Metabolomic Analysis of <i>Elymus sibiricus</i> Exposed to UV-B Radiation Stress
Plants cultivated on the Qinghai-Tibet Plateau (QTP) are exposed to high ultraviolet radiation intensities, so they require effective mechanisms to adapt to these stress conditions. UV-B radiation is an abiotic stress factor that affects plant growth, development, and environmental adaptation. <i...
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2024-10-01
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| author | Fei Zhang Ming Sun Daxu Li Minghong You Jiajun Yan Shiqie Bai |
| author_facet | Fei Zhang Ming Sun Daxu Li Minghong You Jiajun Yan Shiqie Bai |
| author_sort | Fei Zhang |
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| description | Plants cultivated on the Qinghai-Tibet Plateau (QTP) are exposed to high ultraviolet radiation intensities, so they require effective mechanisms to adapt to these stress conditions. UV-B radiation is an abiotic stress factor that affects plant growth, development, and environmental adaptation. <i>Elymus sibiricus</i> is a common species in the alpine meadows of the QTP, with high-stress resistance, large biomass, and high nutritional value. This species plays an important role in establishing artificial grasslands and improving degraded grasslands. In this study, UV-B radiation-tolerant and UV-B radiation-sensitive <i>E. sibiricus</i> genotypes were subjected to simulated short-term (5 days, 10 days) and long-term (15 days, 20 days) UV-B radiation stress and the metabolite profiles evaluated to explore the mechanism underlying UV-B radiation resistance in <i>E. sibiricus</i>. A total of 699 metabolites were identified, including 11 primary metabolites such as lipids and lipid-like molecules, phenylpropanoids and polyketides, organic acids and their derivatives, and organic oxygen compounds. Principal component analysis distinctly clustered the samples according to the cultivar, indicating that the two genotypes exhibit distinct response mechanisms to UV-B radiation stress. The results showed that 14 metabolites, including linoleic acid, LPC 18:2, xanthosine, and 23 metabolites, including 2-one heptamethoxyflavone, glycyrrhizin, and caffeic acid were differentially expressed under short-term and long-term UV-B radiation stress, respectively. Therefore, these compounds are potential biomarkers for evaluating <i>E. sibiricus</i> response to UV-B radiation stress. Allantoin specific and consistent expression was up-regulated in the UV-B radiation-tolerant genotype, thereby it can be used to identify varieties resistant to UV-B radiation. Different metabolic profiles and UV-B radiation response mechanisms were observed between the UV-B radiation-tolerant and UV-B radiation-sensitive <i>E. sibiricus</i> genotypes. A model for the metabolic pathways and metabolic profiles was constructed for the two genotypes. This metabolomic study on the <i>E. sibiricus</i> response to UV-B radiation stress provides a reference for the breeding of new UV-B radiation-tolerant <i>E. sibiricus</i> cultivars. |
| format | Article |
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| institution | DOAJ |
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| spelling | doaj-art-74f9a68c25a44faaa7705359a8b7c02b2025-08-20T02:49:55ZengMDPI AGMolecules1420-30492024-10-012921513310.3390/molecules29215133Metabolomic Analysis of <i>Elymus sibiricus</i> Exposed to UV-B Radiation StressFei Zhang0Ming Sun1Daxu Li2Minghong You3Jiajun Yan4Shiqie Bai5College of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaCollege of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSichuan Provincial Forestry and Glassland Key Laboratory of Innovation and Utilization of Grasses in the Tibetan Plateau, Sichuan Academy of Grassland Sciences, Chengdu 611731, ChinaSichuan Provincial Forestry and Glassland Key Laboratory of Innovation and Utilization of Grasses in the Tibetan Plateau, Sichuan Academy of Grassland Sciences, Chengdu 611731, ChinaCollege of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaCollege of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaPlants cultivated on the Qinghai-Tibet Plateau (QTP) are exposed to high ultraviolet radiation intensities, so they require effective mechanisms to adapt to these stress conditions. UV-B radiation is an abiotic stress factor that affects plant growth, development, and environmental adaptation. <i>Elymus sibiricus</i> is a common species in the alpine meadows of the QTP, with high-stress resistance, large biomass, and high nutritional value. This species plays an important role in establishing artificial grasslands and improving degraded grasslands. In this study, UV-B radiation-tolerant and UV-B radiation-sensitive <i>E. sibiricus</i> genotypes were subjected to simulated short-term (5 days, 10 days) and long-term (15 days, 20 days) UV-B radiation stress and the metabolite profiles evaluated to explore the mechanism underlying UV-B radiation resistance in <i>E. sibiricus</i>. A total of 699 metabolites were identified, including 11 primary metabolites such as lipids and lipid-like molecules, phenylpropanoids and polyketides, organic acids and their derivatives, and organic oxygen compounds. Principal component analysis distinctly clustered the samples according to the cultivar, indicating that the two genotypes exhibit distinct response mechanisms to UV-B radiation stress. The results showed that 14 metabolites, including linoleic acid, LPC 18:2, xanthosine, and 23 metabolites, including 2-one heptamethoxyflavone, glycyrrhizin, and caffeic acid were differentially expressed under short-term and long-term UV-B radiation stress, respectively. Therefore, these compounds are potential biomarkers for evaluating <i>E. sibiricus</i> response to UV-B radiation stress. Allantoin specific and consistent expression was up-regulated in the UV-B radiation-tolerant genotype, thereby it can be used to identify varieties resistant to UV-B radiation. Different metabolic profiles and UV-B radiation response mechanisms were observed between the UV-B radiation-tolerant and UV-B radiation-sensitive <i>E. sibiricus</i> genotypes. A model for the metabolic pathways and metabolic profiles was constructed for the two genotypes. This metabolomic study on the <i>E. sibiricus</i> response to UV-B radiation stress provides a reference for the breeding of new UV-B radiation-tolerant <i>E. sibiricus</i> cultivars.https://www.mdpi.com/1420-3049/29/21/5133<i>Elymus sibiricus</i>metabolomicsUV-B radiation stressallantoin |
| spellingShingle | Fei Zhang Ming Sun Daxu Li Minghong You Jiajun Yan Shiqie Bai Metabolomic Analysis of <i>Elymus sibiricus</i> Exposed to UV-B Radiation Stress Molecules <i>Elymus sibiricus</i> metabolomics UV-B radiation stress allantoin |
| title | Metabolomic Analysis of <i>Elymus sibiricus</i> Exposed to UV-B Radiation Stress |
| title_full | Metabolomic Analysis of <i>Elymus sibiricus</i> Exposed to UV-B Radiation Stress |
| title_fullStr | Metabolomic Analysis of <i>Elymus sibiricus</i> Exposed to UV-B Radiation Stress |
| title_full_unstemmed | Metabolomic Analysis of <i>Elymus sibiricus</i> Exposed to UV-B Radiation Stress |
| title_short | Metabolomic Analysis of <i>Elymus sibiricus</i> Exposed to UV-B Radiation Stress |
| title_sort | metabolomic analysis of i elymus sibiricus i exposed to uv b radiation stress |
| topic | <i>Elymus sibiricus</i> metabolomics UV-B radiation stress allantoin |
| url | https://www.mdpi.com/1420-3049/29/21/5133 |
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