Fluorescent–Electrochemical–Colorimetric Triple-Model Immunoassays with Multifunctional Metal–Organic Frameworks for Signal Amplification
Multimode immunoassays based on multiple response mechanisms have received great attention due to their capacity to effectively improve the accuracy and reliability of biosensing platforms. However, few strategies have been reported for triple-mode immunoassays due to the shortage of multifunctional...
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MDPI AG
2025-06-01
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| author | Ning Xia Chuye Zheng Gang Liu |
| author_facet | Ning Xia Chuye Zheng Gang Liu |
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| description | Multimode immunoassays based on multiple response mechanisms have received great attention due to their capacity to effectively improve the accuracy and reliability of biosensing platforms. However, few strategies have been reported for triple-mode immunoassays due to the shortage of multifunctional sensing materials and the incompatibility of signal transduction methods in different detection modes. In this work, a fluorescent–electrochemical–colorimetric triple-mode immunoassay platform was proposed with Cu-based metal–organic frameworks (MOFs) as the signal labels. The captured Cu-MOFs were successfully decomposed under an acidic condition, leading to the release of numerous Cu<sup>2+</sup> ions and 2-aminobenzene-1,4-dicarboxylic acid (NH<sub>2</sub>-BDC) ligands. The released NH<sub>2</sub>-BDC were determined by fluorescence titration. Meanwhile, the released Cu<sup>2+</sup> were readily quantified by differential pulse voltammetry (DPV) and simply detected through the catalytic oxidation of chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB). Taking alpha-fetoprotein (AFP) as a model analyte, the designed triple-mode immunoassays showed good performances with the linear range of 10–200 pg/mL, 10–200 pg/mL, and 1–100 pg/mL for the fluorescent, electrochemical, and colorimetric modes, respectively. The proposed triple-mode biosensing platforms show great potential for the applications in disease diagnosis, since they can be easily extended to other bioassays by changing the targets and recognition elements. |
| format | Article |
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| language | English |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-74a9a19aee504ceaae2147dfdf6919b92025-08-20T02:24:18ZengMDPI AGBiosensors2079-63742025-06-0115637610.3390/bios15060376Fluorescent–Electrochemical–Colorimetric Triple-Model Immunoassays with Multifunctional Metal–Organic Frameworks for Signal AmplificationNing Xia0Chuye Zheng1Gang Liu2Henan Province Key Laboratory of New Opto-Electronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, ChinaHenan Province Key Laboratory of New Opto-Electronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, ChinaHenan Province Key Laboratory of New Opto-Electronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, ChinaMultimode immunoassays based on multiple response mechanisms have received great attention due to their capacity to effectively improve the accuracy and reliability of biosensing platforms. However, few strategies have been reported for triple-mode immunoassays due to the shortage of multifunctional sensing materials and the incompatibility of signal transduction methods in different detection modes. In this work, a fluorescent–electrochemical–colorimetric triple-mode immunoassay platform was proposed with Cu-based metal–organic frameworks (MOFs) as the signal labels. The captured Cu-MOFs were successfully decomposed under an acidic condition, leading to the release of numerous Cu<sup>2+</sup> ions and 2-aminobenzene-1,4-dicarboxylic acid (NH<sub>2</sub>-BDC) ligands. The released NH<sub>2</sub>-BDC were determined by fluorescence titration. Meanwhile, the released Cu<sup>2+</sup> were readily quantified by differential pulse voltammetry (DPV) and simply detected through the catalytic oxidation of chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB). Taking alpha-fetoprotein (AFP) as a model analyte, the designed triple-mode immunoassays showed good performances with the linear range of 10–200 pg/mL, 10–200 pg/mL, and 1–100 pg/mL for the fluorescent, electrochemical, and colorimetric modes, respectively. The proposed triple-mode biosensing platforms show great potential for the applications in disease diagnosis, since they can be easily extended to other bioassays by changing the targets and recognition elements.https://www.mdpi.com/2079-6374/15/6/376triple-model immunoassaysmetal–organic frameworksfluorescence immunoassayelectrochemical immunoassaycolorimetric immunoassay |
| spellingShingle | Ning Xia Chuye Zheng Gang Liu Fluorescent–Electrochemical–Colorimetric Triple-Model Immunoassays with Multifunctional Metal–Organic Frameworks for Signal Amplification Biosensors triple-model immunoassays metal–organic frameworks fluorescence immunoassay electrochemical immunoassay colorimetric immunoassay |
| title | Fluorescent–Electrochemical–Colorimetric Triple-Model Immunoassays with Multifunctional Metal–Organic Frameworks for Signal Amplification |
| title_full | Fluorescent–Electrochemical–Colorimetric Triple-Model Immunoassays with Multifunctional Metal–Organic Frameworks for Signal Amplification |
| title_fullStr | Fluorescent–Electrochemical–Colorimetric Triple-Model Immunoassays with Multifunctional Metal–Organic Frameworks for Signal Amplification |
| title_full_unstemmed | Fluorescent–Electrochemical–Colorimetric Triple-Model Immunoassays with Multifunctional Metal–Organic Frameworks for Signal Amplification |
| title_short | Fluorescent–Electrochemical–Colorimetric Triple-Model Immunoassays with Multifunctional Metal–Organic Frameworks for Signal Amplification |
| title_sort | fluorescent electrochemical colorimetric triple model immunoassays with multifunctional metal organic frameworks for signal amplification |
| topic | triple-model immunoassays metal–organic frameworks fluorescence immunoassay electrochemical immunoassay colorimetric immunoassay |
| url | https://www.mdpi.com/2079-6374/15/6/376 |
| work_keys_str_mv | AT ningxia fluorescentelectrochemicalcolorimetrictriplemodelimmunoassayswithmultifunctionalmetalorganicframeworksforsignalamplification AT chuyezheng fluorescentelectrochemicalcolorimetrictriplemodelimmunoassayswithmultifunctionalmetalorganicframeworksforsignalamplification AT gangliu fluorescentelectrochemicalcolorimetrictriplemodelimmunoassayswithmultifunctionalmetalorganicframeworksforsignalamplification |