Constituyentes químicos de fracciones no polares y actividades biológicas de las hojas de Eugenia myrcianthes (Myrtaceae)

The genus Eugenia (Myrtaceae) represents an important source of medicinal species, with documented biological activities attributed to its diverse secondary metabolites, particularly phenolic compounds and terpenoids. However, comprehensive toxicological evaluation remains essential to validate the...

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Main Authors: Letizia Grau, Luis Marin, Francisco Ferreira, Elvio Gayoso, Juliana Moura - Mendes, Daniela Gamenara
Format: Article
Language:Spanish
Published: Fundación Miguel Lillo 2025-06-01
Series:Lilloa
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Online Access:https://www.lillo.org.ar/journals/index.php/lilloa/article/view/2063
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author Letizia Grau
Luis Marin
Francisco Ferreira
Elvio Gayoso
Juliana Moura - Mendes
Daniela Gamenara
author_facet Letizia Grau
Luis Marin
Francisco Ferreira
Elvio Gayoso
Juliana Moura - Mendes
Daniela Gamenara
author_sort Letizia Grau
collection DOAJ
description The genus Eugenia (Myrtaceae) represents an important source of medicinal species, with documented biological activities attributed to its diverse secondary metabolites, particularly phenolic compounds and terpenoids. However, comprehensive toxicological evaluation remains essential to validate the safety of traditional applications. This research aimed to evaluate the chemical constituents of non-polar fractions and the bioactive potential of Eugenia myrcianthes (Yva hái) leaves. Analyses were performed on hexane, chloroform, and aqueous fractions obtained from the ethanolic extract of the plant material. Phytochemical screening revealed the presence of phenolic compounds, flavonoids, tannins, saponins, and triterpenes/steroids. Gas chromatography-mass spectrometry (GC-MS) identified major bioactive constituents as Delta-cadinene, Alfa-tocopherol, phytol, and squalene in the chloroform fraction, while Tau-muurolol, stigmasterol, Beta-amyrone, and lupenyl acetate were detected in the hexane phase. Bioactivity assessment demonstrated remarkable antioxidant capacity in the aqueous portion (DPPH IC50 = 18.5±0.33 ug.mL-1), contrasting with limited antimicrobial effects against Escherichia coli, Salmonella typhimurium, Staphylococcus epidermidis, and Staphylococcus aureus strains at ?2500 ug.mL-1. Furthermore, no toxicity was detected at ?20 mg.mL-1 using Drosophila melanogaster as a model organism. These findings highlight E. myrcianthes as a promising source of natural antioxidants. Further research into its specific bioactive compounds is necessary to fully assess the plant’s therapeutic potential.
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spelling doaj-art-747cfc1efc804200a3d2b2c343970b792025-08-20T03:16:28ZspaFundación Miguel LilloLilloa2346-96412025-06-0110.30550/j.lil/2063Constituyentes químicos de fracciones no polares y actividades biológicas de las hojas de Eugenia myrcianthes (Myrtaceae)Letizia Grau0Luis Marin1Francisco Ferreira2Elvio Gayoso3Juliana Moura - Mendes4Daniela Gamenara5Universidad Nacional de Asunción, Facultad de Ciencias Exactas y Naturales, San Lorenzo - Paraguay.Universidad Nacional de Asunción, Facultad de Ciencias Exactas y Naturales, San Lorenzo - ParaguayUniversidad Nacional de Asunción, Facultad de Ciencias Exactas y Naturales, San Lorenzo - ParaguayUniversidad Nacional de Asunción, Facultad de Ciencias Exactas y Naturales, San Lorenzo - ParaguayUniversidad Nacional de Asunción, Centro Multidisciplinario de Investigaciones Tecnológicas, San Lorenzo – Paraguay.Universidad de la República, Facultad de Química, Montevideo - Uruguay. The genus Eugenia (Myrtaceae) represents an important source of medicinal species, with documented biological activities attributed to its diverse secondary metabolites, particularly phenolic compounds and terpenoids. However, comprehensive toxicological evaluation remains essential to validate the safety of traditional applications. This research aimed to evaluate the chemical constituents of non-polar fractions and the bioactive potential of Eugenia myrcianthes (Yva hái) leaves. Analyses were performed on hexane, chloroform, and aqueous fractions obtained from the ethanolic extract of the plant material. Phytochemical screening revealed the presence of phenolic compounds, flavonoids, tannins, saponins, and triterpenes/steroids. Gas chromatography-mass spectrometry (GC-MS) identified major bioactive constituents as Delta-cadinene, Alfa-tocopherol, phytol, and squalene in the chloroform fraction, while Tau-muurolol, stigmasterol, Beta-amyrone, and lupenyl acetate were detected in the hexane phase. Bioactivity assessment demonstrated remarkable antioxidant capacity in the aqueous portion (DPPH IC50 = 18.5±0.33 ug.mL-1), contrasting with limited antimicrobial effects against Escherichia coli, Salmonella typhimurium, Staphylococcus epidermidis, and Staphylococcus aureus strains at ?2500 ug.mL-1. Furthermore, no toxicity was detected at ?20 mg.mL-1 using Drosophila melanogaster as a model organism. These findings highlight E. myrcianthes as a promising source of natural antioxidants. Further research into its specific bioactive compounds is necessary to fully assess the plant’s therapeutic potential. https://www.lillo.org.ar/journals/index.php/lilloa/article/view/2063Yva háiphenolic compoundsterpenesDPPHfruit fly
spellingShingle Letizia Grau
Luis Marin
Francisco Ferreira
Elvio Gayoso
Juliana Moura - Mendes
Daniela Gamenara
Constituyentes químicos de fracciones no polares y actividades biológicas de las hojas de Eugenia myrcianthes (Myrtaceae)
Lilloa
Yva hái
phenolic compounds
terpenes
DPPH
fruit fly
title Constituyentes químicos de fracciones no polares y actividades biológicas de las hojas de Eugenia myrcianthes (Myrtaceae)
title_full Constituyentes químicos de fracciones no polares y actividades biológicas de las hojas de Eugenia myrcianthes (Myrtaceae)
title_fullStr Constituyentes químicos de fracciones no polares y actividades biológicas de las hojas de Eugenia myrcianthes (Myrtaceae)
title_full_unstemmed Constituyentes químicos de fracciones no polares y actividades biológicas de las hojas de Eugenia myrcianthes (Myrtaceae)
title_short Constituyentes químicos de fracciones no polares y actividades biológicas de las hojas de Eugenia myrcianthes (Myrtaceae)
title_sort constituyentes quimicos de fracciones no polares y actividades biologicas de las hojas de eugenia myrcianthes myrtaceae
topic Yva hái
phenolic compounds
terpenes
DPPH
fruit fly
url https://www.lillo.org.ar/journals/index.php/lilloa/article/view/2063
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