In vitro protein digestibility and bioactive activities of yogurt from black soybean (Glycine max L.) milk

Black soybean raw material has high nutritional value and shows potential for producing plant-based yogurt alternatives. In this study, black soybean milk was fermented with a commercial yogurt starter to produce black soybean yogurt. The chemical composition, bioactive activities, amino acid profil...

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Main Authors: Thidarat Pantoa, Kanthida Wadeesirisak, Safiah Saah, Pramuan Saithong
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Future Foods
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S266683352500098X
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author Thidarat Pantoa
Kanthida Wadeesirisak
Safiah Saah
Pramuan Saithong
author_facet Thidarat Pantoa
Kanthida Wadeesirisak
Safiah Saah
Pramuan Saithong
author_sort Thidarat Pantoa
collection DOAJ
description Black soybean raw material has high nutritional value and shows potential for producing plant-based yogurt alternatives. In this study, black soybean milk was fermented with a commercial yogurt starter to produce black soybean yogurt. The chemical composition, bioactive activities, amino acid profile, protein digestibility, release of free amino acids during in vitro digestion, and bioactive activities after in vitro digestion of black soybean yogurt and black soybean milk were compared. Fermentation led to a significant decrease in carbohydrate content (94.69 %db) while increasing protein (3.45 % db), fat (1.55 % db), and ash contents (0.30 % db). Black soybean yogurt exhibited higher α-amylase (1.26 mg acarbose/mL), α-glucosidase (31.33 mg acarbose/mL), and ACE inhibitory activity (97.10 %) than unfermented black soybean milk, with improved protein digestibility and higher release of free amino acids during in vitro digestion. The increased digestibility of black soybean yogurt contributed to higher bioactive activity in the digesta after the duodenal phase. Black soybean yogurt displays strong potential in the plant-based yogurt market due to its high nutritional value, complete amino acid profile, easy digestibility, and potent bioactive properties.
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publisher Elsevier
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spelling doaj-art-74ba21fcc9834794aaf64a1fe4a8780d2025-08-20T03:09:44ZengElsevierFuture Foods2666-83352025-06-011110063610.1016/j.fufo.2025.100636In vitro protein digestibility and bioactive activities of yogurt from black soybean (Glycine max L.) milkThidarat Pantoa0Kanthida Wadeesirisak1Safiah Saah2Pramuan Saithong3Department of Food Chemistry and Physics, Institute of Food Research and Product Development, Kasetsart University, Chatuchak, Bangkok 10900, Thailand; Corresponding author.Department of Applied Microbiology, Institute of Food Research and Product Development, Kasetsart University, Chatuchak, Bangkok 10900, ThailandDepartment of Nutrition and Health, Institute of Food Research and Product Development, Kasetsart University, Chatuchak, Bangkok 10900, ThailandDepartment of Applied Microbiology, Institute of Food Research and Product Development, Kasetsart University, Chatuchak, Bangkok 10900, ThailandBlack soybean raw material has high nutritional value and shows potential for producing plant-based yogurt alternatives. In this study, black soybean milk was fermented with a commercial yogurt starter to produce black soybean yogurt. The chemical composition, bioactive activities, amino acid profile, protein digestibility, release of free amino acids during in vitro digestion, and bioactive activities after in vitro digestion of black soybean yogurt and black soybean milk were compared. Fermentation led to a significant decrease in carbohydrate content (94.69 %db) while increasing protein (3.45 % db), fat (1.55 % db), and ash contents (0.30 % db). Black soybean yogurt exhibited higher α-amylase (1.26 mg acarbose/mL), α-glucosidase (31.33 mg acarbose/mL), and ACE inhibitory activity (97.10 %) than unfermented black soybean milk, with improved protein digestibility and higher release of free amino acids during in vitro digestion. The increased digestibility of black soybean yogurt contributed to higher bioactive activity in the digesta after the duodenal phase. Black soybean yogurt displays strong potential in the plant-based yogurt market due to its high nutritional value, complete amino acid profile, easy digestibility, and potent bioactive properties.http://www.sciencedirect.com/science/article/pii/S266683352500098XBlack soybeanBioactive activityProtein digestibilityYogurtPlant-based
spellingShingle Thidarat Pantoa
Kanthida Wadeesirisak
Safiah Saah
Pramuan Saithong
In vitro protein digestibility and bioactive activities of yogurt from black soybean (Glycine max L.) milk
Future Foods
Black soybean
Bioactive activity
Protein digestibility
Yogurt
Plant-based
title In vitro protein digestibility and bioactive activities of yogurt from black soybean (Glycine max L.) milk
title_full In vitro protein digestibility and bioactive activities of yogurt from black soybean (Glycine max L.) milk
title_fullStr In vitro protein digestibility and bioactive activities of yogurt from black soybean (Glycine max L.) milk
title_full_unstemmed In vitro protein digestibility and bioactive activities of yogurt from black soybean (Glycine max L.) milk
title_short In vitro protein digestibility and bioactive activities of yogurt from black soybean (Glycine max L.) milk
title_sort in vitro protein digestibility and bioactive activities of yogurt from black soybean glycine max l milk
topic Black soybean
Bioactive activity
Protein digestibility
Yogurt
Plant-based
url http://www.sciencedirect.com/science/article/pii/S266683352500098X
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