TPP crosslinking influence on the effect of sprayed dried microparticles based on industry waste sourced-chitosan over crop pathogens

The use of fishing industry waste residues for biotechnological developments is blossoming in the last decade to improve the management of marine resources while reducing the environmental impact of the fisheries sector. Chitosan (CS), is a natural and nontoxic biopolymer that exerts great antimicro...

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Main Authors: Florencia Anabel Mesas, Merari Tumin Chevalier, Julieta Renée Mendieta, María Cecilia Terrile, Pablo Villanueva, Claudia Anahi Casalongué, Vera Alejandra Álvarez, Alberto Antonio Chevalier, Sergio Martin-Saldaña
Format: Article
Language:English
Published: Elsevier 2024-01-01
Series:Next Sustainability
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Online Access:http://www.sciencedirect.com/science/article/pii/S2949823624000242
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author Florencia Anabel Mesas
Merari Tumin Chevalier
Julieta Renée Mendieta
María Cecilia Terrile
Pablo Villanueva
Claudia Anahi Casalongué
Vera Alejandra Álvarez
Alberto Antonio Chevalier
Sergio Martin-Saldaña
author_facet Florencia Anabel Mesas
Merari Tumin Chevalier
Julieta Renée Mendieta
María Cecilia Terrile
Pablo Villanueva
Claudia Anahi Casalongué
Vera Alejandra Álvarez
Alberto Antonio Chevalier
Sergio Martin-Saldaña
author_sort Florencia Anabel Mesas
collection DOAJ
description The use of fishing industry waste residues for biotechnological developments is blossoming in the last decade to improve the management of marine resources while reducing the environmental impact of the fisheries sector. Chitosan (CS), is a natural and nontoxic biopolymer that exerts great antimicrobial properties and is being considered as GRAS by the United States Food and Drug Administration. In this study, we aimed to produce at a gram scale high molecular weight CS microparticles (CS-MP) from a low quality commercial raw material intended to develop an environmental friendly antimicrobial. Thus, we aimed to test the effect of sodium tripolyphosphate (TPP) used as a crosslinker on the antimicrobial performance of the CS-MP. Hence, we synthesized CS using shrimp fishing industry waste from Argentinean Patagonia at a pilot scale in order to be able to provide a cost-effective second life to the industry waste. By varying the ratio polymer/crosslinker, we studied its influence on the final physicochemical properties as well as their performance against representative crop pathogens: Pseudomonas syringae pv. tomato DC 3000 and Fusarium solani f. sp. eumartii. Our results showed a correlation between the surface charge exhibited by CS-MPs and their antimicrobial properties, through an imbalance on fungal membrane permeability. Thus, given the difference in the CS-MPs performance, it is clear that the electrostatic interaction of the particles with the negatively charged surface of the microorganism plays a key role in determining their ultimate activity. The CS-MPs exhibited great potential against crop pathogens providing a second life to fishing industry waste by developing an eco-friendly plant protectant from a valuable synergy of the national industry and academics.
