European distribution and intramuscular pathogenicity of divergent lyssaviruses West Caucasian bat virus and Lleida bat lyssavirus
Summary: Among lyssaviruses, West Caucasian bat virus (WCBV) and Lleida bat lyssavirus (LLEBV) raise concern as their divergence from rabies virus leads to the inefficacy of available prophylactic agents. Both viruses were described in the bat Miniopterus schreibersii. We investigated the European d...
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Elsevier
2025-02-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224029651 |
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| author | Stefania Leopardi Laurent Dacheux Jordi Serra-Cobo Ágota Ábrahám Branka Bajić Hervé Bourhy Szilárd-Lehel Bücs Ivana Budinski Martina Castellan Petra Drzewniokova Heliana Dundarova Francesca Festa Lauriane Kergoat Maxime Leuchtmann Marc López-Roig Dominique Pontier Maria Francesca Priore Emmanuelle Robardet Dino Scaravelli Barbara Zecchin Zsófia Lanszki Tamás Görföl Gábor Kemenesi Paola De Benedictis |
| author_facet | Stefania Leopardi Laurent Dacheux Jordi Serra-Cobo Ágota Ábrahám Branka Bajić Hervé Bourhy Szilárd-Lehel Bücs Ivana Budinski Martina Castellan Petra Drzewniokova Heliana Dundarova Francesca Festa Lauriane Kergoat Maxime Leuchtmann Marc López-Roig Dominique Pontier Maria Francesca Priore Emmanuelle Robardet Dino Scaravelli Barbara Zecchin Zsófia Lanszki Tamás Görföl Gábor Kemenesi Paola De Benedictis |
| author_sort | Stefania Leopardi |
| collection | DOAJ |
| description | Summary: Among lyssaviruses, West Caucasian bat virus (WCBV) and Lleida bat lyssavirus (LLEBV) raise concern as their divergence from rabies virus leads to the inefficacy of available prophylactic agents. Both viruses were described in the bat Miniopterus schreibersii. We investigated the European distribution of WCBV and LLEBV by screening sera from Miniopterus schreibersii across eight countries, finding widespread serological evidence and positivity up to 70%. We evaluated the intramuscular lethality of wild type isolates in Syrian hamsters. WCBV induced 100% lethality and a clinical disease compatible with furious rabies. All animals infected with LLEBV remained healthy for 40 days, despite one individual testing positive in the brain. We confirmed LLEBV’s intramuscular a-pathogenicity using mice. Infected hamsters developed antibodies by day seven, regardless the virus and the clinical outcome. This study highlights the widespread circulation of WCBV and LLEBV in Europe and suggests differences in neuro-invasiveness and/or pathogenesis that are crucial for risk assessment. |
| format | Article |
| id | doaj-art-747d5915f5e64886a75ea0d68397ede2 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-747d5915f5e64886a75ea0d68397ede22025-01-18T05:05:06ZengElsevieriScience2589-00422025-02-01282111738European distribution and intramuscular pathogenicity of divergent lyssaviruses West Caucasian bat virus and Lleida bat lyssavirusStefania Leopardi0Laurent Dacheux1Jordi Serra-Cobo2Ágota Ábrahám3Branka Bajić4Hervé Bourhy5Szilárd-Lehel Bücs6Ivana Budinski7Martina Castellan8Petra Drzewniokova9Heliana Dundarova10Francesca Festa11Lauriane Kergoat12Maxime Leuchtmann13Marc López-Roig14Dominique Pontier15Maria Francesca Priore16Emmanuelle Robardet17Dino Scaravelli18Barbara Zecchin19Zsófia Lanszki20Tamás Görföl21Gábor Kemenesi22Paola De Benedictis23Laboratory for Emerging Viral Zoonoses, Istituto Zooprofilattico Sperimentale delle Venezie, Padova, Italy; Corresponding authorUnit Lyssavirus, Epidemiology and Neuropathology, Institut Pasteur, Université Paris-Cité, Paris, France; Unit Environment and Infectious Risks, Institut Pasteur, Université Paris-Cité, Paris, FranceDepartament de Biologia Evolutiva, Ecologia i Ciències Ambientals. Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain; Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona, SpainNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, HungaryDepartment of Genetic Research, Institute for Biological Research “Siniša Stanković” – National Institute of the Republic of Serbia, University of Belgrade, Belgrade, SerbiaUnit Lyssavirus, Epidemiology and Neuropathology, Institut Pasteur, Université Paris-Cité, Paris, FranceCentre for Bat Research and Conservation, Cluj Napoca, RomaniaDepartment of Genetic Research, Institute for Biological Research “Siniša Stanković” – National Institute of the Republic of Serbia, University of Belgrade, Belgrade, SerbiaLaboratory for Emerging Viral Zoonoses, Istituto Zooprofilattico Sperimentale delle Venezie, Padova, ItalyLaboratory for