Betacoronavirus) are the causative agents of respiratory, intestinal and neurological diseases in humans and animals. Natural reservoirs of coronaviruses include bats, rodents and insectivores, however, the circulation of coronaviruses among rodents and insectivores in the Russian Federation has been unexplored. The aim of the study was to investigate the diversity of coronaviruses among rodents and insectivores co-inhabiting natural biotopes. Materials and methods. Rodents (68 specimens) and shrews of the genus Sorex (23 specimens) were caught in a limited forest area not exceeding 1.5 sq. km, in the vicinity of Novosibirsk. All samples were screened using reverse transcription polymerase chain reaction followed by sequencing. Results and discussion. Four distinct coronaviruses have been detected in four species of small mammals. Rodent-borne coronaviruses were classed within subgenera Embecovirus, genus Betacoronavirus, and demonstrated host-associated phylogenetic clustering. The level of homology between the new RNA isolates from red-backed vole (Myodes rutilus), root vole (Microtus oeconomus) and field mouse (Apodemus agrarius) is 85.5–87.7 %. The nucleotide sequences of Siberian coronavirus isolates are closely related (>93 % homology) to previously published sequences in each of the carrier groups found in Europe and China, which suggests their common evolutionary origin. The coronavirus identified in the common shrew (Sorex araneus) belongs to the genus Alphacoronavirus, but is significantly different (>36 % difference) from earlier identified strains included in the genus. It has been shown that different coronaviruses co-circulate in a limited area among rodents and insectivores.https://journal.microbe.ru/jour/article/view/1836coronavirusrodentsinsectivoressiberia |
| spellingShingle | L. N. Yashina N. A. Smetannikova V. V. Panov Co-Circulation of Coronaviruses among Rodents and Insectivores Проблемы особо опасных инфекций coronavirus rodents insectivores siberia |
| title | Co-Circulation of Coronaviruses among Rodents and Insectivores |
| title_full | Co-Circulation of Coronaviruses among Rodents and Insectivores |
| title_fullStr | Co-Circulation of Coronaviruses among Rodents and Insectivores |
| title_full_unstemmed | Co-Circulation of Coronaviruses among Rodents and Insectivores |
| title_short | Co-Circulation of Coronaviruses among Rodents and Insectivores |
| title_sort | co circulation of coronaviruses among rodents and insectivores |
| topic | coronavirus rodents insectivores siberia |
| url | https://journal.microbe.ru/jour/article/view/1836 |
| work_keys_str_mv | AT lnyashina cocirculationofcoronavirusesamongrodentsandinsectivores AT nasmetannikova cocirculationofcoronavirusesamongrodentsandinsectivores AT vvpanov cocirculationofcoronavirusesamongrodentsandinsectivores |