Immunohistochemical evaluation of colorectal carcinoma: experience of a four-year study
Colorectal carcinomas (CRC) is the third most common cause of death in developed countries. Invoking of primary site of carcinoma of unknown origin using immunohistochemistry is essential for accurate diagnosis, and also for targeted therapies. Aim. This study aimed to assess immunohistochemical...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zaporizhzhia State Medical and Pharmaceutical University
2024-12-01
|
| Series: | Patologìâ |
| Subjects: | |
| Online Access: | http://pat.zsmu.edu.ua/article/view/312101/309603 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850251204365385728 |
|---|---|
| author | Muchalapuri Sravani Fatima Fatima Rasheed Fatima K. N. Deepthi Shilpa Karamchedu Shaikh Nishadbanu Allam Swetha |
| author_facet | Muchalapuri Sravani Fatima Fatima Rasheed Fatima K. N. Deepthi Shilpa Karamchedu Shaikh Nishadbanu Allam Swetha |
| author_sort | Muchalapuri Sravani |
| collection | DOAJ |
| description | Colorectal carcinomas (CRC) is the third most common cause of death in developed countries. Invoking of primary site of carcinoma of unknown origin using immunohistochemistry is essential for accurate diagnosis, and also for targeted therapies.
Aim. This study aimed to assess immunohistochemical expression of CK7, CK20, and CDX2 in colorectal carcinomas, and to evaluate their diagnostic role.
Materials and methods. A retrospective study was performed on 36 paraffin blocks of documented colorectal carcinomas were stained by immunohistochemical technique using a tissue microarray with CK7, CK20 and CDX2 markers. The resulted data were statistically analyzed.
Results. There was a negative association between CDX2 expression and histologic grade (p = 0.03), as well as T-pathologic stage (p = 0.01). CK7-ve / CK20+ve immune profile showed a specificity of 95 % in predicting the colorectal adenocarcinomas, which was superior to that of CDX2. CDX2 loss is related to tumour grade and depth (T-stage).
Conclusions. Both CDX2 expression, and CK7-ve / CK20+ve are the most sensitive, and specific markers to diagnose the colorectal carcinoma. CK7-ve / CK20+ve expression is used as specific marker for colorectal carcinoma for targeted therapy. |
| format | Article |
| id | doaj-art-74f9e3c2b8d442fda41446ca478e81b7 |
| institution | OA Journals |
| issn | 2306-8027 2310-1237 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Zaporizhzhia State Medical and Pharmaceutical University |
| record_format | Article |
| series | Patologìâ |
| spelling | doaj-art-74f9e3c2b8d442fda41446ca478e81b72025-08-20T01:57:59ZengZaporizhzhia State Medical and Pharmaceutical UniversityPatologìâ2306-80272310-12372024-12-01213199204https://doi.org/10.14739/2310-1237.2024.3.312101Immunohistochemical evaluation of colorectal carcinoma: experience of a four-year studyMuchalapuri Sravani0https://orcid.org/0009-0008-1592-7470Fatima Fatima1https://orcid.org/0000-0001-8605-3821Rasheed Fatima2https://orcid.org/0000-0002-1446-1030K. N. Deepthi3https://orcid.org/0000-0002-1974-4398Shilpa Karamchedu4https://orcid.org/0000-0002-8074-4338Shaikh Nishadbanu5https://orcid.org/0000-0002-9711-018XAllam Swetha6https://orcid.org/0009-0004-8549-056XSVS Medical College and Hospital, Yenugonda, Mahabubnagar, Telangana, IndiaOsmania Medical College, Koti, Hyderabad, Telangana, IndiaSVS Medical College and Hospital, Yenugonda, Mahabubnagar, Telangana, IndiaSVS Medical College and Hospital, Yenugonda, Mahabubnagar, Telangana, IndiaSVS Medical College and Hospital, Yenugonda, Mahabubnagar, Telangana, IndiaSVS Medical College and Hospital, Yenugonda, Mahabubnagar, Telangana, IndiaSVS Medical College and Hospital, Yenugonda, Mahabubnagar, Telangana, IndiaColorectal carcinomas (CRC) is the third most common cause of death in developed countries. Invoking of primary site of carcinoma of unknown origin using immunohistochemistry is essential for accurate diagnosis, and also for targeted therapies. Aim. This study aimed to assess immunohistochemical expression of CK7, CK20, and CDX2 in colorectal carcinomas, and to evaluate their diagnostic role. Materials and methods. A retrospective study was performed on 36 paraffin blocks of documented colorectal carcinomas were stained by immunohistochemical technique using a tissue microarray with CK7, CK20 and CDX2 markers. The resulted data were statistically analyzed. Results. There was a negative association between CDX2 expression and histologic grade (p = 0.03), as well as T-pathologic stage (p = 0.01). CK7-ve / CK20+ve immune profile showed a specificity of 95 % in predicting the colorectal adenocarcinomas, which was superior to that of CDX2. CDX2 loss is related to tumour grade and depth (T-stage). Conclusions. Both CDX2 expression, and CK7-ve / CK20+ve are the most sensitive, and specific markers to diagnose the colorectal carcinoma. CK7-ve / CK20+ve expression is used as specific marker for colorectal carcinoma for targeted therapy.http://pat.zsmu.edu.ua/article/view/312101/309603colorectal carcinomaimmunohistochemistrycdx2ck7ck20 |
| spellingShingle | Muchalapuri Sravani Fatima Fatima Rasheed Fatima K. N. Deepthi Shilpa Karamchedu Shaikh Nishadbanu Allam Swetha Immunohistochemical evaluation of colorectal carcinoma: experience of a four-year study Patologìâ colorectal carcinoma immunohistochemistry cdx2 ck7 ck20 |
| title | Immunohistochemical evaluation of colorectal carcinoma: experience of a four-year study |
| title_full | Immunohistochemical evaluation of colorectal carcinoma: experience of a four-year study |
| title_fullStr | Immunohistochemical evaluation of colorectal carcinoma: experience of a four-year study |
| title_full_unstemmed | Immunohistochemical evaluation of colorectal carcinoma: experience of a four-year study |
| title_short | Immunohistochemical evaluation of colorectal carcinoma: experience of a four-year study |
| title_sort | immunohistochemical evaluation of colorectal carcinoma experience of a four year study |
| topic | colorectal carcinoma immunohistochemistry cdx2 ck7 ck20 |
| url | http://pat.zsmu.edu.ua/article/view/312101/309603 |
| work_keys_str_mv | AT muchalapurisravani immunohistochemicalevaluationofcolorectalcarcinomaexperienceofafouryearstudy AT fatimafatima immunohistochemicalevaluationofcolorectalcarcinomaexperienceofafouryearstudy AT rasheedfatima immunohistochemicalevaluationofcolorectalcarcinomaexperienceofafouryearstudy AT kndeepthi immunohistochemicalevaluationofcolorectalcarcinomaexperienceofafouryearstudy AT shilpakaramchedu immunohistochemicalevaluationofcolorectalcarcinomaexperienceofafouryearstudy AT shaikhnishadbanu immunohistochemicalevaluationofcolorectalcarcinomaexperienceofafouryearstudy AT allamswetha immunohistochemicalevaluationofcolorectalcarcinomaexperienceofafouryearstudy |