Effect of Ionic Strength on Porphyrin Drugs Interaction with Quadruplex DNA Formed by the Promoter Region of C-myc and Bcl2 Oncogenes
C-myc and Bcl2 are well characterized oncogenes that are capable of forming G-quadruplex structures. Promoter regions of C-myc and Bcl2 forming G-quadruplex structures are chemically synthesized and G-quadruplex structure is formed in presence of 100 mM potassium ion. Three different porphyrin drugs...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
|
| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.4061/2010/146418 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849414244737482752 |
|---|---|
| author | Narayana Nagesh Varun K. Sharma A. Ganesh Kumar Edwin A. Lewis |
| author_facet | Narayana Nagesh Varun K. Sharma A. Ganesh Kumar Edwin A. Lewis |
| author_sort | Narayana Nagesh |
| collection | DOAJ |
| description | C-myc and Bcl2 are well characterized oncogenes that are capable of forming G-quadruplex structures. Promoter regions of C-myc and Bcl2 forming G-quadruplex structures are chemically synthesized and G-quadruplex structure is formed in presence of 100 mM potassium ion. Three different porphyrin drugs, namely TMPyP2, TMPyP3, and TMPyP4 are allowed to interact with quadruplex DNA complex and the site and nature of interaction are studied. Drug interactions with quadruplex DNA were carried out in different potassium ionic strengths using fluorescence spectroscopy. It is found that fluorescence hypochromicity decreases with an increase in ionic strength in the case of TMPyP4, TMPyP3, and TMPyP2. Fluorescence titration studies and Job plots indicate that four molecules of TMPyP4, two molecules of TMPyP3 and TMPyP2 are interacting with one molecule of quadruplex DNA. |
| format | Article |
| id | doaj-art-747fbd49f62049e8b27e299a92799400 |
| institution | Kabale University |
| issn | 2090-021X |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-747fbd49f62049e8b27e299a927994002025-08-20T03:33:53ZengWileyJournal of Nucleic Acids2090-021X2010-01-01201010.4061/2010/146418146418Effect of Ionic Strength on Porphyrin Drugs Interaction with Quadruplex DNA Formed by the Promoter Region of C-myc and Bcl2 OncogenesNarayana Nagesh0Varun K. Sharma1A. Ganesh Kumar2Edwin A. Lewis3Department of Biophysics, Center for Cellular and Molecular Biology, Hyderabad-500 007, IndiaDepartment of Biophysics, Center for Cellular and Molecular Biology, Hyderabad-500 007, IndiaDepartment of Biomedical Engineering, Sathyabama University, Chennai-600 087, IndiaDepartment of Chemistry, Mississippi State University, Mississippi State, MS 39762, USAC-myc and Bcl2 are well characterized oncogenes that are capable of forming G-quadruplex structures. Promoter regions of C-myc and Bcl2 forming G-quadruplex structures are chemically synthesized and G-quadruplex structure is formed in presence of 100 mM potassium ion. Three different porphyrin drugs, namely TMPyP2, TMPyP3, and TMPyP4 are allowed to interact with quadruplex DNA complex and the site and nature of interaction are studied. Drug interactions with quadruplex DNA were carried out in different potassium ionic strengths using fluorescence spectroscopy. It is found that fluorescence hypochromicity decreases with an increase in ionic strength in the case of TMPyP4, TMPyP3, and TMPyP2. Fluorescence titration studies and Job plots indicate that four molecules of TMPyP4, two molecules of TMPyP3 and TMPyP2 are interacting with one molecule of quadruplex DNA.http://dx.doi.org/10.4061/2010/146418 |
| spellingShingle | Narayana Nagesh Varun K. Sharma A. Ganesh Kumar Edwin A. Lewis Effect of Ionic Strength on Porphyrin Drugs Interaction with Quadruplex DNA Formed by the Promoter Region of C-myc and Bcl2 Oncogenes Journal of Nucleic Acids |
| title | Effect of Ionic Strength on Porphyrin Drugs Interaction with Quadruplex DNA Formed by the Promoter Region of C-myc and Bcl2 Oncogenes |
| title_full | Effect of Ionic Strength on Porphyrin Drugs Interaction with Quadruplex DNA Formed by the Promoter Region of C-myc and Bcl2 Oncogenes |
| title_fullStr | Effect of Ionic Strength on Porphyrin Drugs Interaction with Quadruplex DNA Formed by the Promoter Region of C-myc and Bcl2 Oncogenes |
| title_full_unstemmed | Effect of Ionic Strength on Porphyrin Drugs Interaction with Quadruplex DNA Formed by the Promoter Region of C-myc and Bcl2 Oncogenes |
| title_short | Effect of Ionic Strength on Porphyrin Drugs Interaction with Quadruplex DNA Formed by the Promoter Region of C-myc and Bcl2 Oncogenes |
| title_sort | effect of ionic strength on porphyrin drugs interaction with quadruplex dna formed by the promoter region of c myc and bcl2 oncogenes |
| url | http://dx.doi.org/10.4061/2010/146418 |
| work_keys_str_mv | AT narayananagesh effectofionicstrengthonporphyrindrugsinteractionwithquadruplexdnaformedbythepromoterregionofcmycandbcl2oncogenes AT varunksharma effectofionicstrengthonporphyrindrugsinteractionwithquadruplexdnaformedbythepromoterregionofcmycandbcl2oncogenes AT aganeshkumar effectofionicstrengthonporphyrindrugsinteractionwithquadruplexdnaformedbythepromoterregionofcmycandbcl2oncogenes AT edwinalewis effectofionicstrengthonporphyrindrugsinteractionwithquadruplexdnaformedbythepromoterregionofcmycandbcl2oncogenes |