Development and Characterization of Anti-Nitr9 Antibodies
The novel immune-type receptors (NITRs), which have been described in numerous bony fish species, are encoded by multigene families of inhibitory and activating receptors and are predicted to be functional orthologs to the mammalian natural killer cell receptors (NKRs). Within the zebrafish NITR fam...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Advances in Hematology |
| Online Access: | http://dx.doi.org/10.1155/2012/596925 |
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| author | Radhika N. Shah Ivan Rodriguez-Nunez Donna D. Eason Robert N. Haire Julien Y. Bertrand Valērie Wittamer David Traver Shila K. Nordone Gary W. Litman Jeffrey A. Yoder |
| author_facet | Radhika N. Shah Ivan Rodriguez-Nunez Donna D. Eason Robert N. Haire Julien Y. Bertrand Valērie Wittamer David Traver Shila K. Nordone Gary W. Litman Jeffrey A. Yoder |
| author_sort | Radhika N. Shah |
| collection | DOAJ |
| description | The novel immune-type receptors (NITRs), which have been described in numerous bony fish species, are encoded by multigene families of inhibitory and activating receptors and are predicted to be functional orthologs to the mammalian natural killer cell receptors (NKRs). Within the zebrafish NITR family, nitr9 is the only gene predicted to encode an activating receptor. However, alternative RNA splicing generates three distinct nitr9 transcripts, each of which encodes a different isoform. Although nitr9 transcripts have been detected in zebrafish lymphocytes, the specific hematopoietic lineage(s) that expresses Nitr9 remains to be determined. In an effort to better understand the role of NITRs in zebrafish immunity, anti-Nitr9 monoclonal antibodies were generated and evaluated for the ability to recognize the three Nitr9 isoforms. The application of these antibodies to flow cytometry should prove to be useful for identifying the specific lymphocyte lineages that express Nitr9 and may permit the isolation of Nitr9-expressing cells that can be directly assessed for cytotoxic (e.g., NK) function. |
| format | Article |
| id | doaj-art-74913a2dd9224e9685501ae84ad62929 |
| institution | Kabale University |
| issn | 1687-9104 1687-9112 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Hematology |
| spelling | doaj-art-74913a2dd9224e9685501ae84ad629292025-02-03T05:44:36ZengWileyAdvances in Hematology1687-91041687-91122012-01-01201210.1155/2012/596925596925Development and Characterization of Anti-Nitr9 AntibodiesRadhika N. Shah0Ivan Rodriguez-Nunez1Donna D. Eason2Robert N. Haire3Julien Y. Bertrand4Valērie Wittamer5David Traver6Shila K. Nordone7Gary W. Litman8Jeffrey A. Yoder9Department of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USADepartment of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USAChildren’s Research Institute, Department of Pediatrics, University of South Florida College of Medicine, 140 Seventh Avenue South, St. Petersburg, FL 33701, USAChildren’s Research Institute, Department of Pediatrics, University of South Florida College of Medicine, 140 Seventh Avenue South, St. Petersburg, FL 33701, USADepartment of Pathology and Immunology, University of Geneva School of Medicine, Rue Michel-Servet 1, 1211 Geneva 4, SwitzerlandDepartment of Cellular and Molecular Medicine and Section of Cell and Developmental Biology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0380, USADepartment of Cellular and Molecular Medicine and Section of Cell and Developmental Biology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0380, USADepartment of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USAChildren’s Research Institute, Department of Pediatrics, University of South Florida College of Medicine, 140 Seventh Avenue South, St. Petersburg, FL 33701, USADepartment of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USAThe novel immune-type receptors (NITRs), which have been described in numerous bony fish species, are encoded by multigene families of inhibitory and activating receptors and are predicted to be functional orthologs to the mammalian natural killer cell receptors (NKRs). Within the zebrafish NITR family, nitr9 is the only gene predicted to encode an activating receptor. However, alternative RNA splicing generates three distinct nitr9 transcripts, each of which encodes a different isoform. Although nitr9 transcripts have been detected in zebrafish lymphocytes, the specific hematopoietic lineage(s) that expresses Nitr9 remains to be determined. In an effort to better understand the role of NITRs in zebrafish immunity, anti-Nitr9 monoclonal antibodies were generated and evaluated for the ability to recognize the three Nitr9 isoforms. The application of these antibodies to flow cytometry should prove to be useful for identifying the specific lymphocyte lineages that express Nitr9 and may permit the isolation of Nitr9-expressing cells that can be directly assessed for cytotoxic (e.g., NK) function.http://dx.doi.org/10.1155/2012/596925 |
| spellingShingle | Radhika N. Shah Ivan Rodriguez-Nunez Donna D. Eason Robert N. Haire Julien Y. Bertrand Valērie Wittamer David Traver Shila K. Nordone Gary W. Litman Jeffrey A. Yoder Development and Characterization of Anti-Nitr9 Antibodies Advances in Hematology |
| title | Development and Characterization of Anti-Nitr9 Antibodies |
| title_full | Development and Characterization of Anti-Nitr9 Antibodies |
| title_fullStr | Development and Characterization of Anti-Nitr9 Antibodies |
| title_full_unstemmed | Development and Characterization of Anti-Nitr9 Antibodies |
| title_short | Development and Characterization of Anti-Nitr9 Antibodies |
| title_sort | development and characterization of anti nitr9 antibodies |
| url | http://dx.doi.org/10.1155/2012/596925 |
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