Composition analysis of acid hydrolysates from Cucurbita moschata Duch. polysaccharides and their effect on oxidative stress resistance of Caenorhabditis elegans
Pumpkin polysaccharides (PPe) have a variety of bioactive effects and our previous research showed the acid hydrolysate (PPe-S, a mixture) from PPe had an antioxidative capacity both in vitro and in viro. The aim of this study was to purify PPe-S and investigate the antioxidant stress effects of 2 p...
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Tsinghua University Press
2023-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002014 |
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| author | Wen Jiang Yakun Zhang Jiangying Zeng Jing Yao Aoxue Lu Zhiyu Fang Ge Wang Weimin Wang Yongjun Zhang |
| author_facet | Wen Jiang Yakun Zhang Jiangying Zeng Jing Yao Aoxue Lu Zhiyu Fang Ge Wang Weimin Wang Yongjun Zhang |
| author_sort | Wen Jiang |
| collection | DOAJ |
| description | Pumpkin polysaccharides (PPe) have a variety of bioactive effects and our previous research showed the acid hydrolysate (PPe-S, a mixture) from PPe had an antioxidative capacity both in vitro and in viro. The aim of this study was to purify PPe-S and investigate the antioxidant stress effects of 2 purified components (PPe-S-1 and PPe-S-2) using Caenorhabditis elegans as model organism. The results showed that PPe-S-2 had a notable antioxidant effect, and could significantly enhance the activities of antioxidant enzymes including superoxide dismutase (SOD) (P < 0.01), catalase (CAT) (P < 0.01) and glutathione reductase (GR) (P < 0.05), and increase the level of glutathione (GSH) (P < 0.01), and decreased the content of malondialdehyde (MDA) (P < 0.05). PPe-S-2 could significantly extend the survival time of C. elegans (P < 0.01), which were stress-induced by hydrogen peroxide and methyl viologen. PPe-S-2 was a heteropolys accharide composed of glucose, arabinose, rhamnose and galactose with the molar ratio of 1.00:0.03:0.02:0.14. The molecular weight of PPe-S-2 was 0.73 kDa detected by high performance liquid chromatography.These studies demonstrated that PPe-S-2 obtained by the acid hydrolysis of PPe had a prominent protective effect to the damage induced by the intracellular free radical generating agents. |
| format | Article |
| id | doaj-art-7482c3b86f8f462c8fe3b2bb25e1eb9d |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-7482c3b86f8f462c8fe3b2bb25e1eb9d2025-02-03T06:53:35ZengTsinghua University PressFood Science and Human Wellness2213-45302023-05-01123795800Composition analysis of acid hydrolysates from Cucurbita moschata Duch. polysaccharides and their effect on oxidative stress resistance of Caenorhabditis elegansWen Jiang0Yakun Zhang1Jiangying Zeng2Jing Yao3Aoxue Lu4Zhiyu Fang5Ge Wang6Weimin Wang7Yongjun Zhang8Key Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaCollege of Modern Science and Technology, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou 310018, China; Corresponding author at: College of Life Sciences, China Jiliang University, Hangzhou 310018, China.Pumpkin polysaccharides (PPe) have a variety of bioactive effects and our previous research showed the acid hydrolysate (PPe-S, a mixture) from PPe had an antioxidative capacity both in vitro and in viro. The aim of this study was to purify PPe-S and investigate the antioxidant stress effects of 2 purified components (PPe-S-1 and PPe-S-2) using Caenorhabditis elegans as model organism. The results showed that PPe-S-2 had a notable antioxidant effect, and could significantly enhance the activities of antioxidant enzymes including superoxide dismutase (SOD) (P < 0.01), catalase (CAT) (P < 0.01) and glutathione reductase (GR) (P < 0.05), and increase the level of glutathione (GSH) (P < 0.01), and decreased the content of malondialdehyde (MDA) (P < 0.05). PPe-S-2 could significantly extend the survival time of C. elegans (P < 0.01), which were stress-induced by hydrogen peroxide and methyl viologen. PPe-S-2 was a heteropolys accharide composed of glucose, arabinose, rhamnose and galactose with the molar ratio of 1.00:0.03:0.02:0.14. The molecular weight of PPe-S-2 was 0.73 kDa detected by high performance liquid chromatography.These studies demonstrated that PPe-S-2 obtained by the acid hydrolysis of PPe had a prominent protective effect to the damage induced by the intracellular free radical generating agents.http://www.sciencedirect.com/science/article/pii/S2213453022002014Pumpkin polysaccharidesAcidhydrolysisCaenorhabditis elegans modelOxidative stress resistance |
| spellingShingle | Wen Jiang Yakun Zhang Jiangying Zeng Jing Yao Aoxue Lu Zhiyu Fang Ge Wang Weimin Wang Yongjun Zhang Composition analysis of acid hydrolysates from Cucurbita moschata Duch. polysaccharides and their effect on oxidative stress resistance of Caenorhabditis elegans Food Science and Human Wellness Pumpkin polysaccharides Acidhydrolysis Caenorhabditis elegans model Oxidative stress resistance |
| title | Composition analysis of acid hydrolysates from Cucurbita moschata Duch. polysaccharides and their effect on oxidative stress resistance of Caenorhabditis elegans |
| title_full | Composition analysis of acid hydrolysates from Cucurbita moschata Duch. polysaccharides and their effect on oxidative stress resistance of Caenorhabditis elegans |
| title_fullStr | Composition analysis of acid hydrolysates from Cucurbita moschata Duch. polysaccharides and their effect on oxidative stress resistance of Caenorhabditis elegans |
| title_full_unstemmed | Composition analysis of acid hydrolysates from Cucurbita moschata Duch. polysaccharides and their effect on oxidative stress resistance of Caenorhabditis elegans |
| title_short | Composition analysis of acid hydrolysates from Cucurbita moschata Duch. polysaccharides and their effect on oxidative stress resistance of Caenorhabditis elegans |
| title_sort | composition analysis of acid hydrolysates from cucurbita moschata duch polysaccharides and their effect on oxidative stress resistance of caenorhabditis elegans |
| topic | Pumpkin polysaccharides Acidhydrolysis Caenorhabditis elegans model Oxidative stress resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002014 |
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