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spelling doaj-art-74a0ebf19d924865a5e61ba1b720b8952025-08-20T02:38:38ZengElsevierNext Sustainability2949-82362024-01-01410004710.1016/j.nxsust.2024.100047TPP crosslinking influence on the effect of sprayed dried microparticles based on industry waste sourced-chitosan over crop pathogensFlorencia Anabel Mesas0Merari Tumin Chevalier1Julieta Renée Mendieta2María Cecilia Terrile3Pablo Villanueva4Claudia Anahi Casalongué5Vera Alejandra Álvarez6Alberto Antonio Chevalier7Sergio Martin-Saldaña8Universidad Nacional de Mar del Plata, CONICET, Instituto de Investigaciones Biológicas IIB, Mar Del Plata, ArgentinaGihon Laboratorios Químicos SRL, Mar Del Plata, Argentina; Universidad Nacional de Mar del Plata, CONICET, Instituto de Investigación de Ciencia & Tecnología de Materiales INTEMA, Grupo Materiales Compuestos Termoplásticos, Mar Del Plata, ArgentinaUniversidad Nacional de Mar del Plata, CONICET, Instituto de Investigaciones Biológicas IIB, Mar Del Plata, ArgentinaUniversidad Nacional de Mar del Plata, CONICET, Instituto de Investigaciones Biológicas IIB, Mar Del Plata, ArgentinaGihon Laboratorios Químicos SRL, Mar Del Plata, ArgentinaUniversidad Nacional de Mar del Plata, Mar Del Plata, ArgentinaUniversidad Nacional de Mar del Plata, CONICET, Instituto de Investigación de Ciencia & Tecnología de Materiales INTEMA, Grupo Materiales Compuestos Termoplásticos, Mar Del Plata, ArgentinaGihon Laboratorios Químicos SRL, Mar Del Plata, ArgentinaGihon Laboratorios Químicos SRL, Mar Del Plata, Argentina; Corresponding author.The use of fishing industry waste residues for biotechnological developments is blossoming in the last decade to improve the management of marine resources while reducing the environmental impact of the fisheries sector. Chitosan (CS), is a natural and nontoxic biopolymer that exerts great antimicrobial properties and is being considered as GRAS by the United States Food and Drug Administration. In this study, we aimed to produce at a gram scale high molecular weight CS microparticles (CS-MP) from a low quality commercial raw material intended to develop an environmental friendly antimicrobial. Thus, we aimed to test the effect of sodium tripolyphosphate (TPP) used as a crosslinker on the antimicrobial performance of the CS-MP. Hence, we synthesized CS using shrimp fishing industry waste from Argentinean Patagonia at a pilot scale in order to be able to provide a cost-effective second life to the industry waste. By varying the ratio polymer/crosslinker, we studied its influence on the final physicochemical properties as well as their performance against representative crop pathogens: Pseudomonas syringae pv. tomato DC 3000 and Fusarium solani f. sp. eumartii. Our results showed a correlation between the surface charge exhibited by CS-MPs and their antimicrobial properties, through an imbalance on fungal membrane permeability. Thus, given the difference in the CS-MPs performance, it is clear that the electrostatic interaction of the particles with the negatively charged surface of the microorganism plays a key role in determining their ultimate activity. The CS-MPs exhibited great potential against crop pathogens providing a second life to fishing industry waste by developing an eco-friendly plant protectant from a valuable synergy of the national industry and academics.http://www.sciencedirect.com/science/article/pii/S2949823624000242ChitosanMicroparticlesCrosslinkingAntimicrobialCrop pathogen
spellingShingle Florencia Anabel Mesas
Merari Tumin Chevalier
Julieta Renée Mendieta
María Cecilia Terrile
Pablo Villanueva
Claudia Anahi Casalongué
Vera Alejandra Álvarez
Alberto Antonio Chevalier
Sergio Martin-Saldaña
TPP crosslinking influence on the effect of sprayed dried microparticles based on industry waste sourced-chitosan over crop pathogens
Next Sustainability
Chitosan
Microparticles
Crosslinking
Antimicrobial
Crop pathogen
title TPP crosslinking influence on the effect of sprayed dried microparticles based on industry waste sourced-chitosan over crop pathogens
title_full TPP crosslinking influence on the effect of sprayed dried microparticles based on industry waste sourced-chitosan over crop pathogens
title_fullStr TPP crosslinking influence on the effect of sprayed dried microparticles based on industry waste sourced-chitosan over crop pathogens
title_full_unstemmed TPP crosslinking influence on the effect of sprayed dried microparticles based on industry waste sourced-chitosan over crop pathogens
title_short TPP crosslinking influence on the effect of sprayed dried microparticles based on industry waste sourced-chitosan over crop pathogens
title_sort tpp crosslinking influence on the effect of sprayed dried microparticles based on industry waste sourced chitosan over crop pathogens
topic Chitosan
Microparticles
Crosslinking
Antimicrobial
Crop pathogen
url http://www.sciencedirect.com/science/article/pii/S2949823624000242
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