An In Vitro Diacetylcurcumin Study for Periodontitis: A New Approach to Controlling Subgingival Biofilms

<b>Background:</b> Periodontal disease (PD) is a chronic inflammatory condition associated with dysbiotic biofilm, leading to the destruction of bone and periodontal ligament. Scaling and root planing (SRP) is the gold-standard treatment for PD, but some patients may not respond adequate...

Full description

Saved in:
Bibliographic Details
Main Authors: Valdo Antonio Aires da Silva, Bruno Bueno-Silva, Luciene Cristina Figueiredo, Tatiane Tiemi Macedo, Lucas Daylor Aguiar da Silva, Helio Chagas Chaves de Oliveira Junior, Carlos Roberto Polaquini, Luís Octávio Regasini, Janaina de Cássia Orlandi Sardi
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Future Pharmacology
Subjects:
Online Access:https://www.mdpi.com/2673-9879/5/2/19
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849472031298420736
author Valdo Antonio Aires da Silva
Bruno Bueno-Silva
Luciene Cristina Figueiredo
Tatiane Tiemi Macedo
Lucas Daylor Aguiar da Silva
Helio Chagas Chaves de Oliveira Junior
Carlos Roberto Polaquini
Luís Octávio Regasini
Janaina de Cássia Orlandi Sardi
author_facet Valdo Antonio Aires da Silva
Bruno Bueno-Silva
Luciene Cristina Figueiredo
Tatiane Tiemi Macedo
Lucas Daylor Aguiar da Silva
Helio Chagas Chaves de Oliveira Junior
Carlos Roberto Polaquini
Luís Octávio Regasini
Janaina de Cássia Orlandi Sardi
author_sort Valdo Antonio Aires da Silva
collection DOAJ
description <b>Background:</b> Periodontal disease (PD) is a chronic inflammatory condition associated with dysbiotic biofilm, leading to the destruction of bone and periodontal ligament. Scaling and root planing (SRP) is the gold-standard treatment for PD, but some patients may not respond adequately, necessitating adjunctive therapies. This study investigated the antimicrobial activity of diacetylcurcumin (DAC), a modified curcumin, against multispecies subgingival biofilm associated with periodontitis. <b>Methods:</b> The biofilm, containing 40 bacterial species, was cultured for seven days in the Calgary apparatus. Treatments with DAC (200 μg/mL), 0.12% chlorhexidine (CHX), and a vehicle (control) were applied twice daily for 1 min, starting on the third day. On the seventh day, biofilms were analyzed for metabolic activity (MA) and bacterial counts via DNA-DNA hybridization. DAC toxicity was tested on <i>Galleria mellonella</i> larvae. <b>Results:</b> DAC reduced biofilm metabolic activity by 51%, while CHX achieved 88% reduction compared to the vehicle (<i>p</i> < 0.05). DAC also significantly decreased counts of key periodontal pathogens, including <i>P. gingivalis</i>, <i>T. forsythia</i>, <i>P. intermedia</i>, and <i>A. actinomycetemcomitans</i> (<i>p</i> < 0.05). At the tested concentration, DAC showed no toxicity in larvae. <b>Conclusions:</b> These findings suggest that DAC effectively reduces biofilm activity and periodontal pathogen counts, presenting a promising adjunctive therapy for PD.
format Article
id doaj-art-74afad7b542d4608b469a005c85dbef4
institution Kabale University
issn 2673-9879
language English
publishDate 2025-04-01
publisher MDPI AG
record_format Article
series Future Pharmacology
spelling doaj-art-74afad7b542d4608b469a005c85dbef42025-08-20T03:24:38ZengMDPI AGFuture Pharmacology2673-98792025-04-01521910.3390/futurepharmacol5020019An In Vitro Diacetylcurcumin Study for Periodontitis: A New Approach to Controlling Subgingival BiofilmsValdo Antonio Aires da Silva0Bruno Bueno-Silva1Luciene Cristina Figueiredo2Tatiane Tiemi Macedo3Lucas Daylor Aguiar da Silva4Helio Chagas Chaves de Oliveira Junior5Carlos Roberto Polaquini6Luís Octávio Regasini7Janaina de Cássia Orlandi Sardi8Dental Research Division, Guarulhos University, Guarulhos 07023-070, SP, BrazilDental Research Division, Guarulhos University, Guarulhos 07023-070, SP, BrazilDental Research Division, Guarulhos University, Guarulhos 07023-070, SP, BrazilDental Research Division, Guarulhos University, Guarulhos 07023-070, SP, BrazilDental Research Division, Guarulhos University, Guarulhos 07023-070, SP, BrazilDental Research Division, Guarulhos University, Guarulhos 07023-070, SP, BrazilDepartment of Chemistry and Environmental Sciences, Júlio de Mesquita Filho University, São Jose do Rio Preto 15054-000, SP, BrazilDepartment of Chemistry and Environmental Sciences, Júlio de Mesquita Filho University, São Jose do Rio Preto 15054-000, SP, BrazilDental Research Division, Guarulhos