Manganese‐enhanced MRI during remotely induced myocardial ischemia reperfusion injury in male mice
Abstract Early assessment of myocardial viability post‐ischemia is crucial to mitigate adverse remodeling and optimize therapy. Current noninvasive methods like late gadolinium enhancement (LGE) MRI may overestimate infarct size. Manganese‐enhanced MRI (MEMRI) emerged as a promising alternative, off...
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Wiley
2025-07-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.70442 |
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| author | Matic Pusovnik Michiel Algoet Willy Gsell Stefan Janssens Wouter Oosterlinck Uwe Himmelreich |
| author_facet | Matic Pusovnik Michiel Algoet Willy Gsell Stefan Janssens Wouter Oosterlinck Uwe Himmelreich |
| author_sort | Matic Pusovnik |
| collection | DOAJ |
| description | Abstract Early assessment of myocardial viability post‐ischemia is crucial to mitigate adverse remodeling and optimize therapy. Current noninvasive methods like late gadolinium enhancement (LGE) MRI may overestimate infarct size. Manganese‐enhanced MRI (MEMRI) emerged as a promising alternative, offering greater specificity in assessing myocardial damage. We evaluated MEMRI alongside LGE and histology in a murine ischemia–reperfusion model using a novel “in‐scanner” remote occlusion technique for real‐time imaging during acute ischemia. Male C57BL/6 mice (n = 16) underwent left anterior descending artery occlusion (n = 7), sham surgery (n = 6), or no intervention (n = 3). MEMRI (0.1 mmol/kg MnCl2) during ischemia (0–60 min) and LGE (0.1 mmol/kg Gd‐DOTA, 24 h post‐surgery) quantified perfusion deficits and infarct size. MEMRI detected acute hypo‐perfusion (lateral wall signal reduction: p < 0.01 vs. septal), confined to the occluded territory, while LGE overestimated infarct size (p = 0.0225 vs. histology). Ischemic mice showed adverse remodeling with reduced ejection fraction (61.37% vs. 71.92%, p < 0.01). MEMRI‐derived perfusion deficits correlated with functional decline and histology‐confirmed infarcts. Pre‐occlusion T1 times did not differ between ischemic and sham groups (p = 0.85), confirming technique specificity. MEMRI enables early, accurate ischemic injury detection and predicts cardiac dysfunction, outperforming LGE in infarct size determination. Our remote occlusion technique facilitates real‐time perfusion assessments, enhancing preclinical myocardial ischemia studies. |
| format | Article |
| id | doaj-art-754cd599fb1d4098a91ba0176811c2d4 |
| institution | Kabale University |
| issn | 2051-817X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj-art-754cd599fb1d4098a91ba0176811c2d42025-08-20T03:50:49ZengWileyPhysiological Reports2051-817X2025-07-011313n/an/a10.14814/phy2.70442Manganese‐enhanced MRI during remotely induced myocardial ischemia reperfusion injury in male miceMatic Pusovnik0Michiel Algoet1Willy Gsell2Stefan Janssens3Wouter Oosterlinck4Uwe Himmelreich5Biomedical MRI, Department of Imaging and Pathology KU Leuven Leuven BelgiumDepartment of Cardiovascular Sciences KU Leuven Leuven BelgiumBruker Belgium Kontich BelgiumDepartment of Cardiovascular Sciences KU Leuven Leuven BelgiumDepartment of Cardiovascular Sciences KU Leuven Leuven BelgiumBiomedical MRI, Department of Imaging and Pathology KU Leuven Leuven BelgiumAbstract Early assessment of myocardial viability post‐ischemia is crucial to mitigate adverse remodeling and optimize therapy. Current noninvasive methods like late gadolinium enhancement (LGE) MRI may overestimate infarct size. Manganese‐enhanced MRI (MEMRI) emerged as a promising alternative, offering greater specificity in assessing myocardial damage. We evaluated MEMRI alongside LGE and histology in a murine ischemia–reperfusion model using a novel “in‐scanner” remote occlusion technique for real‐time imaging during acute ischemia. Male C57BL/6 mice (n = 16) underwent left anterior descending artery occlusion (n = 7), sham surgery (n = 6), or no intervention (n = 3). MEMRI (0.1 mmol/kg MnCl2) during ischemia (0–60 min) and LGE (0.1 mmol/kg Gd‐DOTA, 24 h post‐surgery) quantified perfusion deficits and infarct size. MEMRI detected acute hypo‐perfusion (lateral wall signal reduction: p < 0.01 vs. septal), confined to the occluded territory, while LGE overestimated infarct size (p = 0.0225 vs. histology). Ischemic mice showed adverse remodeling with reduced ejection fraction (61.37% vs. 71.92%, p < 0.01). MEMRI‐derived perfusion deficits correlated with functional decline and histology‐confirmed infarcts. Pre‐occlusion T1 times did not differ between ischemic and sham groups (p = 0.85), confirming technique specificity. MEMRI enables early, accurate ischemic injury detection and predicts cardiac dysfunction, outperforming LGE in infarct size determination. Our remote occlusion technique facilitates real‐time perfusion assessments, enhancing preclinical myocardial ischemia studies.https://doi.org/10.14814/phy2.70442ischemia–reperfusion injuryleft anterior descending arterymanganese enhanced magnetic resonance imagingmyocardial perfusionmyocardial remodeling |
| spellingShingle | Matic Pusovnik Michiel Algoet Willy Gsell Stefan Janssens Wouter Oosterlinck Uwe Himmelreich Manganese‐enhanced MRI during remotely induced myocardial ischemia reperfusion injury in male mice Physiological Reports ischemia–reperfusion injury left anterior descending artery manganese enhanced magnetic resonance imaging myocardial perfusion myocardial remodeling |
| title | Manganese‐enhanced MRI during remotely induced myocardial ischemia reperfusion injury in male mice |
| title_full | Manganese‐enhanced MRI during remotely induced myocardial ischemia reperfusion injury in male mice |
| title_fullStr | Manganese‐enhanced MRI during remotely induced myocardial ischemia reperfusion injury in male mice |
| title_full_unstemmed | Manganese‐enhanced MRI during remotely induced myocardial ischemia reperfusion injury in male mice |
| title_short | Manganese‐enhanced MRI during remotely induced myocardial ischemia reperfusion injury in male mice |
| title_sort | manganese enhanced mri during remotely induced myocardial ischemia reperfusion injury in male mice |
| topic | ischemia–reperfusion injury left anterior descending artery manganese enhanced magnetic resonance imaging myocardial perfusion myocardial remodeling |
| url | https://doi.org/10.14814/phy2.70442 |
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