Culture and Characterization of Differentiated Airway Organoids from Fetal Mouse Lung Proximal Progenitors
Developing a physiologically relevant in vitro model of the respiratory epithelium is critical for understanding lung development and respiratory diseases. Here, we describe a detailed protocol in which the fetal mouse proximal epithelial progenitors were differentiated into 3D airway organoids, whi...
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| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2024-12-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5129&type=0 |
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| author | Zhonghui Zhang Chengxu Tao Qiuling Li |
| author_facet | Zhonghui Zhang Chengxu Tao Qiuling Li |
| author_sort | Zhonghui Zhang |
| collection | DOAJ |
| description | Developing a physiologically relevant in vitro model of the respiratory epithelium is critical for understanding lung development and respiratory diseases. Here, we describe a detailed protocol in which the fetal mouse proximal epithelial progenitors were differentiated into 3D airway organoids, which contain terminal-differentiated ciliated cells and basal stem cells. These differentiated airway organoids could constitute an excellent experimental model to elucidate the molecular mechanisms of airway development and epithelial cell fate determination and offer an important tool for establishing pulmonary dysplasia disease in vitro. |
| format | Article |
| id | doaj-art-749da89bbebd4512a07431747bca3ef7 |
| institution | OA Journals |
| issn | 2331-8325 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-749da89bbebd4512a07431747bca3ef72025-08-20T02:38:59ZengBio-protocol LLCBio-Protocol2331-83252024-12-01142310.21769/BioProtoc.5129Culture and Characterization of Differentiated Airway Organoids from Fetal Mouse Lung Proximal ProgenitorsZhonghui Zhang0Chengxu Tao1Qiuling Li2From the Institute of Health Sciences and Technology, Institutes of Physical Science and Information Technology, Anhui University, Hefei, ChinaFrom the Institute of Health Sciences and Technology, Institutes of Physical Science and Information Technology, Anhui University, Hefei, ChinaFrom the Institute of Health Sciences and Technology, Institutes of Physical Science and Information Technology, Anhui University, Hefei, ChinaDeveloping a physiologically relevant in vitro model of the respiratory epithelium is critical for understanding lung development and respiratory diseases. Here, we describe a detailed protocol in which the fetal mouse proximal epithelial progenitors were differentiated into 3D airway organoids, which contain terminal-differentiated ciliated cells and basal stem cells. These differentiated airway organoids could constitute an excellent experimental model to elucidate the molecular mechanisms of airway development and epithelial cell fate determination and offer an important tool for establishing pulmonary dysplasia disease in vitro.https://bio-protocol.org/en/bpdetail?id=5129&type=0 |
| spellingShingle | Zhonghui Zhang Chengxu Tao Qiuling Li Culture and Characterization of Differentiated Airway Organoids from Fetal Mouse Lung Proximal Progenitors Bio-Protocol |
| title | Culture and Characterization of Differentiated Airway Organoids from Fetal Mouse Lung Proximal Progenitors |
| title_full | Culture and Characterization of Differentiated Airway Organoids from Fetal Mouse Lung Proximal Progenitors |
| title_fullStr | Culture and Characterization of Differentiated Airway Organoids from Fetal Mouse Lung Proximal Progenitors |
| title_full_unstemmed | Culture and Characterization of Differentiated Airway Organoids from Fetal Mouse Lung Proximal Progenitors |
| title_short | Culture and Characterization of Differentiated Airway Organoids from Fetal Mouse Lung Proximal Progenitors |
| title_sort | culture and characterization of differentiated airway organoids from fetal mouse lung proximal progenitors |
| url | https://bio-protocol.org/en/bpdetail?id=5129&type=0 |
| work_keys_str_mv | AT zhonghuizhang cultureandcharacterizationofdifferentiatedairwayorganoidsfromfetalmouselungproximalprogenitors AT chengxutao cultureandcharacterizationofdifferentiatedairwayorganoidsfromfetalmouselungproximalprogenitors AT qiulingli cultureandcharacterizationofdifferentiatedairwayorganoidsfromfetalmouselungproximalprogenitors |