Emerging Viral Zoonoses, Istituto Zooprofilattico Sperimentale delle Venezie, Padova, Italy; Department of Public Health Experimental and Forensic Medicine, University of Pavia, Pavia, ItalyInstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, BulgariaLaboratory for Emerging Viral Zoonoses, Istituto Zooprofilattico Sperimentale delle Venezie, Padova, Italy; Department of Biology and Biotechnology ''Charles Darwin'', University of Rome ''La Sapienza'', Roma, ItalyUnit Lyssavirus, Epidemiology and Neuropathology, Institut Pasteur, Université Paris-Cité, Paris, FranceNature Environnement 17, Surgères, France; France Nature Environnement Nouvelle-Aquitaine, Angoulême, FranceDepartament de Biologia Evolutiva, Ecologia i Ciències Ambientals. Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain; Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona, SpainCNRS Laboratoire de Biométrie et Biologie Évolutive UMR5558, Université de Lyon Université, Lyon, France; LabEx ECOFECT Ecoevolutionary Dynamics of Infectious Diseases, Université de Lyon, Lyon, FranceLaboratory for Emerging Viral Zoonoses, Istituto Zooprofilattico Sperimentale delle Venezie, Padova, ItalyAnses, Nancy Laboratory for Rabies and Wildlife, EURL for Rabies, ANSES, Malzéville Cedex, FranceS.T.E.R.N.A., Forlì, ItalyLaboratory for Emerging Viral Zoonoses, Istituto Zooprofilattico Sperimentale delle Venezie, Padova, ItalyNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, HungaryNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, HungaryNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pécs, Hungary; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, HungaryLaboratory for Emerging Viral Zoonoses, Istituto Zooprofilattico Sperimentale delle Venezie, Padova, ItalySummary: Among lyssaviruses, West Caucasian bat virus (WCBV) and Lleida bat lyssavirus (LLEBV) raise concern as their divergence from rabies virus leads to the inefficacy of available prophylactic agents. Both viruses were described in the bat Miniopterus schreibersii. We investigated the European distribution of WCBV and LLEBV by screening sera from Miniopterus schreibersii across eight countries, finding widespread serological evidence and positivity up to 70%. We evaluated the intramuscular lethality of wild type isolates in Syrian hamsters. WCBV induced 100% lethality and a clinical disease compatible with furious rabies. All animals infected with LLEBV remained healthy for 40 days, despite one individual testing positive in the brain. We confirmed LLEBV’s intramuscular a-pathogenicity using mice. Infected hamsters developed antibodies by day seven, regardless the virus and the clinical outcome. This study highlights the widespread circulation of WCBV and LLEBV in Europe and suggests differences in neuro-invasiveness and/or pathogenesis that are crucial for risk assessment.http://www.sciencedirect.com/science/article/pii/S2589004224029651Health sciencesVirologyMicrobiology |
| spellingShingle | Stefania Leopardi Laurent Dacheux Jordi Serra-Cobo Ágota Ábrahám Branka Bajić Hervé Bourhy Szilárd-Lehel Bücs Ivana Budinski Martina Castellan Petra Drzewniokova Heliana Dundarova Francesca Festa Lauriane Kergoat Maxime Leuchtmann Marc López-Roig Dominique Pontier Maria Francesca Priore Emmanuelle Robardet Dino Scaravelli Barbara Zecchin Zsófia Lanszki Tamás Görföl Gábor Kemenesi Paola De Benedictis European distribution and intramuscular pathogenicity of divergent lyssaviruses West Caucasian bat virus and Lleida bat lyssavirus iScience Health sciences Virology Microbiology |
| title | European distribution and intramuscular pathogenicity of divergent lyssaviruses West Caucasian bat virus and Lleida bat lyssavirus |
| title_full | European distribution and intramuscular pathogenicity of divergent lyssaviruses West Caucasian bat virus and Lleida bat lyssavirus |
| title_fullStr | European distribution and intramuscular pathogenicity of divergent lyssaviruses West Caucasian bat virus and Lleida bat lyssavirus |
| title_full_unstemmed | European distribution and intramuscular pathogenicity of divergent lyssaviruses West Caucasian bat virus and Lleida bat lyssavirus |
| title_short | European distribution and intramuscular pathogenicity of divergent lyssaviruses West Caucasian bat virus and Lleida bat lyssavirus |
| title_sort | european distribution and intramuscular pathogenicity of divergent lyssaviruses west caucasian bat virus and lleida bat lyssavirus |
| topic | Health sciences Virology Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224029651 |
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