Function analysis of LIPI-4 in Listeria monocytogenes reveals a key role of flagella formation in the regulation of virulence
Listeria pathogenicity island 4 (LIPI-4), a newly discovered virulence gene cluster, plays a critical role in the development of central nervous system infections and maternal-fetal infections caused by Listeria monocytogenes. This study aimed to clarify the pathogenic role of LIPI-4 in L. monocytog...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Virulence |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2025.2543144 |
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| author | Caixia Liu Xun Ma Bin Jia Ruixuan Qian Shengjie Gao Lu Liu Yatao Qi Zhongke Yin Guangdong Hu Jing Wang |
| author_facet | Caixia Liu Xun Ma Bin Jia Ruixuan Qian Shengjie Gao Lu Liu Yatao Qi Zhongke Yin Guangdong Hu Jing Wang |
| author_sort | Caixia Liu |
| collection | DOAJ |
| description | Listeria pathogenicity island 4 (LIPI-4), a newly discovered virulence gene cluster, plays a critical role in the development of central nervous system infections and maternal-fetal infections caused by Listeria monocytogenes. This study aimed to clarify the pathogenic role of LIPI-4 in L. monocytogenes. Our findings revealed that LIPI-4 is involved in the invasion and intracellular proliferation of L. monocytogenes in Caco-2, hCMEC/D3, HTR-8/Svneo, and RAW264.7 cells and facilitates its intercellular migration. Further investigations demonstrated that the deletion of LIPI-4 significantly reduces bacterial motility and impairs flagellum formation. Transcriptomic analysis further indicated that LIPI-4 regulates flagellum formation by modulating microbial metabolic processes and carbon metabolism. Our research demonstrates that LIPI-4 promotes flagellum formation through metabolic regulation, thereby enhancing L. monocytogenes colonization and cellular invasion. This discovery provides novel insights into the pathogenic mechanisms of L. monocytogenes. |
| format | Article |
| id | doaj-art-75153f6dc1e04cb4bf7ed85f7321af3b |
| institution | Kabale University |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-75153f6dc1e04cb4bf7ed85f7321af3b2025-08-20T03:59:25ZengTaylor & Francis GroupVirulence2150-55942150-56082025-12-0116110.1080/21505594.2025.2543144Function analysis of LIPI-4 in Listeria monocytogenes reveals a key role of flagella formation in the regulation of virulenceCaixia Liu0Xun Ma1Bin Jia2Ruixuan Qian3Shengjie Gao4Lu Liu5Yatao Qi6Zhongke Yin7Guangdong Hu8Jing Wang9College of Animal Science and Technology, Shihezi University, Shihezi, PR ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi, PR ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi, PR ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi, PR ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi, PR ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi, PR ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi, PR ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi, PR ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi, PR ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi, PR ChinaListeria pathogenicity island 4 (LIPI-4), a newly discovered virulence gene cluster, plays a critical role in the development of central nervous system infections and maternal-fetal infections caused by Listeria monocytogenes. This study aimed to clarify the pathogenic role of LIPI-4 in L. monocytogenes. Our findings revealed that LIPI-4 is involved in the invasion and intracellular proliferation of L. monocytogenes in Caco-2, hCMEC/D3, HTR-8/Svneo, and RAW264.7 cells and facilitates its intercellular migration. Further investigations demonstrated that the deletion of LIPI-4 significantly reduces bacterial motility and impairs flagellum formation. Transcriptomic analysis further indicated that LIPI-4 regulates flagellum formation by modulating microbial metabolic processes and carbon metabolism. Our research demonstrates that LIPI-4 promotes flagellum formation through metabolic regulation, thereby enhancing L. monocytogenes colonization and cellular invasion. This discovery provides novel insights into the pathogenic mechanisms of L. monocytogenes.https://www.tandfonline.com/doi/10.1080/21505594.2025.2543144Listeria monocytogenesLIPI-4flagellatranscriptomicsVirulence |
| spellingShingle | Caixia Liu Xun Ma Bin Jia Ruixuan Qian Shengjie Gao Lu Liu Yatao Qi Zhongke Yin Guangdong Hu Jing Wang Function analysis of LIPI-4 in Listeria monocytogenes reveals a key role of flagella formation in the regulation of virulence Virulence Listeria monocytogenes LIPI-4 flagella transcriptomics Virulence |
| title | Function analysis of LIPI-4 in Listeria monocytogenes reveals a key role of flagella formation in the regulation of virulence |
| title_full | Function analysis of LIPI-4 in Listeria monocytogenes reveals a key role of flagella formation in the regulation of virulence |
| title_fullStr | Function analysis of LIPI-4 in Listeria monocytogenes reveals a key role of flagella formation in the regulation of virulence |
| title_full_unstemmed | Function analysis of LIPI-4 in Listeria monocytogenes reveals a key role of flagella formation in the regulation of virulence |
| title_short | Function analysis of LIPI-4 in Listeria monocytogenes reveals a key role of flagella formation in the regulation of virulence |
| title_sort | function analysis of lipi 4 in listeria monocytogenes reveals a key role of flagella formation in the regulation of virulence |
| topic | Listeria monocytogenes LIPI-4 flagella transcriptomics Virulence |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2025.2543144 |
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