Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis.
Rapidly-frozen hydrated (cryopreserved) specimens combined with cryo-scanning x-ray fluorescence microscopy provide an ideal approach for investigating elemental distributions in biological cells and tissues. However, because cryopreservation does not deactivate potentially infectious agents associa...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117437&type=printable |
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| author | Qiaoling Jin Stefan Vogt Barry Lai Si Chen Lydia Finney Sophie-Charlotte Gleber Jesse Ward Junjing Deng Rachel Mak Nena Moonier Chris Jacobsen |
| author_facet | Qiaoling Jin Stefan Vogt Barry Lai Si Chen Lydia Finney Sophie-Charlotte Gleber Jesse Ward Junjing Deng Rachel Mak Nena Moonier Chris Jacobsen |
| author_sort | Qiaoling Jin |
| collection | DOAJ |
| description | Rapidly-frozen hydrated (cryopreserved) specimens combined with cryo-scanning x-ray fluorescence microscopy provide an ideal approach for investigating elemental distributions in biological cells and tissues. However, because cryopreservation does not deactivate potentially infectious agents associated with Risk Group 2 biological materials, one must be concerned with contamination of expensive and complicated cryogenic x-ray microscopes when working with such materials. We employed ultraviolet germicidal irradiation to decontaminate previously cryopreserved cells under liquid nitrogen, and then investigated its effects on elemental distributions under both frozen hydrated and freeze dried states with x-ray fluorescence microscopy. We show that the contents and distributions of most biologically important elements remain nearly unchanged when compared with non-ultraviolet-irradiated counterparts, even after multiple cycles of ultraviolet germicidal irradiation and cryogenic x-ray imaging. This provides a potential pathway for rendering Risk Group 2 biological materials safe for handling in multiuser cryogenic x-ray microscopes without affecting the fidelity of the results. |
| format | Article |
| id | doaj-art-752a47b094c8410fad0d358acf2affc8 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-752a47b094c8410fad0d358acf2affc82025-08-20T03:01:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011743710.1371/journal.pone.0117437Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis.Qiaoling JinStefan VogtBarry LaiSi ChenLydia FinneySophie-Charlotte GleberJesse WardJunjing DengRachel MakNena MoonierChris JacobsenRapidly-frozen hydrated (cryopreserved) specimens combined with cryo-scanning x-ray fluorescence microscopy provide an ideal approach for investigating elemental distributions in biological cells and tissues. However, because cryopreservation does not deactivate potentially infectious agents associated with Risk Group 2 biological materials, one must be concerned with contamination of expensive and complicated cryogenic x-ray microscopes when working with such materials. We employed ultraviolet germicidal irradiation to decontaminate previously cryopreserved cells under liquid nitrogen, and then investigated its effects on elemental distributions under both frozen hydrated and freeze dried states with x-ray fluorescence microscopy. We show that the contents and distributions of most biologically important elements remain nearly unchanged when compared with non-ultraviolet-irradiated counterparts, even after multiple cycles of ultraviolet germicidal irradiation and cryogenic x-ray imaging. This provides a potential pathway for rendering Risk Group 2 biological materials safe for handling in multiuser cryogenic x-ray microscopes without affecting the fidelity of the results.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117437&type=printable |
| spellingShingle | Qiaoling Jin Stefan Vogt Barry Lai Si Chen Lydia Finney Sophie-Charlotte Gleber Jesse Ward Junjing Deng Rachel Mak Nena Moonier Chris Jacobsen Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis. PLoS ONE |
| title | Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis. |
| title_full | Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis. |
| title_fullStr | Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis. |
| title_full_unstemmed | Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis. |
| title_short | Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis. |
| title_sort | ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x ray fluorescence microanalysis |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117437&type=printable |
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