Molecular detection and characterization of infectious bronchitis virus in live bird markets in Jos, Plateau State, Nigeria
Infectious Bronchitis Virus (IBV) is a highly contagious coronavirus of chickens, ducks, turkeys and Pheasants. It mostly affects the respiratory, urinary and reproductive tracts, leading to considerable economic losses in the poultry industry as a result of drop in egg production, undesirable egg q...
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Nigerian Academy of Science
2025-06-01
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| Series: | The Proceedings of the Nigerian Academy of Science |
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| Online Access: | https://nasjournal.org.ng/site/index.php/pnas/article/view/694/371 |
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| author | Bitrus Inuwa Hassan I. Musa Hanbali I. Umar Abdulyekin O. Tijani Shuaibu G. Adamu Muhammed A. Sodiq Ismaila Shittu Clement Meseko Tony Joannis |
| author_facet | Bitrus Inuwa Hassan I. Musa Hanbali I. Umar Abdulyekin O. Tijani Shuaibu G. Adamu Muhammed A. Sodiq Ismaila Shittu Clement Meseko Tony Joannis |
| author_sort | Bitrus Inuwa |
| collection | DOAJ |
| description | Infectious Bronchitis Virus (IBV) is a highly contagious coronavirus of chickens, ducks, turkeys and Pheasants. It mostly affects the respiratory, urinary and reproductive tracts, leading to considerable economic losses in the poultry industry as a result of drop in egg production, undesirable egg quality and poor weight gain. Thus, this study was designed to detect and characterize IBV circulating in live bird markets (LBMs) in Plateau State, Nigeria. One hundred pools of tracheal and cloacal swabs each were collected from two LBMs in Jos. Viral RNA was extracted and screened for IBV using real-time RT-PCR. Subsequently, the hypervariable region of the spike (S1) gene of positive samples was amplified and sequenced. The IBV nucleic acid was detected in 42% (42/100) of the pooled samples. Phylogenetic analysis of the S1 gene sequences showed that the IBV detected in this study belonged to two distinct genotypes: GI-14, and GI-23, with three clustering within the GI-23 group. One isolate showed a notable relationship with a previously reported Nigerian isolate, sharing 85% nucleotide identity, and formed a cluster within the GI-14 group. Overall, this study established IBV spread and therefore we recommend continuous surveillance to identify the current circulating strain for possible local vaccine development for effective control measures to mitigate the spread of the virus in the study area and the country at large. |
| format | Article |
| id | doaj-art-74b52ca0767e49ae9d9564fcc7f55433 |
| institution | Kabale University |
| issn | 0794-7976 2705-327X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nigerian Academy of Science |
| record_format | Article |
| series | The Proceedings of the Nigerian Academy of Science |
| spelling | doaj-art-74b52ca0767e49ae9d9564fcc7f554332025-08-20T03:35:27ZengNigerian Academy of ScienceThe Proceedings of the Nigerian Academy of Science0794-79762705-327X2025-06-011815363https://doi.org/10.57046/JPIN2612Molecular detection and characterization of infectious bronchitis virus in live bird markets in Jos, Plateau State, NigeriaBitrus Inuwa0Hassan I. Musa1Hanbali I. Umar2Abdulyekin O. Tijani3Shuaibu G. Adamu4Muhammed A. Sodiq5 Ismaila Shittu6Clement Meseko7Tony Joannis8National Veterinary Research Institute, Vom, Plateau State, Nigeria & University of Maiduguri, Borno State, NigeriaUniversity of Maiduguri, Borno State, Nigeria & Universiti Putra Malaysia, 4300 UPM Serdang, Selangor, MalaysiaUniversity of Maiduguri, Borno State, Nigeria University of Maiduguri, Borno State, NigeriaUnivesrity of Maiduguri, Borno State, Nigeria.University of Maiduguri, Borno State, NigeriaNational Veterinary Research Institute, Vom, Plateau State, NigeriaNational Veterinary Research Institute, Vom, Plateau State, NigeriaNational Veterinary Research Institute, Vom, Plateau State, NigeriaInfectious Bronchitis Virus (IBV) is a highly contagious coronavirus of chickens, ducks, turkeys and Pheasants. It mostly affects the respiratory, urinary and reproductive tracts, leading to considerable economic losses in the poultry industry as a result of drop in egg production, undesirable egg quality and poor weight gain. Thus, this study was designed to detect and characterize IBV circulating in live bird markets (LBMs) in Plateau State, Nigeria. One hundred pools of tracheal and cloacal swabs each were collected from two LBMs in Jos. Viral RNA was extracted and screened for IBV using real-time RT-PCR. Subsequently, the hypervariable region of the spike (S1) gene of positive samples was amplified and sequenced. The IBV nucleic acid was detected in 42% (42/100) of the pooled samples. Phylogenetic analysis of the S1 gene sequences showed that the IBV detected in this study belonged to two distinct genotypes: GI-14, and GI-23, with three clustering within the GI-23 group. One isolate showed a notable relationship with a previously reported Nigerian isolate, sharing 85% nucleotide identity, and formed a cluster within the GI-14 group. Overall, this study established IBV spread and therefore we recommend continuous surveillance to identify the current circulating strain for possible local vaccine development for effective control measures to mitigate the spread of the virus in the study area and the country at large.https://nasjournal.org.ng/site/index.php/pnas/article/view/694/371avian coronavirusinfectious bronchitislive bird marketsmolecular characterizationplateau statenigeria |
| spellingShingle | Bitrus Inuwa Hassan I. Musa Hanbali I. Umar Abdulyekin O. Tijani Shuaibu G. Adamu Muhammed A. Sodiq Ismaila Shittu Clement Meseko Tony Joannis Molecular detection and characterization of infectious bronchitis virus in live bird markets in Jos, Plateau State, Nigeria The Proceedings of the Nigerian Academy of Science avian coronavirus infectious bronchitis live bird markets molecular characterization plateau state nigeria |
| title | Molecular detection and characterization of infectious bronchitis virus in live bird markets in Jos, Plateau State, Nigeria |
| title_full | Molecular detection and characterization of infectious bronchitis virus in live bird markets in Jos, Plateau State, Nigeria |
| title_fullStr | Molecular detection and characterization of infectious bronchitis virus in live bird markets in Jos, Plateau State, Nigeria |
| title_full_unstemmed | Molecular detection and characterization of infectious bronchitis virus in live bird markets in Jos, Plateau State, Nigeria |
| title_short | Molecular detection and characterization of infectious bronchitis virus in live bird markets in Jos, Plateau State, Nigeria |
| title_sort | molecular detection and characterization of infectious bronchitis virus in live bird markets in jos plateau state nigeria |
| topic | avian coronavirus infectious bronchitis live bird markets molecular characterization plateau state nigeria |
| url | https://nasjournal.org.ng/site/index.php/pnas/article/view/694/371 |
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