Development and validation of polymer-based porphyrin-incorporated reference materials for calibration of quantitative light-induced fluorescence device
Background: This study aimed at developing and validating polymer-based reference materials with varying amounts of porphyrin to accurately assess and calibrate quantitative light-induced fluorescence (QLF) device. Methods: Reference materials with porphyrin concentrations ranging from 0 to 0.08 wt....
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Elsevier
2024-12-01
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| Series: | Photodiagnosis and Photodynamic Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1572100024004289 |
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| author | Sang-Eun Park Hong-Cheol Yoon Elbert de Josselin de Jong Kwang-Mahn Kim Jae-Sung Kwon |
| author_facet | Sang-Eun Park Hong-Cheol Yoon Elbert de Josselin de Jong Kwang-Mahn Kim Jae-Sung Kwon |
| author_sort | Sang-Eun Park |
| collection | DOAJ |
| description | Background: This study aimed at developing and validating polymer-based reference materials with varying amounts of porphyrin to accurately assess and calibrate quantitative light-induced fluorescence (QLF) device. Methods: Reference materials with porphyrin concentrations ranging from 0 to 0.08 wt.% were prepared. The surface properties of the materials were analyzed via gloss and roughness measurements. Color analysis of the specimens was performed on black and white backgrounds with or without filters using a spectrophotometer. This approach revealed the correlations between fluorescence and color. The fluorescence emitted by the specimens was analyzed by measuring ΔR and ΔRmax values using a QLF-D Biluminator™. Results: The surface gloss and roughness of the reference materials were not affected by the porphyrin content (p > 0.005). Spectrophotometric measurements revealed significant color differences among most specimen groups depending on the background color and the presence of a filter. QLF-D imaging revealed significant differences in fluorescence (ΔR and ΔRmax) among all specimen groups regardless of the background. The fluorescence values observed on the black backgrounds were higher than those observed on the white backgrounds (p < 0.05). Conclusions: The developed polymer-based porphyrin-incorporated materials serve as reliable reference standards for accurate assessment and calibration of QLF devices. This study demonstrates the importance of background conditions in fluorescence detection and highlights the potential of these materials as standards for QLF device calibration. |
| format | Article |
| id | doaj-art-754d84a9cfa347409e65ca2d5f5fb400 |
| institution | OA Journals |
| issn | 1572-1000 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Photodiagnosis and Photodynamic Therapy |
| spelling | doaj-art-754d84a9cfa347409e65ca2d5f5fb4002025-08-20T01:59:30ZengElsevierPhotodiagnosis and Photodynamic Therapy1572-10002024-12-015010439110.1016/j.pdpdt.2024.104391Development and validation of polymer-based porphyrin-incorporated reference materials for calibration of quantitative light-induced fluorescence deviceSang-Eun Park0Hong-Cheol Yoon1Elbert de Josselin de Jong2Kwang-Mahn Kim3Jae-Sung Kwon4Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul, Republic of Korea; BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, Republic of KoreaBestden Dental Clinic, Seoul, Republic of KoreaBK21 FOUR Project, Yonsei University College of Dentistry, Seoul, Republic of Korea; Inspektor Research Systems BV, Amsterdam, the NetherlandsDepartment and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul, Republic of Korea; Corresponding authors at: Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul, Republic of Korea; BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, Republic of Korea; Corresponding authors at: Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.Background: This study aimed at developing and validating polymer-based reference materials with varying amounts of porphyrin to accurately assess and calibrate quantitative light-induced fluorescence (QLF) device. Methods: Reference materials with porphyrin concentrations ranging from 0 to 0.08 wt.% were prepared. The surface properties of the materials were analyzed via gloss and roughness measurements. Color analysis of the specimens was performed on black and white backgrounds with or without filters using a spectrophotometer. This approach revealed the correlations between fluorescence and color. The fluorescence emitted by the specimens was analyzed by measuring ΔR and ΔRmax values using a QLF-D Biluminator™. Results: The surface gloss and roughness of the reference materials were not affected by the porphyrin content (p > 0.005). Spectrophotometric measurements revealed significant color differences among most specimen groups depending on the background color and the presence of a filter. QLF-D imaging revealed significant differences in fluorescence (ΔR and ΔRmax) among all specimen groups regardless of the background. The fluorescence values observed on the black backgrounds were higher than those observed on the white backgrounds (p < 0.05). Conclusions: The developed polymer-based porphyrin-incorporated materials serve as reliable reference standards for accurate assessment and calibration of QLF devices. This study demonstrates the importance of background conditions in fluorescence detection and highlights the potential of these materials as standards for QLF device calibration.http://www.sciencedirect.com/science/article/pii/S1572100024004289Quantitative light-induced fluorescencePorphyrinReference materialRed fluorescenceCalibration standard |
| spellingShingle | Sang-Eun Park Hong-Cheol Yoon Elbert de Josselin de Jong Kwang-Mahn Kim Jae-Sung Kwon Development and validation of polymer-based porphyrin-incorporated reference materials for calibration of quantitative light-induced fluorescence device Photodiagnosis and Photodynamic Therapy Quantitative light-induced fluorescence Porphyrin Reference material Red fluorescence Calibration standard |
| title | Development and validation of polymer-based porphyrin-incorporated reference materials for calibration of quantitative light-induced fluorescence device |
| title_full | Development and validation of polymer-based porphyrin-incorporated reference materials for calibration of quantitative light-induced fluorescence device |
| title_fullStr | Development and validation of polymer-based porphyrin-incorporated reference materials for calibration of quantitative light-induced fluorescence device |
| title_full_unstemmed | Development and validation of polymer-based porphyrin-incorporated reference materials for calibration of quantitative light-induced fluorescence device |
| title_short | Development and validation of polymer-based porphyrin-incorporated reference materials for calibration of quantitative light-induced fluorescence device |
| title_sort | development and validation of polymer based porphyrin incorporated reference materials for calibration of quantitative light induced fluorescence device |
| topic | Quantitative light-induced fluorescence Porphyrin Reference material Red fluorescence Calibration standard |
| url | http://www.sciencedirect.com/science/article/pii/S1572100024004289 |
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