Protective Effects of Olanzapine on Irradiation-Induced Pulmonary and Cardiac Injury in Mice: A Histopathological Evaluation
Background and purpose: Radiation therapy (IR) is a standard treatment for various thoracic malignancies. However, radiation-induced pulmonary and cardiac injuries can be life-threatening complications in these patients. Olanzapine (OLZ) is an atypical antipsychotic drug that exhibits antioxidant pr...
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Mazandaran University of Medical Sciences
2025-08-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
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| Online Access: | http://jmums.mazums.ac.ir/article-1-21985-en.pdf |
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| author | Ali Mirmahdi Mansoureh Mirzaei Seyed Jalal Hosseinimehr Abbas Ali Karimpour Malakshah Hooman Zarei Fereshteh Talebpour Amiri |
| author_facet | Ali Mirmahdi Mansoureh Mirzaei Seyed Jalal Hosseinimehr Abbas Ali Karimpour Malakshah Hooman Zarei Fereshteh Talebpour Amiri |
| author_sort | Ali Mirmahdi |
| collection | DOAJ |
| description | Background and purpose: Radiation therapy (IR) is a standard treatment for various thoracic malignancies. However, radiation-induced pulmonary and cardiac injuries can be life-threatening complications in these patients. Olanzapine (OLZ) is an atypical antipsychotic drug that exhibits antioxidant properties at low doses. The aim of this study was to evaluate the histopathological changes associated with radiotherapy-induced pneumonitis and cardiac injury, and to assess the protective effects of olanzapine against these conditions.
Materials and methods: In this experimental study, 30 BALB/c mice were randomly assigned to six groups: Control, IR (6 Gy, single dose on the eighth day of the study), OLZ (10 and 20 mg/kg for 10 consecutive days), IR+ OLZ (10 mg/kg), and IR + OLZ (20 mg/kg). Histopathological analysis was performed to assess structural changes in the myocardium and lungs on day 11 of the study. Data were analyzed using GraphPad Prism software, and statistical comparisons were made using one-way ANOVA followed by Tukey's post hoc test.
Results: Our data showed that radiotherapy induced cardiac and pulmonary injury. Histopathological analysis revealed that olanzapine ameliorated structural cardiac damage, including fibrosis and macrophage infiltration, in irradiated mice. In lung histology, olanzapine reduced interstitial edema, inflammatory cell infiltration, alveolar wall thickening, alveolar collapse, and vascular congestion. Semi-quantitative evaluation demonstrated that the 20 mg/kg dose was more effective than the 10 mg/kg dose and the radiation-only group in improving the tissue structure of both the heart and lungs.
Conclusion: Overall, our study demonstrated the protective effects of olanzapine treatment against pulmonary and cardiac injuries, as evidenced by improvements in histological structure. |
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| institution | Kabale University |
| issn | 1735-9260 1735-9279 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Mazandaran University of Medical Sciences |
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| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj-art-7543576bc2dc4438a05f1663f580f1672025-08-20T05:26:28ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792025-08-0135247312Protective Effects of Olanzapine on Irradiation-Induced Pulmonary and Cardiac Injury in Mice: A Histopathological EvaluationAli Mirmahdi0Mansoureh Mirzaei1Seyed Jalal Hosseinimehr2Abbas Ali Karimpour Malakshah3Hooman Zarei4Fereshteh Talebpour Amiri5 PhD in Comparative Histology, Mazandaran University of Medical Sciences, Sari, Iran Professor, Department of Nuclear Pharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Professor, Department of Anatomical Sciences, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Science, Sari, Iran Assistant Professor, Department of Anatomical Sciences, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Science, Sari, Iran Professor, Department of Anatomical Sciences, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Science, Sari, Iran Background and purpose: Radiation therapy (IR) is a standard treatment for various thoracic malignancies. However, radiation-induced pulmonary and cardiac injuries can be life-threatening complications in these patients. Olanzapine (OLZ) is an atypical antipsychotic drug that exhibits antioxidant properties at low doses. The aim of this study was to evaluate the histopathological changes associated with radiotherapy-induced pneumonitis and cardiac injury, and to assess the protective effects of olanzapine against these conditions. Materials and methods: In this experimental study, 30 BALB/c mice were randomly assigned to six groups: Control, IR (6 Gy, single dose on the eighth day of the study), OLZ (10 and 20 mg/kg for 10 consecutive days), IR+ OLZ (10 mg/kg), and IR + OLZ (20 mg/kg). Histopathological analysis was performed to assess structural changes in the myocardium and lungs on day 11 of the study. Data were analyzed using GraphPad Prism software, and statistical comparisons were made using one-way ANOVA followed by Tukey's post hoc test. Results: Our data showed that radiotherapy induced cardiac and pulmonary injury. Histopathological analysis revealed that olanzapine ameliorated structural cardiac damage, including fibrosis and macrophage infiltration, in irradiated mice. In lung histology, olanzapine reduced interstitial edema, inflammatory cell infiltration, alveolar wall thickening, alveolar collapse, and vascular congestion. Semi-quantitative evaluation demonstrated that the 20 mg/kg dose was more effective than the 10 mg/kg dose and the radiation-only group in improving the tissue structure of both the heart and lungs. Conclusion: Overall, our study demonstrated the protective effects of olanzapine treatment against pulmonary and cardiac injuries, as evidenced by improvements in histological structure.http://jmums.mazums.ac.ir/article-1-21985-en.pdfirradiationcardiotoxicitylung injuryhistopathology |
| spellingShingle | Ali Mirmahdi Mansoureh Mirzaei Seyed Jalal Hosseinimehr Abbas Ali Karimpour Malakshah Hooman Zarei Fereshteh Talebpour Amiri Protective Effects of Olanzapine on Irradiation-Induced Pulmonary and Cardiac Injury in Mice: A Histopathological Evaluation Journal of Mazandaran University of Medical Sciences irradiation cardiotoxicity lung injury histopathology |
| title | Protective Effects of Olanzapine on Irradiation-Induced Pulmonary and Cardiac Injury in Mice: A Histopathological Evaluation |
| title_full | Protective Effects of Olanzapine on Irradiation-Induced Pulmonary and Cardiac Injury in Mice: A Histopathological Evaluation |
| title_fullStr | Protective Effects of Olanzapine on Irradiation-Induced Pulmonary and Cardiac Injury in Mice: A Histopathological Evaluation |
| title_full_unstemmed | Protective Effects of Olanzapine on Irradiation-Induced Pulmonary and Cardiac Injury in Mice: A Histopathological Evaluation |
| title_short | Protective Effects of Olanzapine on Irradiation-Induced Pulmonary and Cardiac Injury in Mice: A Histopathological Evaluation |
| title_sort | protective effects of olanzapine on irradiation induced pulmonary and cardiac injury in mice a histopathological evaluation |
| topic | irradiation cardiotoxicity lung injury histopathology |
| url | http://jmums.mazums.ac.ir/article-1-21985-en.pdf |
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