Chromosome-level genome assembly of the lemon sole, Microstomus kitt (Pleuronectiformes: Pleuronectidae)
Background The lemon sole (Microstomus kitt) is a culinary fish from the family of righteye flounders (Pleuronectidae), inhabiting sandy, shallow offshore grounds of the North Sea, western Baltic Sea, English Channel, Great Britain and Ireland, Bay of Biscay, and coastal waters of Norway. Findin...
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GigaScience Press
2025-05-01
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| Online Access: | https://gigabytejournal.com/articles/156 |
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| author | Marcel Nebenführ David Prochotta Maria A. Nilsson Menno J. de Jong Tunca D. Yazici Fabienne Langefeld Malambo Muloongo Helena Woköck Jakob Jilg Sina C. Bender Marvin M. Zangl Juan-Manuel Ortega Guatame Kimberley Williams Moritz Sonnewald Axel Janke |
| author_facet | Marcel Nebenführ David Prochotta Maria A. Nilsson Menno J. de Jong Tunca D. Yazici Fabienne Langefeld Malambo Muloongo Helena Woköck Jakob Jilg Sina C. Bender Marvin M. Zangl Juan-Manuel Ortega Guatame Kimberley Williams Moritz Sonnewald Axel Janke |
| author_sort | Marcel Nebenführ |
| collection | DOAJ |
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Background
The lemon sole (Microstomus kitt) is a culinary fish from the family of righteye flounders (Pleuronectidae), inhabiting sandy, shallow offshore grounds of the North Sea, western Baltic Sea, English Channel, Great Britain and Ireland, Bay of Biscay, and coastal waters of Norway.
Findings
Here, we present a chromosome-level genome assembly of the lemon sole. We applied PacBio HiFi sequencing on the PacBio Revio system to generate a highly complete and contiguous reference genome.
The resulting assembly has a contig N50 of 17.2 Mbp and a scaffold N50 of 27.2 Mbp. The total assembly length is 628 Mbp, comprising 24 chromosome-length scaffolds. The identification of 99.7% complete BUSCO genes indicates a high level of assembly completeness.
Conclusions
The chromosome-level genome assembly of the lemon sole provides a high-quality reference genome for future population-level genomic analyses of this commercially valuable, edible fish.
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| format | Article |
| id | doaj-art-74c860a8a2ca4065ac0da9f0b3e0ab28 |
| institution | OA Journals |
| issn | 2709-4715 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | GigaScience Press |
| record_format | Article |
| series | GigaByte |
| spelling | doaj-art-74c860a8a2ca4065ac0da9f0b3e0ab282025-08-20T01:57:16ZengGigaScience PressGigaByte2709-47152025-05-0110.46471/gigabyte.156Chromosome-level genome assembly of the lemon sole, Microstomus kitt (Pleuronectiformes: Pleuronectidae)Marcel Nebenführ 0https://orcid.org/0000-0001-8802-2105David Prochotta 1https://orcid.org/0009-0000-6275-7752Maria A. Nilsson 2https://orcid.org/0000-0002-8136-7263Menno J. de Jong 3https://orcid.org/0000-0003-2131-9048Tunca D. Yazici 4https://orcid.org/0009-0007-4341-3824Fabienne Langefeld 5https://orcid.org/0009-0003-8523-4665Malambo Muloongo 6https://orcid.org/0000-0003-0220-1474Helena Woköck 7https://orcid.org/0009-0000-3322-1780Jakob Jilg 8https://orcid.org/0000-0002-9910-4146Sina C. Bender 9https://orcid.org/0009-0002-5800-5384Marvin M. Zangl 10https://orcid.org/0009-0003-8108-9181Juan-Manuel Ortega Guatame 11https://orcid.org/0009-0009-8231-6191Kimberley Williams 12https://orcid.org/0009-0008-9521-0989Moritz Sonnewald 13https://orcid.org/0000-0003-3042-8107Axel Janke 14https://orcid.org/0000-0002-9394-1904https://ror.org/01amp2a31Senckenberg Biodiversity and Climate Research Centre (BiK-F), Frankfurt am Main, Germany, Institute for Ecology, Evolution, and Diversity, https://ror.org/04cvxnb49Goethe University, Frankfurt am Main, Germany, https://ror.org/0396gab88LOEWE-Centre for Translational Biodiversity Genomics (TBG), Frankfurt am Main, Germanyhttps://ror.org/01amp2a31Senckenberg Biodiversity and Climate Research Centre (BiK-F), Frankfurt am Main, Germany, Institute for Ecology, Evolution, and Diversity, https://ror.org/04cvxnb49Goethe University, Frankfurt am Main, Germany, https://ror.org/0396gab88LOEWE-Centre for Translational Biodiversity Genomics (TBG), Frankfurt am Main, Germanyhttps://ror.org/01amp2a31Senckenberg Biodiversity and Climate Research Centre (BiK-F), Frankfurt am Main, Germany, Institute for Ecology, Evolution, and Diversity, https://ror.org/04cvxnb49Goethe University, Frankfurt am