Protocol for assembling photostable fluorescence-labeled tiny DNA origami blocks to observe kinesin cooperation

Summary: Fluorescence-labeled tiny DNA origami blocks (FTOBs) enable observation of kinesin motility and their cooperation with minimal blinking and photobleaching. Here, we present a protocol for assembling FTOBs to observe kinesin cooperation. We describe steps for linking FTOBs and kinesins via a...

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Bibliographic Details
Main Authors: Tomoki Kita, Ryota Sugie, Yuki Suzuki, Shinsuke Niwa
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003727
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