Induction conditions that promote the effect of glycerol on recombinant protein production in Escherichia coli

Induction conditions that promote the effect of glycerol on recombinant protein production in Escherichia coli

Proinsulin was expressed by Escherichia coli SHuffle T7 with pET system in minimal medium containing glucose (Glu medium), glucose and glycerol (GluGly medium) or glycerol (Gly medium). With 100 μM IPTG, proinsulin production did not increase with glycerol. In contrast, proinsulin production per med...

Full description

Saved in:
Bibliographic Details
Main Authors: Yoshihiro Ojima, Hajime Saito, Shintaro Miyuki, Koichi Fukunaga, Terumichi Tsuboi, Masayuki Azuma
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Biotechnology Reports
Subjects:
Glycerol
E. coli
Proinsulin production
Catabolite repression
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X25000256
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items

Similar Items