Main, Germany, https://ror.org/0396gab88LOEWE-Centre for Translational Biodiversity Genomics (TBG), Frankfurt am Main, Germanyhttps://ror.org/01amp2a31Senckenberg Biodiversity and Climate Research Centre (BiK-F), Frankfurt am Main, Germany, Institute for Ecology, Evolution, and Diversity, https://ror.org/04cvxnb49Goethe University, Frankfurt am Main, Germany, https://ror.org/0396gab88LOEWE-Centre for Translational Biodiversity Genomics (TBG), Frankfurt am Main, Germanyhttps://ror.org/01amp2a31Senckenberg Biodiversity and Climate Research Centre (BiK-F), Frankfurt am Main, Germany, Institute for Ecology, Evolution, and Diversity, https://ror.org/04cvxnb49Goethe University, Frankfurt am Main, Germany, https://ror.org/0396gab88LOEWE-Centre for Translational Biodiversity Genomics (TBG), Frankfurt am Main, GermanyInstitute for Ecology, Evolution, and Diversity, https://ror.org/04cvxnb49Goethe University, Frankfurt am Main, GermanyDepartment of Aquaculture & Fisheries Management, https://ror.org/03wx2rr30University of Ibadan, NigeriaInstitute for Ecology, Evolution, and Diversity, https://ror.org/04cvxnb49Goethe University, Frankfurt am Main, GermanyInstitute for Ecology, Evolution, and Diversity, https://ror.org/04cvxnb49Goethe University, Frankfurt am Main, GermanyInstitute for Ecology, Evolution, and Diversity, https://ror.org/04cvxnb49Goethe University, Frankfurt am Main, GermanyInstitute for Ecology, Evolution, and Diversity, https://ror.org/04cvxnb49Goethe University, Frankfurt am Main, Germanyhttps://ror.org/0396gab88LOEWE-Centre for Translational Biodiversity Genomics (TBG), Frankfurt am Main, Germany, Centre for Wildlife Genetics, https://ror.org/01wz97s39Senckenberg Research Institute and Natural History Museum, Gelnhausen, GermanyInstitute for Ecology, Evolution, and Diversity, https://ror.org/04cvxnb49Goethe University, Frankfurt am Main, GermanySenckenberg Research Institute, Department of Marine Zoology, Section Ichthyology, Frankfurt am Main, Germanyhttps://ror.org/01amp2a31Senckenberg Biodiversity and Climate Research Centre (BiK-F), Frankfurt am Main, Germany, Institute for Ecology, Evolution, and Diversity, https://ror.org/04cvxnb49Goethe University, Frankfurt am Main, Germany, https://ror.org/0396gab88LOEWE-Centre for Translational Biodiversity Genomics (TBG), Frankfurt am Main, Germany Background The lemon sole (Microstomus kitt) is a culinary fish from the family of righteye flounders (Pleuronectidae), inhabiting sandy, shallow offshore grounds of the North Sea, western Baltic Sea, English Channel, Great Britain and Ireland, Bay of Biscay, and coastal waters of Norway. Findings Here, we present a chromosome-level genome assembly of the lemon sole. We applied PacBio HiFi sequencing on the PacBio Revio system to generate a highly complete and contiguous reference genome. The resulting assembly has a contig N50 of 17.2 Mbp and a scaffold N50 of 27.2 Mbp. The total assembly length is 628 Mbp, comprising 24 chromosome-length scaffolds. The identification of 99.7% complete BUSCO genes indicates a high level of assembly completeness. Conclusions The chromosome-level genome assembly of the lemon sole provides a high-quality reference genome for future population-level genomic analyses of this commercially valuable, edible fish. https://gigabytejournal.com/articles/156 |
| spellingShingle | Marcel Nebenführ David Prochotta Maria A. Nilsson Menno J. de Jong Tunca D. Yazici Fabienne Langefeld Malambo Muloongo Helena Woköck Jakob Jilg Sina C. Bender Marvin M. Zangl Juan-Manuel Ortega Guatame Kimberley Williams Moritz Sonnewald Axel Janke Chromosome-level genome assembly of the lemon sole, Microstomus kitt (Pleuronectiformes: Pleuronectidae) GigaByte |
| title | Chromosome-level genome assembly of the lemon sole, Microstomus kitt (Pleuronectiformes: Pleuronectidae) |
| title_full | Chromosome-level genome assembly of the lemon sole, Microstomus kitt (Pleuronectiformes: Pleuronectidae) |
| title_fullStr | Chromosome-level genome assembly of the lemon sole, Microstomus kitt (Pleuronectiformes: Pleuronectidae) |
| title_full_unstemmed | Chromosome-level genome assembly of the lemon sole, Microstomus kitt (Pleuronectiformes: Pleuronectidae) |
| title_short | Chromosome-level genome assembly of the lemon sole, Microstomus kitt (Pleuronectiformes: Pleuronectidae) |
| title_sort | chromosome level genome assembly of the lemon sole microstomus kitt pleuronectiformes pleuronectidae |
| url | https://gigabytejournal.com/articles/156 |
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