Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw
<p>A research on screening and isolation of cellulolytic bacteria from the gut of larvae Black Soldier treated rice straw feed has been conducted. The purpose of this study is to get the type of cellulolytic bacteria from the gut of larvae and bacteria that have the highest potential to degrad...
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Universitas Negeri Semarang
2016-11-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
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| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/6762 |
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| author | Ateng Supriyatna Ukit Ukit |
| author_facet | Ateng Supriyatna Ukit Ukit |
| author_sort | Ateng Supriyatna |
| collection | DOAJ |
| description | <p>A research on screening and isolation of cellulolytic bacteria from the gut of larvae Black Soldier treated rice straw feed has been conducted. The purpose of this study is to get the type of cellulolytic bacteria from the gut of larvae and bacteria that have the highest potential to degrade cellulose. Screening and isolation method applied by using intestinal larvae obtained from larval gut vortex at a speed of 1500 rpm. Furthermore, dilution graded from 1 to 10 and grown in media CMC (carboxyl methyl cellulose) at 37 <sup>0</sup>C and incubated for 48 hours. Observations were made based on the characteristics of the microscopic, macroscopic, biochemical test, cellulolytic activity and the activity of cellulase enzymes selected bacteria. The results showed a 9 cellulolytic bacteria from the gut of the larvae. <em>Bacillus </em>sp. is a bacteria that have the highest potential with cellulolitic activity 2.1 mm (dz/dk), the exponential phase of hour at the 24<sup>th</sup>, and cellulase enzyme activity of 0.4 U/mL at pH 7 and 0.41 U/mL at pH 8.This research showed that the Black Soldier Flays Larvae (<em>Hermetia illucens</em>) have competencein organic waste degradation, because in Black Soldier Flays<em> Larvae’s</em> gut, cellulolitic enzyme is produced by cellulolitic bacteria, specially <em>Bacillus </em>sp,</p><p><strong>How to Cite</strong></p><p>Supriyatna, A., & Ukit, U. (2016). Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (<em>Hermetia illucens</em>) Feeding with Rice Straw. <em>Biosaintifika: Journal of Biology & Biology Education</em>, 8(3), 314-320. </p> |
| format | Article |
| id | doaj-art-7501bdbfc31e4ca8aa14d0bc7b01258d |
| institution | OA Journals |
| issn | 2085-191X 2338-7610 |
| language | English |
| publishDate | 2016-11-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj-art-7501bdbfc31e4ca8aa14d0bc7b01258d2025-08-20T01:57:59ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102016-11-018331432010.15294/biosaintifika.v8i3.67625202Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice StrawAteng Supriyatna0Ukit Ukit1Departement of Biology, Faculty of Science and Technology, Islamic State University of Sunan Gunung Djati Bandung, IndonesiaDepartement of Biology, Faculty of Science and Technology, Islamic State University of Sunan Gunung Djati Bandung, Indonesia<p>A research on screening and isolation of cellulolytic bacteria from the gut of larvae Black Soldier treated rice straw feed has been conducted. The purpose of this study is to get the type of cellulolytic bacteria from the gut of larvae and bacteria that have the highest potential to degrade cellulose. Screening and isolation method applied by using intestinal larvae obtained from larval gut vortex at a speed of 1500 rpm. Furthermore, dilution graded from 1 to 10 and grown in media CMC (carboxyl methyl cellulose) at 37 <sup>0</sup>C and incubated for 48 hours. Observations were made based on the characteristics of the microscopic, macroscopic, biochemical test, cellulolytic activity and the activity of cellulase enzymes selected bacteria. The results showed a 9 cellulolytic bacteria from the gut of the larvae. <em>Bacillus </em>sp. is a bacteria that have the highest potential with cellulolitic activity 2.1 mm (dz/dk), the exponential phase of hour at the 24<sup>th</sup>, and cellulase enzyme activity of 0.4 U/mL at pH 7 and 0.41 U/mL at pH 8.This research showed that the Black Soldier Flays Larvae (<em>Hermetia illucens</em>) have competencein organic waste degradation, because in Black Soldier Flays<em> Larvae’s</em> gut, cellulolitic enzyme is produced by cellulolitic bacteria, specially <em>Bacillus </em>sp,</p><p><strong>How to Cite</strong></p><p>Supriyatna, A., & Ukit, U. (2016). Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (<em>Hermetia illucens</em>) Feeding with Rice Straw. <em>Biosaintifika: Journal of Biology & Biology Education</em>, 8(3), 314-320. </p>https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/6762The enzyme activitycellulolytic bacteriaLarva Black SoldierIsolation and Screening |
| spellingShingle | Ateng Supriyatna Ukit Ukit Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw Biosaintifika: Journal of Biology & Biology Education The enzyme activity cellulolytic bacteria Larva Black Soldier Isolation and Screening |
| title | Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw |
| title_full | Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw |
| title_fullStr | Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw |
| title_full_unstemmed | Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw |
| title_short | Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw |
| title_sort | screening and isolation of cellulolytic bacteria from gut of black soldier flays larvae hermetia illucens feeding with rice straw |
| topic | The enzyme activity cellulolytic bacteria Larva Black Soldier Isolation and Screening |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/6762 |
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