Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status.
The type V intermediate filament lamins are the principal components of the nuclear matrix, including the nuclear lamina. Lamins are divided into A-type and B-type, which are encoded by three genes, LMNA, LMNB1, and LMNB2. The alternative splicing of LMNA produces two major A-type lamins, lamin A an...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183136&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850229074695290880 |
|---|---|
| author | Elise Kaspi Diane Frankel Julien Guinde Sophie Perrin Sophie Laroumagne Andrée Robaglia-Schlupp Kevin Ostacolo Karim Harhouri Rachid Tazi-Mezalek Joelle Micallef Hervé Dutau Pascale Tomasini Annachiara De Sandre-Giovannoli Nicolas Lévy Pierre Cau Philippe Astoul Patrice Roll |
| author_facet | Elise Kaspi Diane Frankel Julien Guinde Sophie Perrin Sophie Laroumagne Andrée Robaglia-Schlupp Kevin Ostacolo Karim Harhouri Rachid Tazi-Mezalek Joelle Micallef Hervé Dutau Pascale Tomasini Annachiara De Sandre-Giovannoli Nicolas Lévy Pierre Cau Philippe Astoul Patrice Roll |
| author_sort | Elise Kaspi |
| collection | DOAJ |
| description | The type V intermediate filament lamins are the principal components of the nuclear matrix, including the nuclear lamina. Lamins are divided into A-type and B-type, which are encoded by three genes, LMNA, LMNB1, and LMNB2. The alternative splicing of LMNA produces two major A-type lamins, lamin A and lamin C. Previous studies have suggested that lamins are involved in cancer development and progression. A-type lamins have been proposed as biomarkers for cancer diagnosis, prognosis, and/or follow-up. The aim of the present study was to investigate lamins in cancer cells from metastatic pleural effusions using immunofluorescence, western blotting, and flow cytometry. In a sub-group of lung adenocarcinomas, we found reduced expression of lamin A but not of lamin C. The reduction in lamin A expression was correlated with the loss of epithelial membrane antigen (EMA)/MUC-1, an epithelial marker that is involved in the epithelial to mesenchymal transition (EMT). Finally, the lamin A expression was inversely correlated with the number of metastatic sites and the WHO Performance status, and association of pleural, bone and lung metastatic localizations was more frequent when lamin A expression was reduced. In conclusion, low lamin A but not lamin C expression in pleural metastatic cells could represent a major actor in the development of metastasis, associated with EMT and could account for a pejorative factor correlated with a poor Performance status. |
| format | Article |
| id | doaj-art-74b656504dfd40b5abb9c1966b701d0e |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-74b656504dfd40b5abb9c1966b701d0e2025-08-20T02:04:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018313610.1371/journal.pone.0183136Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status.Elise KaspiDiane FrankelJulien GuindeSophie PerrinSophie LaroumagneAndrée Robaglia-SchluppKevin OstacoloKarim HarhouriRachid Tazi-MezalekJoelle MicallefHervé DutauPascale TomasiniAnnachiara De Sandre-GiovannoliNicolas LévyPierre CauPhilippe AstoulPatrice RollThe type V intermediate filament lamins are the principal components of the nuclear matrix, including the nuclear lamina. Lamins are divided into A-type and B-type, which are encoded by three genes, LMNA, LMNB1, and LMNB2. The alternative splicing of LMNA produces two major A-type lamins, lamin A and lamin C. Previous studies have suggested that lamins are involved in cancer development and progression. A-type lamins have been proposed as biomarkers for cancer diagnosis, prognosis, and/or follow-up. The aim of the present study was to investigate lamins in cancer cells from metastatic pleural effusions using immunofluorescence, western blotting, and flow cytometry. In a sub-group of lung adenocarcinomas, we found reduced expression of lamin A but not of lamin C. The reduction in lamin A expression was correlated with the loss of epithelial membrane antigen (EMA)/MUC-1, an epithelial marker that is involved in the epithelial to mesenchymal transition (EMT). Finally, the lamin A expression was inversely correlated with the number of metastatic sites and the WHO Performance status, and association of pleural, bone and lung metastatic localizations was more frequent when lamin A expression was reduced. In conclusion, low lamin A but not lamin C expression in pleural metastatic cells could represent a major actor in the development of metastasis, associated with EMT and could account for a pejorative factor correlated with a poor Performance status.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183136&type=printable |
| spellingShingle | Elise Kaspi Diane Frankel Julien Guinde Sophie Perrin Sophie Laroumagne Andrée Robaglia-Schlupp Kevin Ostacolo Karim Harhouri Rachid Tazi-Mezalek Joelle Micallef Hervé Dutau Pascale Tomasini Annachiara De Sandre-Giovannoli Nicolas Lévy Pierre Cau Philippe Astoul Patrice Roll Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status. PLoS ONE |
| title | Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status. |
| title_full | Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status. |
| title_fullStr | Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status. |
| title_full_unstemmed | Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status. |
| title_short | Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status. |
| title_sort | low lamin a expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor performance status |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183136&type=printable |
| work_keys_str_mv | AT elisekaspi lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT dianefrankel lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT julienguinde lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT sophieperrin lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT sophielaroumagne lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT andreerobagliaschlupp lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT kevinostacolo lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT karimharhouri lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT rachidtazimezalek lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT joellemicallef lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT hervedutau lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT pascaletomasini lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT annachiaradesandregiovannoli lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT nicolaslevy lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT pierrecau lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT philippeastoul lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus AT patriceroll lowlaminaexpressioninlungadenocarcinomacellsfrompleuraleffusionsisapejorativefactorassociatedwithhighnumberofmetastaticsitesandpoorperformancestatus |