University, Guarulhos 07023-070, SP, Brazil<b>Background:</b> Periodontal disease (PD) is a chronic inflammatory condition associated with dysbiotic biofilm, leading to the destruction of bone and periodontal ligament. Scaling and root planing (SRP) is the gold-standard treatment for PD, but some patients may not respond adequately, necessitating adjunctive therapies. This study investigated the antimicrobial activity of diacetylcurcumin (DAC), a modified curcumin, against multispecies subgingival biofilm associated with periodontitis. <b>Methods:</b> The biofilm, containing 40 bacterial species, was cultured for seven days in the Calgary apparatus. Treatments with DAC (200 μg/mL), 0.12% chlorhexidine (CHX), and a vehicle (control) were applied twice daily for 1 min, starting on the third day. On the seventh day, biofilms were analyzed for metabolic activity (MA) and bacterial counts via DNA-DNA hybridization. DAC toxicity was tested on <i>Galleria mellonella</i> larvae. <b>Results:</b> DAC reduced biofilm metabolic activity by 51%, while CHX achieved 88% reduction compared to the vehicle (<i>p</i> < 0.05). DAC also significantly decreased counts of key periodontal pathogens, including <i>P. gingivalis</i>, <i>T. forsythia</i>, <i>P. intermedia</i>, and <i>A. actinomycetemcomitans</i> (<i>p</i> < 0.05). At the tested concentration, DAC showed no toxicity in larvae. <b>Conclusions:</b> These findings suggest that DAC effectively reduces biofilm activity and periodontal pathogen counts, presenting a promising adjunctive therapy for PD.https://www.mdpi.com/2673-9879/5/2/19DACbiofilmperiodontitistoxicity<i>Galleria mellonella</i>
spellingShingle Valdo Antonio Aires da Silva
Bruno Bueno-Silva
Luciene Cristina Figueiredo
Tatiane Tiemi Macedo
Lucas Daylor Aguiar da Silva
Helio Chagas Chaves de Oliveira Junior
Carlos Roberto Polaquini
Luís Octávio Regasini
Janaina de Cássia Orlandi Sardi
An In Vitro Diacetylcurcumin Study for Periodontitis: A New Approach to Controlling Subgingival Biofilms
Future Pharmacology
DAC
biofilm
periodontitis
toxicity
<i>Galleria mellonella</i>
title An In Vitro Diacetylcurcumin Study for Periodontitis: A New Approach to Controlling Subgingival Biofilms
title_full An In Vitro Diacetylcurcumin Study for Periodontitis: A New Approach to Controlling Subgingival Biofilms
title_fullStr An In Vitro Diacetylcurcumin Study for Periodontitis: A New Approach to Controlling Subgingival Biofilms
title_full_unstemmed An In Vitro Diacetylcurcumin Study for Periodontitis: A New Approach to Controlling Subgingival Biofilms
title_short An In Vitro Diacetylcurcumin Study for Periodontitis: A New Approach to Controlling Subgingival Biofilms
title_sort in vitro diacetylcurcumin study for periodontitis a new approach to controlling subgingival biofilms
topic DAC
biofilm
periodontitis
toxicity
<i>Galleria mellonella</i>
url https://www.mdpi.com/2673-9879/5/2/19
work_keys_str_mv AT valdoantonioairesdasilva aninvitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT brunobuenosilva aninvitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT lucienecristinafigueiredo aninvitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT tatianetiemimacedo aninvitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT lucasdayloraguiardasilva aninvitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT heliochagaschavesdeoliveirajunior aninvitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT carlosrobertopolaquini aninvitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT luisoctavioregasini aninvitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT janainadecassiaorlandisardi aninvitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT valdoantonioairesdasilva invitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT brunobuenosilva invitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT lucienecristinafigueiredo invitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT tatianetiemimacedo invitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT lucasdayloraguiardasilva invitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT heliochagaschavesdeoliveirajunior invitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT carlosrobertopolaquini invitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT luisoctavioregasini invitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms
AT janainadecassiaorlandisardi invitrodiacetylcurcuminstudyforperiodontitisanewapproachtocontrollingsubgingivalbiofilms