Quasispecies Changes with Distinctive Point Mutations in the Hepatitis C Virus Internal Ribosome Entry Site (IRES) Derived from PBMCs and Plasma
The 5’ untranslated region (UTR) of the hepatitis C virus (HCV) genome contains the internal ribosome entry site (IRES), a highly conserved RNA structure essential for cap-independent translation of the viral polyprotein. HCV, apart from the liver, is thought to be associated with lymphocyte subpopu...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2018/4835252 |
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| author | Luca Mercuri Emma C. Thomson Joseph Hughes Peter Karayiannis |
| author_facet | Luca Mercuri Emma C. Thomson Joseph Hughes Peter Karayiannis |
| author_sort | Luca Mercuri |
| collection | DOAJ |
| description | The 5’ untranslated region (UTR) of the hepatitis C virus (HCV) genome contains the internal ribosome entry site (IRES), a highly conserved RNA structure essential for cap-independent translation of the viral polyprotein. HCV, apart from the liver, is thought to be associated with lymphocyte subpopulations of peripheral blood mononuclear cells (PBMCs), in lymph nodes and brain tissue. In this study, RT-PCR, cloning, and sequence analysis were employed to investigate the quasispecies nature of the 5’UTR following extraction of viral RNA from PBMCs and plasma of HCV infected individuals. The nucleotide variation between IRES-derived sequences from PBMCs and plasma indicated the existence of polymorphic sites within the IRES. HCV isolates had divergent variants with unique mutations particularly at positions 107, 204, and 243 of the IRES. Most of the PBMC-derived sequences contained an A-A-A variant at these positions. The mutations associated with the IRESes suggested the presence of unique quasispecies populations in PBMCs compared with plasma. |
| format | Article |
| id | doaj-art-74c734b9d4064cc3b49a335bb3121d7d |
| institution | Kabale University |
| issn | 1687-8639 1687-8647 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-74c734b9d4064cc3b49a335bb3121d7d2025-08-20T03:34:09ZengWileyAdvances in Virology1687-86391687-86472018-01-01201810.1155/2018/48352524835252Quasispecies Changes with Distinctive Point Mutations in the Hepatitis C Virus Internal Ribosome Entry Site (IRES) Derived from PBMCs and PlasmaLuca Mercuri0Emma C. Thomson1Joseph Hughes2Peter Karayiannis3Hepatology Section, Division of Medicine, Faculty of Medicine, Imperial College, London, UKUniversity of Glasgow MRC Centre for Virus Research, Glasgow, UKUniversity of Glasgow MRC Centre for Virus Research, Glasgow, UKUniversity of Nicosia Medical School, Nicosia, CyprusThe 5’ untranslated region (UTR) of the hepatitis C virus (HCV) genome contains the internal ribosome entry site (IRES), a highly conserved RNA structure essential for cap-independent translation of the viral polyprotein. HCV, apart from the liver, is thought to be associated with lymphocyte subpopulations of peripheral blood mononuclear cells (PBMCs), in lymph nodes and brain tissue. In this study, RT-PCR, cloning, and sequence analysis were employed to investigate the quasispecies nature of the 5’UTR following extraction of viral RNA from PBMCs and plasma of HCV infected individuals. The nucleotide variation between IRES-derived sequences from PBMCs and plasma indicated the existence of polymorphic sites within the IRES. HCV isolates had divergent variants with unique mutations particularly at positions 107, 204, and 243 of the IRES. Most of the PBMC-derived sequences contained an A-A-A variant at these positions. The mutations associated with the IRESes suggested the presence of unique quasispecies populations in PBMCs compared with plasma.http://dx.doi.org/10.1155/2018/4835252 |
| spellingShingle | Luca Mercuri Emma C. Thomson Joseph Hughes Peter Karayiannis Quasispecies Changes with Distinctive Point Mutations in the Hepatitis C Virus Internal Ribosome Entry Site (IRES) Derived from PBMCs and Plasma Advances in Virology |
| title | Quasispecies Changes with Distinctive Point Mutations in the Hepatitis C Virus Internal Ribosome Entry Site (IRES) Derived from PBMCs and Plasma |
| title_full | Quasispecies Changes with Distinctive Point Mutations in the Hepatitis C Virus Internal Ribosome Entry Site (IRES) Derived from PBMCs and Plasma |
| title_fullStr | Quasispecies Changes with Distinctive Point Mutations in the Hepatitis C Virus Internal Ribosome Entry Site (IRES) Derived from PBMCs and Plasma |
| title_full_unstemmed | Quasispecies Changes with Distinctive Point Mutations in the Hepatitis C Virus Internal Ribosome Entry Site (IRES) Derived from PBMCs and Plasma |
| title_short | Quasispecies Changes with Distinctive Point Mutations in the Hepatitis C Virus Internal Ribosome Entry Site (IRES) Derived from PBMCs and Plasma |
| title_sort | quasispecies changes with distinctive point mutations in the hepatitis c virus internal ribosome entry site ires derived from pbmcs and plasma |
| url | http://dx.doi.org/10.1155/2018/4835252 |
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