Mycosis fungoides progression could be regulated by microRNAs.
Differentiating early mycosis fungoides (MF) from inflammatory dermatitis is a challenge. We compare the differential expression profile of early-stage MF samples and benign inflammatory dermatoses using microRNA (miRNA) arrays. 114 miRNAs were found to be dysregulated between these entities. The se...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0198477 |
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| author | Rebeca Manso Nerea Martínez-Magunacelaya Itziar Eraña-Tomás Verónica Monsalvez José L Rodríguez-Peralto Pablo-L Ortiz-Romero Carlos Santonja Ion Cristóbal Miguel A Piris Socorro M Rodríguez-Pinilla |
| author_facet | Rebeca Manso Nerea Martínez-Magunacelaya Itziar Eraña-Tomás Verónica Monsalvez José L Rodríguez-Peralto Pablo-L Ortiz-Romero Carlos Santonja Ion Cristóbal Miguel A Piris Socorro M Rodríguez-Pinilla |
| author_sort | Rebeca Manso |
| collection | DOAJ |
| description | Differentiating early mycosis fungoides (MF) from inflammatory dermatitis is a challenge. We compare the differential expression profile of early-stage MF samples and benign inflammatory dermatoses using microRNA (miRNA) arrays. 114 miRNAs were found to be dysregulated between these entities. The seven most differentially expressed miRNAs between these two conditions were further analyzed using RT-PCR in two series comprising 38 samples of early MFs and 18 samples of inflammatory dermatitis. A series of 51 paraffin-embedded samples belonging to paired stages of 16 MF patients was also analyzed. MiRNAs 26a, 222, 181a and 146a were differentially expressed between tumoral and inflammatory conditions. Two of these miRNAs (miRNA-181a and miRNA-146a) were significantly deregulated between early and advanced MF stages. Bioinformatic analysis showed FOXP3 expression to be regulated by these miRNAs. Immunohistochemistry revealed the level of FOXP3 expression to be lower in tumoral MFs than in plaque lesions in paraffin-embedded tissue. A functional study confirmed that both miRNAs diminished FOXP3 expression when overexpressed in CTCL cells. The data presented here suggest that the analysis of a restricted number of miRNAs (26a, 222, 181a and 146a) could be sufficient to differentiate tumoral from reactive conditions. Moreover, these miRNAs seem to be involved in MF progression. |
| format | Article |
| id | doaj-art-750299082c9f4438b8dfc7fb7e6985de |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-750299082c9f4438b8dfc7fb7e6985de2025-08-20T02:03:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01136e019847710.1371/journal.pone.0198477Mycosis fungoides progression could be regulated by microRNAs.Rebeca MansoNerea Martínez-MagunacelayaItziar Eraña-TomásVerónica MonsalvezJosé L Rodríguez-PeraltoPablo-L Ortiz-RomeroCarlos SantonjaIon CristóbalMiguel A PirisSocorro M Rodríguez-PinillaDifferentiating early mycosis fungoides (MF) from inflammatory dermatitis is a challenge. We compare the differential expression profile of early-stage MF samples and benign inflammatory dermatoses using microRNA (miRNA) arrays. 114 miRNAs were found to be dysregulated between these entities. The seven most differentially expressed miRNAs between these two conditions were further analyzed using RT-PCR in two series comprising 38 samples of early MFs and 18 samples of inflammatory dermatitis. A series of 51 paraffin-embedded samples belonging to paired stages of 16 MF patients was also analyzed. MiRNAs 26a, 222, 181a and 146a were differentially expressed between tumoral and inflammatory conditions. Two of these miRNAs (miRNA-181a and miRNA-146a) were significantly deregulated between early and advanced MF stages. Bioinformatic analysis showed FOXP3 expression to be regulated by these miRNAs. Immunohistochemistry revealed the level of FOXP3 expression to be lower in tumoral MFs than in plaque lesions in paraffin-embedded tissue. A functional study confirmed that both miRNAs diminished FOXP3 expression when overexpressed in CTCL cells. The data presented here suggest that the analysis of a restricted number of miRNAs (26a, 222, 181a and 146a) could be sufficient to differentiate tumoral from reactive conditions. Moreover, these miRNAs seem to be involved in MF progression.https://doi.org/10.1371/journal.pone.0198477 |
| spellingShingle | Rebeca Manso Nerea Martínez-Magunacelaya Itziar Eraña-Tomás Verónica Monsalvez José L Rodríguez-Peralto Pablo-L Ortiz-Romero Carlos Santonja Ion Cristóbal Miguel A Piris Socorro M Rodríguez-Pinilla Mycosis fungoides progression could be regulated by microRNAs. PLoS ONE |
| title | Mycosis fungoides progression could be regulated by microRNAs. |
| title_full | Mycosis fungoides progression could be regulated by microRNAs. |
| title_fullStr | Mycosis fungoides progression could be regulated by microRNAs. |
| title_full_unstemmed | Mycosis fungoides progression could be regulated by microRNAs. |
| title_short | Mycosis fungoides progression could be regulated by microRNAs. |
| title_sort | mycosis fungoides progression could be regulated by micrornas |
| url | https://doi.org/10.1371/journal.pone.0198477 |
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