Viral load of human papillomavirus in women with normal and abnormal cervical cytology in Kuwait
Introduction: Human papillomaviruses (HPV) are the most commonly known sexually transmitted agents. Almost all cases of cervical cancer are caused by persistent infection. This study was conducted to ascertain whether there is a difference in HPV load in cervical samples with normal and abnormal ce...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2013-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/2748 |
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| author | Rana Al-Awadhi Wassim Chehadeh Waleed Al-Jassar Jehad Al-Harmi Eyad Al-Saleh Kusum Kapila |
| author_facet | Rana Al-Awadhi Wassim Chehadeh Waleed Al-Jassar Jehad Al-Harmi Eyad Al-Saleh Kusum Kapila |
| author_sort | Rana Al-Awadhi |
| collection | DOAJ |
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Introduction: Human papillomaviruses (HPV) are the most commonly known sexually transmitted agents. Almost all cases of cervical cancer are caused by persistent infection. This study was conducted to ascertain whether there is a difference in HPV load in cervical samples with normal and abnormal cervical cytology reports in Kuwait.
Methodology: HPV-positive abnormal ThinPrep samples (n = 206) and normal ThinPrep samples (n = 120) were taken from women attending gynecology clinics. Real-time PCR was used to measure the viral load for all HPV genotypes.
Results: The median normalized viral load in samples with normal and abnormal cytology reports was 0.86 × 10-7 and 4.66 × 10-7, respectively (p = 0.001). Median normalized viral load of high-risk (HR), intermediate-risk (IR) and low-risk (LR) HPV was 4.04 × 10-7, 0.71 × 10-7 and 2.38 × 10-7, respectively, (p = 0.002).
Conclusions: The findings suggest that, in the absence of a proper screening programme in Kuwait, quantification of HPV viral load could be considered as a surrogate virology test to identify women with abnormal cytology. Further population-based prospective studies are needed to include more women with high-grade and invasive carcinoma reports.
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| format | Article |
| id | doaj-art-751ee3e6db2b44f4ad751d9ae58ca4f7 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2013-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-751ee3e6db2b44f4ad751d9ae58ca4f72025-08-20T03:48:57ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802013-02-0170210.3855/jidc.2748Viral load of human papillomavirus in women with normal and abnormal cervical cytology in KuwaitRana Al-Awadhi0Wassim Chehadeh1Waleed Al-Jassar2Jehad Al-Harmi3Eyad Al-Saleh4Kusum Kapila5Faculty of Allied Health Sciences, Kuwait University, KuwaitFaculty of Medicine, Kuwait University, KuwaitFaculty of Medicine, Kuwait University, KuwaitFaculty of Medicine, Kuwait University, KuwaitFaculty of Medicine, Kuwait University, KuwaitFaculty of Medicine, Kuwait University, Kuwait Introduction: Human papillomaviruses (HPV) are the most commonly known sexually transmitted agents. Almost all cases of cervical cancer are caused by persistent infection. This study was conducted to ascertain whether there is a difference in HPV load in cervical samples with normal and abnormal cervical cytology reports in Kuwait. Methodology: HPV-positive abnormal ThinPrep samples (n = 206) and normal ThinPrep samples (n = 120) were taken from women attending gynecology clinics. Real-time PCR was used to measure the viral load for all HPV genotypes. Results: The median normalized viral load in samples with normal and abnormal cytology reports was 0.86 × 10-7 and 4.66 × 10-7, respectively (p = 0.001). Median normalized viral load of high-risk (HR), intermediate-risk (IR) and low-risk (LR) HPV was 4.04 × 10-7, 0.71 × 10-7 and 2.38 × 10-7, respectively, (p = 0.002). Conclusions: The findings suggest that, in the absence of a proper screening programme in Kuwait, quantification of HPV viral load could be considered as a surrogate virology test to identify women with abnormal cytology. Further population-based prospective studies are needed to include more women with high-grade and invasive carcinoma reports. https://jidc.org/index.php/journal/article/view/2748HPVreal-time PCR quantitative assayviral loadwomen with normal and abnormal cervical cytology |
| spellingShingle | Rana Al-Awadhi Wassim Chehadeh Waleed Al-Jassar Jehad Al-Harmi Eyad Al-Saleh Kusum Kapila Viral load of human papillomavirus in women with normal and abnormal cervical cytology in Kuwait Journal of Infection in Developing Countries HPV real-time PCR quantitative assay viral load women with normal and abnormal cervical cytology |
| title | Viral load of human papillomavirus in women with normal and abnormal cervical cytology in Kuwait |
| title_full | Viral load of human papillomavirus in women with normal and abnormal cervical cytology in Kuwait |
| title_fullStr | Viral load of human papillomavirus in women with normal and abnormal cervical cytology in Kuwait |
| title_full_unstemmed | Viral load of human papillomavirus in women with normal and abnormal cervical cytology in Kuwait |
| title_short | Viral load of human papillomavirus in women with normal and abnormal cervical cytology in Kuwait |
| title_sort | viral load of human papillomavirus in women with normal and abnormal cervical cytology in kuwait |
| topic | HPV real-time PCR quantitative assay viral load women with normal and abnormal cervical cytology |
| url | https://jidc.org/index.php/journal/article/view/2748 |
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